Citation: ZHANG Qian-Wen, CHEN Qian, BIAN Xiao-Jun, YAN Juan. Aptasensor Based on Terminal Deoxynucleotidyl Transferase-Mediated Signal Amplification for Salmonella Typhimurium Detection[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(8): 1289-1299. doi: 10.19756/j.issn.0253-3820.211148 shu

Aptasensor Based on Terminal Deoxynucleotidyl Transferase-Mediated Signal Amplification for Salmonella Typhimurium Detection

ZHANG Qian-Wen ¹ ,
CHEN Qian ¹ ,
BIAN Xiao-Jun ^1,2,3 ,
YAN Juan ^1,2,3,,

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
3.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation Shanghai, Ministry of Agriculture, Shanghai 201306, China

Corresponding author: YAN Juan, j-yan@shou.edu.cn
Received Date: 1 March 2021
Revised Date: 9 May 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21775102) and Natural Science Foundation of Shanghai Municipal, China (No.20ZR1424100).

Salmonella typhimurium (S.typhimurium) is one of the most widespread pathogens causing foodborne diseases. In this work, S.typhimurium was used as the model bacteria. An aptasensor based on aldehyde magnetic beads (Mbs), enzyme digestion and terminal deoxynucleotidyl transferase-mediated signal amplification technique for simple colorimetric detection of S.typhimurium was developed. The Mbs@dsDNA hybrid complex was first prepared using the capture probe (CP) and aptamer. In the presence of S.typhimurium, the aptamer specifically bound to it, and released from the Mbs, which was then removed by magnetic separation. Subsequently, CP on Mbs folded back on itself owing to partially complementary bases and formed enzyme digestion recognition sites. 3'-OH terminal ends were obtained by EcoR I enzyme digestion. Deoxynucleotides were catalyzed to the 3'-OH end under the action of terminal deoxynucleotidyl transferase (TdT) to obtain products of single-stranded DNA (ssDNA). In the process, signal reporter molecules were embedded in the chain of ssDNA, thus obtaining amplified signals. In the absence of S.typhimurium, TdT signal amplification reaction could not be initiated because no released aptamer and blocked 3'-OH end CP. The aptasensor had a good response performance to S.typhimurium in the concentration range of 10¹-10⁵ CFU/mL, and the detection limit was as low as 21 CFU/mL, showing high sensitivity and specificity. Moreover, the recovery of S.typhimurium in milk samples was 92.2%-112.7% (RSD<3%). The aptasensor showed potential application prospect in the food safety detection and supervision of other foodborne pathogenic microorganisms and other pollutants.
- Biosensor,
- Aptamer,
- Signal amplification,
- Foodborne diseases,
- Food safety
1. [1]
  HUANG F, XUE L, ZHANG H, GUO R, LI Y, LIAO M, WANG M, LIN J. Theranostics, 2018, 8(22): 6263-6273.
2. [2]
  GE C, YUAN R, YI L, YANG J L, ZHANG H W, LI L X, NIAN W Q, YI G. J. Electroanal. Chem., 2018, 826: 174-180.
3. [3]
  BAYRAÇ C, EYIDOǦAN F, AVNI ÖKTEM H. Biosens. Bioelectron., 2017, 98: 22-28.
4. [4]
  WU S, DUAN N, QIU Y, LI J, WANG Z. Int. J. Food Microbiol., 2017, 261: 42-48.
5. [5]
  GAO M, YU F, CHEN H, CHEN L. Anal. Chem., 2015, 87(7): 3631-3638.
6. [6]
  DU F Y, GUO L, QIN Q, ZHENG X, RUAN G H, LI J P, LI G K. TrAC-Trends Anal. Chem., 2015, 67: 134-146.
