Citation: ZHANG Qiang, SHI Qiu-Na, LI Lei, LI Li-Jie, SU Yue, GUO Yin-Long. Development of Thin-layer Chromatography Carbon Fiber Ionization Mass Spectrometry and Its Application in Analysis of Traditional Chinese Medicine[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(9): 1345-1354. doi: 10.19756/j.issn.0253-3820.210868 shu

Development of Thin-layer Chromatography Carbon Fiber Ionization Mass Spectrometry and Its Application in Analysis of Traditional Chinese Medicine

ZHANG Qiang ^1,2,3 ,
SHI Qiu-Na ^1,, ,
LI Lei ¹ ,
LI Li-Jie ¹ ,
SU Yue ³ ,
GUO Yin-Long ^2,,

1.
Department of Criminal Science and Technology, Henan Police College, Zhengzhou 450046, China;
2.
State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;
3.
Center for Chinese Medicine Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Corresponding author: SHI Qiu-Na, GUO Yin-Long,
Received Date: 29 November 2021
Revised Date: 8 March 2022

Fund Project: Supported by the Humanity and Social Science Youth Foundation of Ministry of Education of China (No.20YJC820062), the Henan Province Science and Technology Research Project (No.222102310354), the Key Scientific Research Projects of Higher Education Institutions in Henan Province (Nos.22B150003, 22B150004), the Humanities and Social Sciences Research Project of Henan Provincial Department of Education (No.2021-ZDJH-0113), the College-level Scientific Research Project of Henan Police College (No.HNJY-2021-45) and the Teaching Reform Research and Practice Project of Henan Police College (No.JY2021021).

To realize simple, rapid, in-situ, non-destructive analysis and identification of compounds on thin-layer chromatography, a combined technology of thin-layer chromatography with carbon fiber ionization mass spectrometry (TLC-CFI-MS) was developed, and the application of this technique in the field of Chinese medicine analysis was studied. First, to meet the needs of different types of samples or different analysis scenarios, two analysis modes of solvent-assisted TLC-CFI-MS and heat-assisted TLC-CFI-MS were designed. Then, the application of the above two analysis methods in analysis of traditional Chinese medicine was studied, and the analytical performance of the two analytical methods was examined. The limit of detection (LOD) of coumarin by solvent-assisted TLC-CFI-MS was 8 μg/mL, and the limit of quantification (LOQ) was 30 μg/mL; LOD and LOQ of heat-assisted TLC-CFI-MS were 20 ng/mL and 50 ng/mL, respectively. The heat-assisted TLC-CFI-MS demonstrated the quantitative analysis capability for coumarin in the concentration range of 0.01-1 mg/mL (R²=0.997). In addition, the application of solvent-assisted TLC-CFI-MS and heat-assisted TLC-CFI-MS in the analysis of traditional Chinese medicines was preliminarily explored. Both methods achieved rapid in-situ identification of compounds on chromatographic plates after TLC analysis of Cinnamomum cassia and Ligusticum chuanxiong. For example, coumarin and cinnamic aldehyde from Cinnamomum cassia and ligustilide, snidium lactone and ligustride A from Ligusticum chuanxiong were all identified. In addition, the coumarin content in a cinnamon sample was determined to be 1.04 mg/g by heat-assisted TLC-CFI-MS. The results suggested that TLC-CFI-MS had certain application potential in the rapid analysis of traditional Chinese medicine compounds. It would also show certain application value in the analysis of traditional Chinese medicine components, as well as quality control and evaluation.
- Thin layer chromatography,
- Carbon fiber,
- Mass spectrometry,
- Traditional Chinese medicine
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