Citation: YANG Jian-Ping, ZHU Cai-Jun, LI Qiu-Na, CHEN Hua-Ying, HUANG Zhi-Xuan, LIU Zhi-Kang, LIANG Xiao, LIANG Nan-Feng. A Novel Method for Screening α-Glycosidase Inhibitors Based on Nickel-based Nanomaterial Electrochemical Sensor[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(3): 373-382. doi: 10.19756/j.issn.0253-3820.221006 shu

A Novel Method for Screening α-Glycosidase Inhibitors Based on Nickel-based Nanomaterial Electrochemical Sensor

School of Chemistry and Materials Science, Guangdong University of Education, Guangzhou 510303, China

Corresponding author: YANG Jian-Ping, yangjianping@gdei.edu.cn
Received Date: 3 January 2022
Revised Date: 16 February 2023

Fund Project: Supported by the Science and Technology Program of Guangzhou City, China (No. 202002030444), the Special Projects in Key Fields of Education Department of Guangdong Province, China (No.2022ZDZX4037), the Science and Technology Innovation Climbing Program of Guangdong Province, China (No. pdjh2021a0364) and College Students′ Innovation and Entrepreneurship Project (Nos. 202214278010, 202214278082).

To establish a novel and simple method for screening glycosidase inhibitors from traditional Chinese medicine, a nickel-based nanomaterial modified electrode (Ni(OH)₂/NF) was prepared on nickel foam (NF) in one step by multi-cycle electrochemical cyclic voltammetry, which was used to construct a highly sensitive and selective electrochemical sensor for evaluating the inhibitory activity of α-glycosidase inhibitors. The structure and surface morphology of the modified electrode were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and its sensing performance was investigated by cyclic voltammetry and chronoamperometry. The results showed that the Ni(OH)₂/NF sensor had a high electrochemical response to glucose in complex enzyme reaction system, and the sensitivity was 3222 μA·mmol/(L·cm²) in the linear range of 3.0-6000 μmol/L, with a detection limit as low as 0.9 μmol/L (S/N = 3). The feasibility of the sensor for detecting α-glycosidase activity was verified by a clinical hypoglycemic drug acarbose. The nanosensor was also applied to evaluate the inhibitory effect of lotus stamen extract. It was found that lotus stamen had a certain α-glycosidase inhibitory activity, with its half inhibition concentration IC₅₀ = 3.31 g/L. The experimental results showed that the developed sensor was suitable for analysis of α-glycosidase inhibitory activity, providing a novel method for screening natural hypoglycemic chemicals.
- Nickel-based nanosensor,
- Glycosidase inhibitors,
- Antidiabetic drug,
- Lotus stamen
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