Citation: ZHANG Yue, ZHANG Wei, WANG Xing-Hua, MA Pin-Yi, SUN Ying, SONG Da-Qian. A Ratio Fluorescent Probe Based on g-C₃N₄ Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(1): 64-72. doi: 10.19756/j.issn.0253-3820.210717 shu

A Ratio Fluorescent Probe Based on g-C₃N₄ Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A

College of Chemistry, Jilin University, Changchun 130012, China

Corresponding author: SONG Da-Qian, songdq@jlu.edu.cn
Received Date: 31 August 2021
Revised Date: 19 November 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.22074052).

A detection system based on g-C₃N₄ nanosheets, copper ions and o-phenylenediamine (OPD) was developed for detection of coenzyme A (CoA). By using chemically inert g-C₃N₄ nanosheets and oxOPD as fluorescent indicators, CoA formed a complex with Cu²⁺, which reduced the amount of free Cu²⁺ as an oxidant and quencher in the system, resulting in the OPD oxidation process being affected. Inhibition reduced the amount of oxOPD that was used as a quencher and a fluorescent indicator, resulting in the recovery of the fluorescence of g-C₃N₄ nanosheets, thereby changing the fluorescence intensity of the entire detection system. The ratio of the fluorescence emission intensity of g-C₃N₄ nanosheets to that of oxOPD (F₄₄₀/F₅₆₀) was used as the response signal for proportional fluorescence detection. Under the optimized experimental conditions, this method had a good proportional fluorescence response toward the concentration of CoA from 1.0 μmol/L to 100.0 μmol/L. The linear regression equation was F₄₄₀/F₅₆₀=0.00502C_CoA (μmol/L)+0.1079 (R²=0.984). The detection limit (LOD=3 S/m) was 0.6 μmol/L. The constructed detection system had good selectivity, and was used to detect CoA in human serum samples, which proved that the system had the potential for detection of CoA in complex biological matrices.
- g-C₃N₄ nanosheets,
- o-Phenylenediamine,
- Copper ion,
- Ratiometric fluorescence probe,
- Coenzyme A
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A Ratio Fluorescent Probe Based on g-C3N4 Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A

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通讯作者: 陈斌, bchen63@163.com

A Ratio Fluorescent Probe Based on g-C₃N₄ Nanosheets, Copper Ion and o-Phenylenediamine for Detection of Coenzyme A