Citation: LIU Xiao-Yan, ZHOU Yan, ZHENG Ting-Ting, TIAN Yang. Surface-Enhanced Raman Scattering Technology Based on TiO₂ Nanorods for Detection of Telomerase Activity in Cells[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(7): 1218-1227. doi: 10.19756/j.issn.0253-3820.211066 shu

Surface-Enhanced Raman Scattering Technology Based on TiO₂ Nanorods for Detection of Telomerase Activity in Cells

LIU Xiao-Yan ¹ ,
ZHOU Yan ² ,
ZHENG Ting-Ting ^1,, ,
TIAN Yang ^1,2,,

1.
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China;
2.
State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, China

Corresponding author: ZHENG Ting-Ting, TIAN Yang,
Received Date: 25 January 2021
Revised Date: 2 March 2021

Fund Project: Supported by the National Natural Science Foundation of China (Nos. 21827814, 21974049) and the Shanghai Rising-star Program (No. 2QA1403300)

The TiO₂ nanorods (TiO₂ NRs) were prepared by hydrothermal method, and a TiO₂ NRs non-metallic surface-enhanced Raman spectroscopy (SERS) biosensor was constructed. With copper phthalocyanine (CuPc) as the adsorbed molecule, the developed TiO₂ NRs SERS biosensor revealed remarkable Raman activity. Through experimental data and theoretical calculations, it was found that significant SERS enhancement (Enhancement factor (EF) =3.18×10⁸) of CuPc was due to the chemical mechanism (CM) based on charge transfer. By utilizing the significant Raman response of CuPc on the TiO₂ NRs and the specific recognition of telomere G-quadruplex, TiO₂ NRs was used as a SERS biosensor for quantitative and sensitive detection of telomerase activity, with a detection limit down to 2.85×10^-16 IU/L. In addition, due to the high selectivity and high sensitivity, the SERS biosensor was used to determine the telomerase activity as well as the cell numbers in Hela cells, making it an effective way to detect telomerase activity in other cells. This work not only established an approach for studying the Raman enhancement mechanism of semiconductor based on CM, but also paved a new way for the detection of related substances in clinical diagnosis and cell biomedical analysis.
- Surface enhanced Raman spectroscopy,
- TiO₂ nanorods,
- Enhanced charge transfer,
- Telomerase activity,
- Hela cells
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Surface-Enhanced Raman Scattering Technology Based on TiO₂ Nanorods for Detection of Telomerase Activity in Cells