Citation: LIU Ting, LIN Xiao-Hui, CHEN Qian, BIAN Xiao-Jun, YAN Juan. An Electrochemical Sensor Based on Surface Molecularly Imprinted Polymer for Detection of Salmonella Paratyphi B[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(1): 127-135. doi: 10.19756/j.issn.0253-3820.210715 shu

An Electrochemical Sensor Based on Surface Molecularly Imprinted Polymer for Detection of Salmonella Paratyphi B

LIU Ting ¹ ,
LIN Xiao-Hui ¹ ,
CHEN Qian ¹ ,
BIAN Xiao-Jun ^1,2,3,, ,
YAN Juan ^1,2,3,,

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
3.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China

Corresponding author: BIAN Xiao-Jun, YAN Juan,
Received Date: 30 August 2021
Revised Date: 24 October 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21775102) and the Natural Science Foundation of Shanghai Municipal, China (No.20ZR1424100).

Salmonella Paratyphi B (S.Paratyphi B) is one of the most widespread pathogenic bacteria causing foodborne disease. It is of great significance to construct a rapid and sensitive analysis method for detection of S.Paratyphi B. In this work, a simple, efficient and cost-effective surface bacteria-imprinted film-based impedance sensor was prepared. First of all, with S.Paratyphi B as the template and thiophene-3-ethanol as the functional monomer, the polythiophene and bacteria were co-deposited on the surface of glassy carbon electrode by cyclic voltammetry. After in situ removal of the template bacteria, a surface bacterial imprinting film was formed on the electrode surface, which could specifically recognize the target bacteria and successfully realize the label-free detection of the target bacteria. Under the optimal experimental conditions, this bacterial imprinted impedance sensor showed good analysis performance, e.g., fast recognition (10 min), wide linear range (1.0×10¹-1.0×10⁶ CFU/mL), low detection limit (approximately 2.0 CFU/mL), and good selectivity. In addition, this impedance sensor could be successfully applied to detection of S.Paratyphi B in real samples with good recovery. Compared with the existing bacterial biosensor, the impedance sensor here had many advantages such as high preparation efficiency (only 5 min for imprinted film formation), low cost and environmental friendliness, showing potential applications in detection of foodborne pathogenic bacteria.
- Salmonella Paratyphi B,
- Electrochemical polymerization,
- Surface imprinted polymer,
- Polythiophene,
- Bacterial electrochemical sensor,
- Bacteria imprinting,
- Label-free detection
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