Citation: GUO Tian-Rong, WAN Yu-Ping, SUN Li, YE Mei, XU Sen, HAN Shi-He, WU Wen-Lin. Rapid Screening and Confirmation of 108 Kinds of Veterinary Drug Residues in Livestock and Poultry Meat by Solid Phase Extraction Purification and Liquid Chromatography Coupled with Quadrupole/Orbitrap Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(2): 271-277. doi: 10.19756/j.issn.0253-3820.211059 shu

Rapid Screening and Confirmation of 108 Kinds of Veterinary Drug Residues in Livestock and Poultry Meat by Solid Phase Extraction Purification and Liquid Chromatography Coupled with Quadrupole/Orbitrap Mass Spectrometry

GUO Tian-Rong ¹ ,
WAN Yu-Ping ¹ ,
SUN Li ² ,
YE Mei ¹ ,
XU Sen ³ ,
HAN Shi-He ^2,, ,
WU Wen-Lin ^1,4,,

1.
Chengdu Institute of Food Inspection, Chengdu 611130, China;
2.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China;
3.
Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China;
4.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China

Corresponding author: HAN Shi-He, WU Wen-Lin,
Received Date: 23 January 2021
Revised Date: 12 November 2021

Fund Project: Supported by the State Administration for Market Regulation, China (No.2021YJ009), the Application Basic Research Project of Science and Technology Plan of Sichuan Province, China (No.21YYJC0962) and the Technology Innovation Research and Development Project of Chengdu, China (No.2021-YF05-00811-SN)

An analytical strategy for simultaneous and rapid screening of 108 kinds of veterinary drug residues in livestock and poultry meat was established by using solid-phase extraction (SPE) to reduce impurity interference in samples and based on ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UHPLC-Q/Orbitrap HRMS). The homogenized samples were extracted by 80% acetonitrile aqueous solution (containing 0.2% formic acid), purified by Oasis PRiME HLB solid phase extraction column, and redissolved by nitrogen blowing. The target analytes were separated from the samples by an Agilent Eclipse plus C₁₈ (3.0 mm×150 mm, 1.8 μm) chromatographic column, with acetonitrile and 0.1% acetic acid-ammonium formate (20 mmol/L) aqueous solution system as the mobile phase in gradient elution. Under the heated electrospray ionization positive and negative ions simultaneous scan mode, the target analytes were analyzed by the full scan of primary mass spectrometry plus data-dependent secondary mass spectrometry (Full MS/dd-MS²), and quantitatively detected by a matrix matching quantitative standard curve method. The results showed that 108 kinds of veterinary drugs had a good linear relationship in the range of 0.5-2000 ng/mL, and the correlation coefficients (R) were over 0.9950. The limits of detection (LODs) were 0.1-20 μg/kg, and the limits of quantitation (LOQs) were 0.2-100 μg/kg. The standard recoveries of different livestock and poultry meat under three levels of addition (n=6) were 68.7%-112.5%, and the relative standard deviation (RSDs) were 0.9%-9.7%. This method was simple, rapid and accurate, with wide coverage and high-throughput, and was suitable for rapid screening and confirmative analysis of veterinary drug residues in livestock and poultry meat.
- Livestock and poultry meat,
- Veterinary drug residues,
- Solid phase extraction purification,
- Quadrupole/orbitrap mass spectrometry,
- Rapid screening
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