Citation: WAN Lei-Lei, CHEN Qi, CHENG Si-Ming, LI Shui-Ming, WANG Yong. Study on Time Variability and Relative Stability of Urinary Peptidomics[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1546-1554. doi: 10.19756/j.issn.0253-3820.210476 shu

Study on Time Variability and Relative Stability of Urinary Peptidomics

WAN Lei-Lei ^1,3 ,
CHEN Qi ² ,
CHENG Si-Ming ^1,3 ,
LI Shui-Ming ^1,3,4 ,
WANG Yong ^1,3,4,,

1.
Shenzhen Key Laboratory of Marine Bioresources and Ecology, Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China;
2.
The Eighth Hospital Affiliated to Sun Yat-sen University, Shenzhen 518026, China;
3.
Shenzhen Bay Laboratory, Shenzhen 518055, China;
4.
Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen 518055, China

Corresponding author: WANG Yong, wyong@szu.edu.cn
Received Date: 30 April 2021
Revised Date: 12 July 2021

Fund Project: Supported by the Shenzhen Science and Technology Innovation Committee (Nos.JCYJ20170818142154551, JCYJ20200109110001818) and the Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions (No.2021SHIBS0003).

All the peptides in the urine is peptidome, which can reflect the pathological changes of the body, thereby providing potential biomarker information. At present, morning urine is most often used in the research of urinary peptidomics. However, whether its composition changes with the sampling time is not clear. In this study, the urine samples from the three healthy people at different time points were separated by the graphene oxide-lanthanum phosphate nanocomposite (LaGM) method and identified by LC-MS/MS. Protein Pilot Software 4.5 was used for database search and analysis. It was found that the peptides and proteins of urine at each time point of the same person were different, which was reflected in the change of number and sequence of peptides in high-abundance degraded proteins and "with" or "without" in low-abundance. The number of peptides detected in urine significantly increased by sampling a plurality of times in a day. On the other hand, the peptidomics was also certain stable. The degradation peptides SGSVEDQSRVLNLGPITR of uromodulin not only existed at every time point but also had higher relative strength than other sequence-related peptides. Collagen was the dominant component in morning urine (urine at 6 o'clock). Further more,the result was verified by single urines, mixed urines of different ages, and literature data. This study showed that the sampling time was the main influencing factor of urinary peptidomics. The 24-h urine samples would be more suitable for sample than the morning urine. Due to the existence of the ladder sequence and the influence of individual differences, the absolute signal intensity of peptide should not be used for direct comparison between different samples and the selection of biomarkers.
- Morning urine,
- 24-h urine,
