Citation: LIU Yu-Ke, WU Long-Ji, KONG Xia, HUANG Guo-Liang, DING Jie. A Nano-ratio Fluorescence Probe for Imaging of Hypoxia in Living Cells[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(7): 1237-1244. doi: 10.19756/j.issn.0253-3820.201802 shu

A Nano-ratio Fluorescence Probe for Imaging of Hypoxia in Living Cells

China-America Cancer Research Institute, Guangdong Medical University, Dongguan 523000, China

Corresponding author: DING Jie, wenqiong_1979@163.com
Received Date: 31 December 2020
Revised Date: 18 April 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.81772982), the Educational Department of Guangdong Province, China (No.2019KTSCX049) and the Natural Science Foundation of Guangdong Province, China (No. 2021A1515012320).

Hypoxia is an important feature of solid tumors microenvironment, which is closely related to tumor highly aggressive, metastatic characteristics and poor prognosis. Therefore, it is of great significance to establish an efficient, rapid, sensitive and non-invasive method for detection of cell endogenous hypoxia and exogenous hypoxia. In this study, a ratio fluorescence probe was designed to detect endogenous and exogenous hypoxia levels, and the ratio fluorescence imaging was used to detect intracellular hypoxia. The oxygen sensitive fluorescent dye tris(4,7-biphenyl-1,10)-phenanthroline) ruthenium dichloride ([(Ru(dpp)₃)]Cl₂) was loaded on mesoporous yolk-shell organosilicon nanoparticle (MYSN) doped with fluorescein isothiocyanate (FITC). The linear response range of the probe was 5-290 μmol/L, the response time was 1 min, and the detection limit was as low as 4.68 μmol/L. Cell experiments showed that the as-prepared probe could be significantly taken up, exhibiting high specificity and high stability in fluorescence imaging of endogenous and exogenous hypoxia.
- Meupsoporous yolk-shell organosilica nanoparticle,
- Ratio fluorescence probe,
- Intracellular hypoxia
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