Citation: SUN Pei-Pei, ZHANG Ning, ZHAO Lin-Jing, PENG Yuan-Yuan, LIU Xi-Jian, LIU Xiao-Hui, ZHAI Ya-Sen, FENG Mei-Qing. Effects of Cinnamon Oil at Subinhibitory Concentration on Lipid Homeostasis of Candida Albicans[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(1): 92-102. doi: 10.19756/j.issn.0253-3820.210716 shu

Effects of Cinnamon Oil at Subinhibitory Concentration on Lipid Homeostasis of Candida Albicans

1.
School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China;
2.
School of Pharmacy, Fudan University, Shanghai 201203, China

Corresponding author: ZHAO Lin-Jing, ljzhao@sues.edu.cn
Received Date: 31 August 2021
Revised Date: 25 October 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.31701032).

Cinnamon oil (CO) shows a good antifungal activity against Candida Spp. However, the mechanism remains unclear, especially from the metabolic perspective. In this study, the effects of CO at subinhibitory concentration on Candida albicans were investigated by traditional microbiological method and measurement of lipids profile using a high-coverage lipidomics based on ultra-performance liquid chromatography-Q exactive-mass spectrometry (UPLC-QE-MS), combined with multivariate and univariate data statistical analyses. The results showed that the cell morphology of C.albicans was obviously changed when exposure to CO at subinhibitory concentration (0.1×MIC). A total of 1281 lipids species were identified in cells of C.albicans, which belong to 45 classes in 6 categories. Among them, 55 lipid species were significantly altered under CO treatment, mainly involving 15 lipids classes such as phosphatidylethanolamine (PE) and the derivative dimethylphosphatidylethanolamine (dMePE), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylglycerol (PG), cardiolipin (CL), phosphatidylinositol (PI), ceramide (Cer) and the derivative hexosyl ceramide (Hex1Cer), triglyceride (TG), diglyceride (DG), etc. All but five lipid species were significantly down-regulated response to CO-treated treatment. The interaction network between the key lipid species with r>0.8 and p<0.05 was constructed by using hierarchical clustering analysis and correlation analysis. This study suggested that the antifungal effect of CO on C.albicans was potentially associated with the injured metabolic functions of cell membrane and mitochondria. The lipid species with significant difference expression level may be potentially metabolic markers for the inhibitory effect of CO against C.albicans.
- Lipidomics,
- Metabolic response,
- Cinnamon oil,
- Candida albicans,
- Ultra-performance liquid chromatography-Q exactive-mass spectrometry
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