Citation: LIN Yuan-Bin, LYU Tian-Qi, WANG Yu-Hui, LI Lin, WU Ai-Guo, CAO Zhang-Lei, ZHENG Jian-Ping. Research Progress of Single-cell RNA Sequencing Technology in Dermatological Applications[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(7): 985-994. doi: 10.19756/j.issn.0253-3820.210722 shu

Research Progress of Single-cell RNA Sequencing Technology in Dermatological Applications

1.
Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China;
2.
School of Material Science and Engineering, Shanghai University, Shanghai 200444, China

Corresponding author: CAO Zhang-Lei, ZHENG Jian-Ping,
Received Date: 13 September 2021
Revised Date: 13 May 2022

Fund Project: Supported by the Natural Science Foundation of Zhejiang Province (No.LY20B050003), Ningbo 3315 Innovation Team(No.2019A-14-C) and the Major Science and Technology Projects in Ningbo (No.2016C50009).

Single-cell RNA sequencing technology is a high-throughput sequencing technology developed in recent years. It can study cellular gene expression and identify cell heterogeneity at the single cell level, broadening people's understanding of skin development, tumor generation and immune response. Single-cell RNA sequencing studies in skin have systematically revealed the heterogeneity of skin cells, elucidated the pathogenesis of various skin diseases, and identified potential new therapeutic targets for a variety of clinical skin diseases, thus providing guidance for diagnosis and treatment. This article reviewed in detail the single-cell RNA sequencing technology and the latest research progress of this technology in the field of dermatology, aiming to improve the treatment level of skin diseases, and discussed the main challenges for the future development of this field.
- Single-cell RNA sequencing technology,
- Dermatology,
- Library preparation,
- Genetic data analysis,
- Review
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