Citation: CHEN Yu-Tian, REN Yi-Ping, WANG Li-Li, HUANG Zhong-Ping. Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(9): 1523-1530. doi: 10.19756/j.issn.0253-3820.201768 shu

Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products

1.
College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
2.
Yangtze Delta Region Institute of Tsinghua University, Zhejiang Analysis and Test Center, Jiaxing 314006, China

Corresponding author: WANG Li-Li, HUANG Zhong-Ping,
Received Date: 18 December 2020
Revised Date: 22 April 2021

A method based on target proteomics and ultrahigh performance liquid chromatography-high resolution mass spectrometry/tandem mass spectrometry was established for species identification of milks and dairies from cow, buffalo, yak, goat, sheep, horse, donkey and camel. Tryptic hydrolyzed peptides from 6 kinds of milk proteins in 8 species were analyzed and screened through theoretical analysis, instrument examination, investigation of interferences, linearity and digestion speed. A total of 13 specific peptides were finally chosen and applied to identification and detection of multi-species for milk mixture samples and commercial dairies. The results showed that specific peptides maintained good stability and specificity during species analysis. This method provided valuable suggestion in quality control and supervision of multi-species diary industry.
- Targeted proteomics,
- High performance liquid chromatography-mass spectrometry,
- Species identification,
- Milk protein
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