Citation: XIONG Ying-Ying, CHENG Meng-Xia, LU Hao-Jie. Research Progress of N-Glycome Enrichment Methods[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(10): 1597-1606. doi: 10.19756/j.issn.0253-3820.210427 shu

Research Progress of N-Glycome Enrichment Methods

NHC Key Laboratory of Glycoconjugates Research(Fudan University), Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China

Corresponding author: LU Hao-Jie, luhaojie@fudan.edu.cn
Received Date: 9 April 2021
Revised Date: 10 August 2021

Fund Project: Supported by the Natural National Key Research and Development Program (Nos.2016YFA0501303, 2017YFA0505001) and the National Natural Science Foundation of China (No.21974025).

Glycosylation is an important post-translational modification of proteins. Modification by glycans makes the functions of proteins diverse. As one of the main glycosylation types of proteins, N-glycosylation is closely involved in many life activities and plays a critical role in the occurrence and development of diseases. Therefore, it is of great significance to study the N-glycans on glycoproteins. Mass spectrometry technology is one of the most powerful tools for studying N-glycome. However, due to the complex structure, low abundance and low ionization efficiency of glycans in mass spectrometry, the research of N-glycome based on mass spectrometry still faces great challenges. The separation and enrichment of N-glycans is essential for the analysis of N-glycans by mass spectrometry. This article briefly introduces the process of N-glycan analysis based on mass spectrometry, focusing on the overview of N-glycan separation and enrichment methods, and summarizes the advantages and disadvantages of these methods and discusses the application and contribution of these various technologies in biomedical research.
- Glycosylation,
- N-Glycan,
- Glycome,
- Separation and enrichment,
- Mass spectrometry,
- Review
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