Citation: SONG Hui-jia, ZHANG Hai-pin, ZHANG Pei, WANG Chao-feng, DOU Xiao-jie, CHEN Xiao-yi, GAO Rui-xia. Construction of Functionalized Magnetic Molecularly Imprinted Nano-platform and Its Performance in Local Castration Therapy[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(4): 602-612. doi: 10.19756/j.issn.0253-3820.210832 shu

Construction of Functionalized Magnetic Molecularly Imprinted Nano-platform and Its Performance in Local Castration Therapy

1.
School of Chemistry, Xi’an Jiaotong University, Xi’an 710049, China;2 School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China

Corresponding author: GAO Rui-xia, ruixiagao@xjtu.edu.cn
Received Date: 10 November 2021
Revised Date: 6 March 2022

Fund Project: Supported by the Natural Science Foundation of Shaanxi Province,China(Nos. 2020JM-066, 2020JQ-019)

The current clinical castration treatment drugs for prostate cancer are mainly focus on the reduction of systemic androgen or blocking their binding to androgen receptor(AR). However, due to the complexity of signal pathway activation, developing new castration methods to achieve selective removal of androgen from local tissues of prostate cancer is of great significance to enhance the therapeutic effect and reduce systemic side effects. In this work, 3-aminopropyltriethoxysilane(APTES) and octylmethoxysilane(OTMS) were used to modify magnetic nanoparticles to prepare amphiphilic nanoparticles(AO-MNPs). The prepared AO-MNPs enhanced the affinity of carriers towards template testosterone(TSTO), benefiting from the function of the amino and hydrophobic alkyl chain. And then, AO-MNPs was used as the core, TSTO as the template molecule, and dopamine(DA) as the functional monomer to prepared superparamagnetic core-shell testosterone magnetic molecularly imprinted polymers(T/AO-MMIPs) by a two-step template immobilization method. The strategy of two-step immobilization template method not only synergistically enhanced the affinity of the imprinted holes for TSTO, but also made the imprinted holes well arranged in an orderly and regular distribution on the surface of the carrier. Thus, the fabricated T/AO-MMIPs were evaluated to possess high adsorption amount and good selectivity, which played an important role in the selective removal of specific biomolecules from complex biological tissues. When T/AO-MMIPs were taken up by prostate cancer tissue through EPR, they could selectively adsorb the inter TSTO molecules in cells, thereby blocking the combination of TSTO and AR, inhibiting AR activity and exerting prostate cancer tumor growth inhibitory effect, while no obvious effect on normal cells. The as-prepared T/AO-MMIPs nanoplatform provided a new research strategy for enhancing the treatment of prostate cancer castration.
- Surface molecular imprinting,
- Functional modification,
- Specific recognition,
- Androgen receptor nuclear translocation,
- Castration therapy
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