Citation: LIU Yu-Ke, WU Long-Ji, KONG Xia, HUANG Guo-Liang, DING Jie. A Nano-ratio Fluorescence Probe for Imaging of Hypoxia in Living Cells[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(7): 1237-1244. doi: 10.19756/j.issn.0253-3820.201802 shu

A Nano-ratio Fluorescence Probe for Imaging of Hypoxia in Living Cells

China-America Cancer Research Institute, Guangdong Medical University, Dongguan 523000, China

Corresponding author: DING Jie, wenqiong_1979@163.com
Received Date: 31 December 2020
Revised Date: 18 April 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.81772982), the Educational Department of Guangdong Province, China (No.2019KTSCX049) and the Natural Science Foundation of Guangdong Province, China (No. 2021A1515012320).

Hypoxia is an important feature of solid tumors microenvironment, which is closely related to tumor highly aggressive, metastatic characteristics and poor prognosis. Therefore, it is of great significance to establish an efficient, rapid, sensitive and non-invasive method for detection of cell endogenous hypoxia and exogenous hypoxia. In this study, a ratio fluorescence probe was designed to detect endogenous and exogenous hypoxia levels, and the ratio fluorescence imaging was used to detect intracellular hypoxia. The oxygen sensitive fluorescent dye tris(4,7-biphenyl-1,10)-phenanthroline) ruthenium dichloride ([(Ru(dpp)₃)]Cl₂) was loaded on mesoporous yolk-shell organosilicon nanoparticle (MYSN) doped with fluorescein isothiocyanate (FITC). The linear response range of the probe was 5-290 μmol/L, the response time was 1 min, and the detection limit was as low as 4.68 μmol/L. Cell experiments showed that the as-prepared probe could be significantly taken up, exhibiting high specificity and high stability in fluorescence imaging of endogenous and exogenous hypoxia.
- Meupsoporous yolk-shell organosilica nanoparticle,
- Ratio fluorescence probe,
- Intracellular hypoxia
1. [1]
  SIEMANN D W, HORSMAN M R. Pharmacol. Ther., 2015, 153: 107-124.
2. [2]
  TANG W, ZHAO G. Bioorg. Med. Chem., 2020, 28(2): 115235.
3. [3]
  ZHAO C, ZENG C, YE S, DAI X, HE Q, YANG B, ZHU H. Acta Pharm. Sin. B, 2020, 10(6): 947-960.
4. [4]
  TAMADDONI A, MOHAMMADI E, SEDAGHAT F, QUJEQ D, AS’HABI A. Pharmacol. Res., 2020, 156: 104798.
5. [5]
  FENG J, BYRNE N M, JAMAL W, COULTER J A. Cancers (Basel), 2019, 11(12): 1989.
6. [6]
  CHENG M H Y, MO Y, ZHENG G. Adv. Healthc. Mater., 2021,10(2): 2001549.
7. [7]
  BUSSINK J, KAANDERS J H, VAN DER KOGEL A J. Radiother. Oncol., 2003,67(1): 3-15.
8. [8]
  AEBERSOLD D M， BEER K T, LAISSUE J, HUG S. Int. J. Radiat. Oncol. Biol. Phys., 2000, 48(1): 17-25.
9. [9]
  CHO H, ACKERSTAFF E, CARLIN S, LUPU M E, WANG Y, RIZWAN A, O’DONOGHUE J, LING C C, HUMM J L, ZANZONICO P B, KOUTCHER J A. Neoplasia, 2009, 11(3): 247-259.
10. [10]
  FAN A P, AN H, MORADI F, ROSENBERG J, ISHII Y, NARIAI T, OKAZAWA H, ZAHARCHUK G. Neuroimage, 2020, 220: 117136.
11. [11]
12. [12]
  LI Y, SUN Y, LI J, SU Q, YUAN W, DAI Y, HAN C, WANG Q, FENG W, LI F. J. Am. Chem. Soc., 2015, 137(19): 6407-6416.
13. [13]
  CUI L, ZHONG Y, ZHU W, XU Y, DU Q, WANG X, QIAN X, XIAO Y. Org. Lett., 2011, 13(5): 928-931.
14. [14]
  ZHANG K Y, GAO P, SUN G, ZHANG T, LI X, LIU S, ZHAO Q, LO K K, HUANG W. J. Am. Chem. Soc., 2018, 140(25): 7827-7834.
15. [15]
  HUANG X, SONG J, YUNG B C, HUANG X, XIONG Y, CHEN X. Chem. Soc. Rev., 2018, 47(8): 2873-2920.
16. [16]
17. [17]
  XUE F F, CHEN J F, CHEN H R. Life Sci., 2020, 63(12): 1786-1797.
18. [18]
  WU M, MENG Q, CHEN Y, ZHANG L, LI M, CAI X, LI Y, YU P, ZHANG L, SHI J. Adv. Mater., 2016, 28(10): 1963-1969.
19. [19]
