Citation: WAN Lei-Lei, CHEN Qi, CHENG Si-Ming, LI Shui-Ming, WANG Yong. Study on Time Variability and Relative Stability of Urinary Peptidomics[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1546-1554. doi: 10.19756/j.issn.0253-3820.210476 shu

Study on Time Variability and Relative Stability of Urinary Peptidomics

WAN Lei-Lei ^1,3 ,
CHEN Qi ² ,
CHENG Si-Ming ^1,3 ,
LI Shui-Ming ^1,3,4 ,
WANG Yong ^1,3,4,,

1.
Shenzhen Key Laboratory of Marine Bioresources and Ecology, Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China;
2.
The Eighth Hospital Affiliated to Sun Yat-sen University, Shenzhen 518026, China;
3.
Shenzhen Bay Laboratory, Shenzhen 518055, China;
4.
Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen 518055, China

Corresponding author: WANG Yong, wyong@szu.edu.cn
Received Date: 30 April 2021
Revised Date: 12 July 2021

Fund Project: Supported by the Shenzhen Science and Technology Innovation Committee (Nos.JCYJ20170818142154551, JCYJ20200109110001818) and the Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions (No.2021SHIBS0003).

All the peptides in the urine is peptidome, which can reflect the pathological changes of the body, thereby providing potential biomarker information. At present, morning urine is most often used in the research of urinary peptidomics. However, whether its composition changes with the sampling time is not clear. In this study, the urine samples from the three healthy people at different time points were separated by the graphene oxide-lanthanum phosphate nanocomposite (LaGM) method and identified by LC-MS/MS. Protein Pilot Software 4.5 was used for database search and analysis. It was found that the peptides and proteins of urine at each time point of the same person were different, which was reflected in the change of number and sequence of peptides in high-abundance degraded proteins and "with" or "without" in low-abundance. The number of peptides detected in urine significantly increased by sampling a plurality of times in a day. On the other hand, the peptidomics was also certain stable. The degradation peptides SGSVEDQSRVLNLGPITR of uromodulin not only existed at every time point but also had higher relative strength than other sequence-related peptides. Collagen was the dominant component in morning urine (urine at 6 o'clock). Further more,the result was verified by single urines, mixed urines of different ages, and literature data. This study showed that the sampling time was the main influencing factor of urinary peptidomics. The 24-h urine samples would be more suitable for sample than the morning urine. Due to the existence of the ladder sequence and the influence of individual differences, the absolute signal intensity of peptide should not be used for direct comparison between different samples and the selection of biomarkers.
- Morning urine,
- 24-h urine,
- Peptidomics,
- Mass spectrometry,
- Uromodulin
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