7. [7]
  ZHU P Y, SHANG Y, TIAN W Y, HUANG K L, LUO Y B, XU W T. Food Chem., 2017, 221: 1770-1777.
8. [8]
  CHEN Z B, ZHANG C M, ZHOU T H, M H. Microchim. Acta, 2015, 182(5): 1003-1008.
9. [9]
  NAKATSUKA N, YANG K, ABENDROTH J, CHEUNG K, XU X B, YANG H Y, ZHAO C Z, ZHU B, RIM Y, Y Y, WEISS P, STOJANOVIG＇ M, ANDREWS A. Science, 2018, 362(6412): 319-324.
10. [10]
  MUNIANDY S, TEH S J, APPATURI J N, THONG K L, LAI C W, IBRAHIM F, LEO B F. Bioelectrochemistry, 2019, 127: 136-144.
11. [11]
  LIN Z T, GU J H, LI C H, LEE T R, XIE L X, CHEN S, CAO P Y, JIANG S, YUAN Y L, HONG X, WANG H T, WANG D Z, WANG X F, JIANG G B, HEON M, WU T F. Adv. Mater., 2017, 29(31): 1702090.
12. [12]
  BOSTAN H B, DANESH N M, KARIMI G, RAMEZANI M, SHAEGH S A M, YOUSSEFI K, CHARBGOO F, ABNOUS K, TAGHDISI S M. Biosens. Bioelectron., 2017, 98: 168-179.
13. [13]
  YANG Q, ZHOU L Y, WU Y X, ZHANG K, CAO Y T, ZHOU Y, WU D Z, HU F T, GAN N. Anal. Chim. Acta, 2018, 1020: 1-8.
14. [14]
  TIAN R, JI J Y, ZHOU Y Y, DU Y M, BIAN X J, ZHU F L, LIU G, DENG S Y, WAN Y, YAN J. Biosens. Bioelectron., 2020, 160: 112218.
15. [15]
  ZHOU Y Y, FANG W N, LAI K Q, ZHU Y H, BIAN X J. Biosens. Bioelectron., 2019, 160: 112218.
16. [16]
  LI A, ZUO P, YE B C. Anal. Biochem., 2021, 615: 114050.
17. [17]
  LIU X H, LI L B, LUO L J, BI X Y, YAN H, LI X, YOU T Y. J. Colloid Interface Sci., 2021, 586: 103-109.
18. [18]
  XIA W X, SHANGGUAN X Y, LI M, WANG Y, XI D M, SUN W, FAN J L, SHAO K, PENG X J. Chem. Sci., 2021, 12: 3314-3321.
19. [19]
  VALLEJO-PEREZ M, TERNON C, SPINELLI N, MORISOT F, THEODOROU C, JAYAKUMAR G, HELLSTROM P E, MOUIS M, RAPENNE L, MESCOT X, SALEM B, STAMBOULI V. Nanomaterials, 2020, 10(9): 1842.
20. [20]
21. [21]
  WANG L, WANG R, WANG H, SLAVIK M, WEI H, LI Y. Anal. Biochem., 2017, 533: 34-40.
22. [22]
  LIU Z L, LI W, NIE Z, PENG F F, HUANG Y, YAO S Z. Chem. Commun., 2014, 50(52): 6875-6878.
23. [23]
  DU Y M, ZHOU Y Y, WEN Y L, BIAN X J, XIE Y Y, ZHANG W J, LIU G, YAN J. Microchim. Acta, 2019, 186: 840.
24. [24]
  ZHOU Y Y, FANG W N, LAI K Q, ZHU Y H, BIAN X J, SHEN J L, LI Q, WANG L H, ZHANG W J, YAN J. Biosens. Bioelectron., 2019, 141: 11419.
25. [25]
  XU F Z, LUO L, SHI H, HE X X, LEI Y L, TANG J L, HE D G, QIAO Z Z, WANG K M. Anal. Chim. Acta, 2018: 1010: 54-61.
26. [26]
27. [27]
  DUBOWSKI J J. Sens. Actuators, B, 2014, 207: 556-562.
28. [28]
  BULARD E, BOUCHET-SPINELLI A, CHAUD P, ROGET A, CALEMCZUK R, FORT S, LIVACHE T. Anal. Chem., 2015, 87(3): 1804-1811.
29. [29]
  OZALP V C, BAYRAMOGLU G, ERDEM Z, ARICA M Y. Anal. Chim. Acta, 2015, 853: 533-540.
30. [30]
  WANG R J, XU Y, ZHANG T, JIANG Y. Anal. Methods., 2015, 7(5): 1701-1706.
31. [31]
  LI Z, YANG H, SUN L, QI H, QIANG G, ZHANG C. Sens. Actuators, B, 2015, 210: 468-474.
1. [1]
  Meiqing Yang , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-. doi: 10.3866/PKU.WHXB202310046
2. [2]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
3. [3]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
4. [4]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
5. [5]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . 倍增型全聚合物光电探测器及其在光电容积描记传感器上的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2311021-. doi: 10.3866/PKU.WHXB202311021