  LI L, YANG Z, FAN W, HE L, CUI C, ZOU J, TANG W, JACOBSON O, WANG Z, NIU G, HU S, CHEN X. Adv. Funct. Mater., 2020, 30(4): 1907716.
20. [20]
  YANG Z, WEN J, WANG Q, LI Y, ZHAO Y, TIAN Y, WANG X, CAO X, ZHANG Y, LU G, TENG Z, ZHANG L. ACS Appl. Mater. Interfaces, 2019, 11(1): 187-194.
21. [21]
  CHEN Y, MENG Q, WU M, WANG S, XU P, CHEN H, LI Y, ZHANG L, WANG L, SHI J. J. Am. Chem. Soc., 2014, 136(46): 16326-16334.
22. [22]
  SHAMIRIAN A, AFSARI H S, HASSAN A, MILLER L W, SNEE P. T. ACS Sens., 2016, 1(10): 1244-1250.
1. [1]
  Jun LUO , Baoshu LIU , Yunchang ZHANG , Bingkai WANG , Beibei GUO , Lan SHE , Tianheng CHEN . Europium(Ⅲ) metal-organic framework as a fluorescent probe for selectively and sensitively sensing Pb²⁺ in aqueous solution. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2438-2444. doi: 10.11862/CJIC.20240240
2. [2]
  Jing SU , Bingrong LI , Yiyan BAI , Wenjuan JI , Haiying YANG , Zhefeng Fan . Highly sensitive electrochemical dopamine sensor based on a highly stable In-based metal-organic framework with amino-enriched pores. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1337-1346. doi: 10.11862/CJIC.20230414
3. [3]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
4. [4]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
5. [5]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
6. [6]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
7. [7]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
8. [8]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249
9. [9]
  Yue Wu , Jun Li , Bo Zhang , Yan Yang , Haibo Li , Xian-Xi Zhang . Research on Kinetic and Thermodynamic Transformations of Organic-Inorganic Hybrid Materials for Fluorescent Anti-Counterfeiting Application information: Introducing a Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(6): 390-399. doi: 10.3866/PKU.DXHX202403028
10. [10]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
11. [11]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
12. [12]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
13. [13]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
14. [14]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
15. [15]
  Wenxiu Yang , Jinfeng Zhang , Quanlong Xu , Yun Yang , Lijie Zhang . Bimetallic AuCu Alloy Decorated Covalent Organic Frameworks for Efficient Photocatalytic Hydrogen Production. Acta Physico-Chimica Sinica, 2024, 40(10): 2312014-. doi: 10.3866/PKU.WHXB202312014
16. [16]
  Aiai WANG , Lu ZHAO , Yunfeng BAI , Feng FENG . Research progress of bimetallic organic framework in tumor diagnosis and treatment. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1825-1839. doi: 10.11862/CJIC.20240225
17. [17]
  Feng Sha , Xinyan Wu , Ping Hu , Wenqing Zhang , Xiaoyang Luan , Yunfei Ma . Design of Course Ideology and Politics for the Comprehensive Organic Synthesis Experiment of Benzocaine. University Chemistry, 2024, 39(2): 110-115. doi: 10.3866/PKU.DXHX202307082
18. [18]
  Xinyu Zhu , Meili Pang . Application of Functional Group Addition Strategy in Organic Synthesis. University Chemistry, 2024, 39(3): 218-230. doi: 10.3866/PKU.DXHX202308106
19. [19]
  Tianyun Chen , Ruilin Xiao , Xinsheng Gu , Yunyi Shao , Qiujun Lu . Synthesis, Crystal Structure, and Mechanoluminescence Properties of Lanthanide-Based Organometallic Complexes. University Chemistry, 2024, 39(5): 363-370. doi: 10.3866/PKU.DXHX202312017
20. [20]
  Shicheng Yan . Experimental Teaching Design for the Integration of Scientific Research and Teaching: A Case Study on Organic Electrooxidation. University Chemistry, 2024, 39(11): 350-358. doi: 10.12461/PKU.DXHX202408036

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(773)
HTML views(129)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142