- Peptidomics,
- Mass spectrometry,
- Uromodulin
1. [1]
  DIAS B P A, VLAHOU A, LEITE-MOREIRA A, SANTOS L L, FERREIRA R, VITORINO R. TrAC-Trends Anal. Chem., 2017, 94:200-209.
2. [2]
  SIROLLI V, PIERONI L, DILIBERATO L, URBANI A, BONOMINI M. Int. J. Mol. Sci., 2020, 21(1):96.
3. [3]
  YANG X, HU L, YE M, ZOU H. Anal. Chim. Acta, 2014, 829:40-47.
4. [4]
  TSUCHIYA T, OSAKI T, MINAMINO N, SASAKI K. J. Proteome Res., 2015, 14(11):4921-4931.
5. [5]
  BAUCA J M, MARTINEZ-MORILLO E, DIAMANDIS E P. Clin. Chem., 2014, 60(8):1052-1061.
6. [6]
  SPADA M, ANGELICO R, DIONISI-VICI C. Liver Transplant., 2019, 25(6):827-828.
7. [7]
  BLACKBURN P R, GASS J M, VAIRO F, FARNHAM K M, ATWAL H K, MACKLIN S, KLEE E W, ATWAL P S. Appl. Clin. Genet., 2017, 10:57-66.
8. [8]
  SCHUTGENS F, ROOKMAAKER M B, MARGARITIS T, RIOS A, AMMERLAAN C, JANSEN J, GIJZEN L, VORMANN M, VONK A, VIVEEN M, YENGEJ F Y, DERAKHSHAN S, DE-WINTER-DE G K, ARTEGIANI B, BOXTEL R, CUPPEN E, HENDRICKX A, HEUVEL-EIBRINK M M, HEITZER E, LANZ H, BEEKMAN J, MURK J L, MASEREEUW R, HOLSTEGE F, DROST J, VERHAAR M C, CLEVERS H. Nat. Biotechnol., 2019, 37(3):303-313.
9. [9]
  CHINELLO C, CAZZANIGA M, DE-SIO G, Smith A J, GRASSO A, ROCCO B, SIGNORINI S, GRASSO M, BOSARI S, ZOPPIS I, MAURI G, MAGNI F. J. Transl. Med., 2015, 13(1):332.
10. [10]
  PADOAN A, BASSO D, ZAMBON C, PRAYER-GALETTI T, ARRIGONI G, BOZZATO D, MOZ S, ZATTONI F, BELLOCCO R, PLEBANI M. Clin. Proteomics, 2018, 15(1):23.
11. [11]
  WANG Y, CHEN J, CHEN L, ZHENG P, XU H, LU J, ZHONG J, LEI Y, ZHOU C, MA Q, LI Y, XIE P. Psychiat. Res., 2014, 217(1-2):25-33.
12. [12]
  PREIANO M, SAVINO R, VILLELLA C, PELAIA C, TERRACCIANO R. Int. J. Mol. Sci., 2020, 21(15):5270.
13. [13]
  BRONDANI L D A, SOARES A A, RECAMONDE-MENDOZA M, DALL A A, CAMARGO J L, MONTEIRO K M, SILVEIRO S P. Sci. Rep., 2020, 10(1):1242.
14. [14]
  PEJCHINOVSKI M, SIWY J, MULLEN W, MISCHAK H, PETRI M A, BURKLY L C, WEI R. Lupus, 2018, 27(1):6-16.
15. [15]
  PETTERSSON B M, HELANDER A, BECK O. J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2016, 1035:104-110.
16. [16]
17. [17]
18. [18]
19. [19]
  TORNG S, RIGATTO C, RUSH D N, NICKERSON P, JEFFERY J R. Transplantation, 2001, 72(8):1453-1456.
20. [20]
  BAUCA J M, NEZ-MORILLO E M, DIAMANDIS E P. Clin. Chem., 2014, 8(60):1052-1061.
21. [21]
  MERCADO C I, COGSWELL M E, LORIA C M, LIU K, ALLEN N, GILLESPIE C, WANG C, BOER I H, WRIGHT J. Am. J. Clin. Nutr., 2018, 108(3):532-547.
22. [22]
  ZHANG Z Y, RAVASSA S, NKUIPOU K E, YANG W Y, KERR S M, KOECK T, CAMPBELL A, KUZNETSOVA T, MISCHAK H, PADMANABHAN S, DOMINICZAK A F, DELLES C, STAESSEN J A. J. Am. Heart Assoc., 2017, 6(8):e5432.
23. [23]
  HUANG Q, ZHANG Z, KEERV J, TRENSON S, NKUIPOU-KENFACK E, YANG W, THIJS L, VANHAECKE J, VANAELST L N L, VANCLEEMPUT J, JANSSENS S, VERHAMME P, MISCHAK H, STAESSEN J A. Nephrol. Dial. Transpl., 2019, 34(8):1336-1343.
