Citation: CHEN Li-Yan, WU Di. An Excited-state Intramolecular Proton Transfer-based Ratiometric Fluorescence Probe for Detection of Hypochlorite[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(8): 1350-1356. doi: 10.19756/j.issn.0253-3820.201579 shu

An Excited-state Intramolecular Proton Transfer-based Ratiometric Fluorescence Probe for Detection of Hypochlorite

CHEN Li-Yan ^1,, ,
WU Di ²

1.
College of Chemistry, Central China Normal University, Wuhan 430079, China;
2.
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China

Corresponding author: CHEN Li-Yan, clyan@mail.ccnu.edu.cn
Received Date: 22 September 2020
Revised Date: 25 March 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.22007034).

A new ratiometric fluorescence probe (HBT-CN) was developed with familiar excited state intramolecular proton transfer (ESIPT) fluorophore 2-(2'-hydroxyphenyl)-benzothiazole (HBT) as framework and diaminomeleonitrile (DMN) group as reactive site. The performance of this probe to monitor HOCl was further investigated. This probe possessed good sensitivity and with the ability to rapidly detect HOCl within seconds with a detection limit as low as 1.1×10^-7 mol/L. Furthermore, this probe displayed high specificity for sensing HOCl over other relevant analytes including reactive oxygen species (H₂O₂, tBuO, ROO·) and reactive nitrogen species (NO₂^-, NO₃^-, ONOO^-, NO·). Importantly, the cell experiments demonstrated that the probe HBT-CN had good cell membrane permeability and could monitor HOCl in live cells by dual-channel.
- Benzothiazole,
- Fluorescence probe,
- Excited state intramolecular proton transfer,
- Hypochlorite,
- Cell imaging
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