6. [6]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
7. [7]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
8. [8]
  Dongxue Han , Huiliang Sun , Li Niu . Virtual Reality Technology for Safe and Green University Chemistry Experimental Education. University Chemistry, 2024, 39(8): 191-196. doi: 10.3866/PKU.DXHX202312055
9. [9]
  Simin Fang , Hong Wu , Sizhe Sheng , Lingling Li , Yuxi Wang , Hongchun Li , Jun Jiang . The Food Kingdom Lecture Series: The Science behind Color. University Chemistry, 2024, 39(9): 177-182. doi: 10.12461/PKU.DXHX202402012
10. [10]
  Qilu DU , Li ZHAO , Peng NIE , Bo XU . Synthesis and characterization of osmium-germyl complexes stabilized by triphenyl ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1088-1094. doi: 10.11862/CJIC.20240006
11. [11]
  Xiao SANG , Qi LIU , Jianping LANG . Synthesis, structure, and fluorescence properties of Zn(Ⅱ) coordination polymers containing tetra-alkenylpyridine ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2124-2132. doi: 10.11862/CJIC.20240158
12. [12]
  Xiaoling LUO , Pintian ZOU , Xiaoyan WANG , Zheng LIU , Xiangfei KONG , Qun TANG , Sheng WANG . Synthesis, crystal structures, and properties of lanthanide metal-organic frameworks based on 2, 5-dibromoterephthalic acid ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1143-1150. doi: 10.11862/CJIC.20230271
13. [13]
  Yingchun ZHANG , Yiwei SHI , Ruijie YANG , Xin WANG , Zhiguo SONG , Min WANG . Dual ligands manganese complexes based on benzene sulfonic acid and 2, 2′-bipyridine: Structure and catalytic properties and mechanism in Mannich reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1501-1510. doi: 10.11862/CJIC.20240078
14. [14]
  Youlin SI , Shuquan SUN , Junsong YANG , Zijun BIE , Yan CHEN , Li LUO . Synthesis and adsorption properties of Zn(Ⅱ) metal-organic framework based on 3, 3', 5, 5'-tetraimidazolyl biphenyl ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1755-1762. doi: 10.11862/CJIC.20240061
15. [15]
  Lubing Qin , Fang Sun , Meiyin Li , Hao Fan , Likai Wang , Qing Tang , Chundong Wang , Zhenghua Tang . 原子精确的(AgPd)₂₇团簇用于硝酸盐电还原制氨：一种配体诱导策略来调控金属核. Acta Physico-Chimica Sinica, 2025, 41(1): 2403008-. doi: 10.3866/PKU.WHXB202403008
16. [16]
  Haifeng Liu , Yong Xiao , Teng Yuan , Bimin Lin , Yizhen Wang , Hui Zeng . Exploration of Safety Facility Configuration in University Chemical Depot. University Chemistry, 2024, 39(10): 182-188. doi: 10.3866/PKU.DXHX202401036
17. [17]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
18. [18]
  Wei Li , Guoqiang Feng , Ze Chang . Teaching Reform of X-ray Diffraction Using Synchrotron Radiation in Materials Chemistry. University Chemistry, 2024, 39(3): 29-35. doi: 10.3866/PKU.DXHX202308060
19. [19]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
20. [20]
  Hao Zhao , Zhen Gao , Weihong Li . Practice and Exploration of the Construction of Experimental Technician Teams of Universities in the New Period. University Chemistry, 2024, 39(4): 7-12. doi: 10.3866/PKU.DXHX202310122

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(1084)
HTML views(98)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142