24. [24]
25. [25]
26. [26]
  van JULIE A D, CLOTET-FREIXAS S, ZHOU J, BATRUCH I, SUN C, GLOGAUER M, RAMPOLDI L, ELIA Y, MAHMUD F H, SOCHETT E, DIAMANDIS E P, SCHOLEY J W, KONVALINKA A. Mol. Cell. Proteomics, 2020, 19(3):501-517.
27. [27]
28. [28]
  SIWY J, SCHANSTRA J P, ARGILES A, BAKKER S J L, BEIGE J, BOUCEK P, BRAND K, DELLES C, DURANTON F, FERNANDEZ-FERNANDEZ B, JANKOWSKI M, AL KHATIB M, KUNT T, LAJER M, LICHTINGHAGEN R, LINDHARDT M, MAAHS D M, MISCHAK H, MULLEN W, NAVIS G, NOUTSOU M, ORTIZ A, PERSSON F, PETRIE J R, ROOB J M, ROSSING P, RUGGENENTI P, RYCHLIK I, SERRA A L, SNELL-BERGEON J, SPASOVSKI G, STOJCEVA-TANEVA O, TRILLINI M, VON DER LEYEN H, WINKLHOFER-ROOB B M, ZVRBIG P, JANKOWSKI J. Nephrol. Dial. Transpl., 2014, 29(8):1563-1570.
29. [29]
  LING X B, SIGDEL T K, LAU K, YING L, LAU I, SCHILLING J, SARWAL M M. J. Am. Soc. Nephrol., 2010, 21(4):646-653.
30. [30]
  KROCHMAL M, KONTOSTATHI G, MAGALHÃES P, MAKRIDAKIS M, KLEIN J, HUSI H, LEIERER J, MAYER G, BASCANDS J, DENIS C, ZOIDAKIS J, ZVRBIG P, DELLES C, SCHANSTRA J P, MISCHAK H, VLAHOU A. Sci. Rep., 2017, 7:15160.
31. [31]
  LING X B, LAU K, DESHPANDE C, PARK J L, MILOJEVIC D, MACAUBAS C, XIAO C, LOPEZ-AVILA V, KANEGAYE J, BURNS J C, COHEN H, SCHILLING J, MELLINS E D. Clin. Proteomics, 2010, 6(4):175-193.
1. [1]
  Siwei Hou , Yaxin Niu , Guanglu Zhang , Yanmei Yang , Xu Wang , Zhenzhen Chen . Application of Solid-Phase Microextraction and Mass Spectrometry in Environmental Detection. University Chemistry, 2026, 41(3): 297-306. doi: 10.12461/PKU.DXHX202504078
2. [2]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
3. [3]
  Yue-Zhou Zhu , Kun Wang , Shi-Sheng Zheng , Hong-Jia Wang , Jin-Chao Dong , Jian-Feng Li . Application and Development of Electrochemical Spectroscopy Methods. Acta Physico-Chimica Sinica, 2024, 40(3): 2304040-0. doi: 10.3866/PKU.WHXB202304040
4. [4]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
5. [5]
  Zongyuan Chen , ChunSheng Shi , Yiwen Li , Ganlin Zu , Qiang Jin , Haishan Wang , Fujun Wang , Dekun Yan , Zhijun Guo , Wangsuo Wu . Measurement of Uranium Isotopes in Environmental Water Samples by Alpha-Spectroscopy: Design of an Undergraduate Radiochemistry Experiment. University Chemistry, 2025, 40(4): 353-358. doi: 10.12461/PKU.DXHX202406103
6. [6]
  Xiaoqi LAN , Wei LI , Long JING , Mengyu SU , Xiaoling LUO , Zheng LIU , Qun TANG . Synthesis, crystal structure, and spectral properties of transition-metal-organic frameworks based on thiophene carboxylic acid ligands. Chinese Journal of Inorganic Chemistry, 2026, 42(2): 309-316. doi: 10.11862/CJIC.20250212
7. [7]
  Xiaofang Li , Zhigang Wang . 调节金助催化剂的d_z²占据轨道增强光催化合成H₂O₂. Acta Physico-Chimica Sinica, 2025, 41(7): 100080-0. doi: 10.1016/j.actphy.2025.100080
8. [8]
  Zihan Lin , Wanzhen Lin , Fa-Jie Chen . Electrochemical Modifications of Native Peptides. University Chemistry, 2025, 40(3): 318-327. doi: 10.12461/PKU.DXHX202406089
