Citation: YIN Ju-Xin, XIA Li-Ping, ZOU Zhe-Yu, MU Ying. Multiplex Digital Polymerase Chain Reaction Technology and Its Application[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(1): 25-38. doi: 10.19756/j.issn.0253-3820.210530 shu

Multiplex Digital Polymerase Chain Reaction Technology and Its Application

YIN Ju-Xin ^1,2 ,
XIA Li-Ping ² ,
ZOU Zhe-Yu ² ,
MU Ying ^2,,

1.
School of Information and Electrical Engineering, Zhejiang University City College, Hangzhou 310015, China;
2.
Research Center for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, China

Corresponding author: MU Ying, muying@zju.edu.cn
Received Date: 28 May 2021
Revised Date: 29 July 2021

Fund Project: Supported by the National Program on Key Research Project of China(No.2019YFE0103900) and the National Natural Science Foundation of China (No.32071481).

Digital polymerase chain reaction (PCR), as a nucleic acid detection technology with wide application prospect, has become one of the most accurate nucleic acid detection technology at present. Multiplex detection is an important direction for the development of digital PCR technique. With the development of microfluidic technology, multiplex digital PCR technique has become more and more mature. This paper reviewed the research progresses of multiplex digital PCR in recent years, especially summarized the implementation of multiplex digital PCR technique in the past five years, and introduced the application of multiplex digital PCR technique in hot areas such as liquid biopsy, transgenic detection, and SARS-Cov-2 detection. Finally, the issues and challenges faced by multiplex digital PCR technique were discussed and the future direction of the technology was foreseen.
- Digital polymerase chain reaction,
- Microfluidics,
- Multiplex detection,
- Review
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沈阳化工大学材料科学与工程学院沈阳 110142