9. [9]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
10. [10]
  Zhike Yang , Jinfan Xu , Junhao Chen , Zheng Yang , Fei Ding , Neil Qiang Su . AI NMR Assistant: A DP5-Based Intelligent System for NMR Spectral Interpretation. University Chemistry, 2026, 41(1): 20-28. doi: 10.12461/PKU.DXHX202506013
11. [11]
  Shumin Zhang , Yaqi Wang , Zelin Wang , Libo Wang , Changsheng An , Difa Xu . Ultrafast electron transfer at the ZIS_1−x/UCN S-scheme interface enables efficient H₂O₂ photosynthesis coupled with tetracycline degradation. Acta Physico-Chimica Sinica, 2025, 41(11): 100136-0. doi: 10.1016/j.actphy.2025.100136
12. [12]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
13. [13]
  Yixuan Zhu , Qingtong Wang , Jin Li , Lin Chen , Junlong Zhao . Blog of Oxytocin. University Chemistry, 2024, 39(9): 134-140. doi: 10.12461/PKU.DXHX202310090
14. [14]
  Yujing Chen , Hongqun Ouyang , Dan Zhao , Yanyan Chu , Zhengping Qiao . Recommendations for the Content and Instruction of the Physical Chemistry Experiment “Construction of Ternary Liquid-Liquid Phase Diagrams”. University Chemistry, 2025, 40(7): 359-366. doi: 10.12461/PKU.DXHX202409120
15. [15]
  Hanmei Lü , Xin Chen , Qifu Sun , Ning Zhao , Xiangxin Guo . Uniform Garnet Nanoparticle Dispersion in Composite Polymer Electrolytes. Acta Physico-Chimica Sinica, 2024, 40(3): 2305016-0. doi: 10.3866/PKU.WHXB202305016
16. [16]
  Xianyong Lu , Tao Hu . Developing an Innovative Inorganic Chemistry Teaching Model Based on Aerospace Specialty Characteristics. University Chemistry, 2025, 40(7): 127-131. doi: 10.12461/PKU.DXHX202409037
17. [17]
  Xinran Zhang , Siqi Liu , Yichi Chen , Qingli Zou , Qinghong Xu , Yaqin Huang . From Protein to Energy Storage Materials: Edible Gelatin Jelly Electrolyte. University Chemistry, 2025, 40(7): 255-266. doi: 10.12461/PKU.DXHX202408104
18. [18]
  Yan Zhang , Xiaoyan Cao , Yiming Li , Shuwei Xia , Mutai Bao . Comparison of Electrolyte Solutions Section in Physical Chemistry Textbooks at Home and Abroad. University Chemistry, 2025, 40(9): 303-309. doi: 10.12461/PKU.DXHX202502027
19. [19]
  Jiandong Liu , Zhijia Zhang , Kamenskii Mikhail , Volkov Filipp , Eliseeva Svetlana , Jianmin Ma . Research Progress on Cathode Electrolyte Interphase in High-Voltage Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(2): 100011-0. doi: 10.3866/PKU.WHXB202308048
20. [20]
  Dongcheng Liu , Zhong Zhang , Jinjun Tang , Youjun Fan , Xiaokun Li , Xiulin Yang , Ting Liu , Xingcan Shen . Exploration and Practice of Practical Teaching System in the Context of Developing New Quality Productive Forces. University Chemistry, 2025, 40(12): 157-162. doi: 10.12461/PKU.DXHX202509064

Get Citation

PDF

XML

Metrics

PDF Downloads(6)
Abstract views(1182)
HTML views(93)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142