Citation: YANG Li-Juan, DING Xiao-Xue, REN Fan-Dong, CAI Fang, FU Guang-Hui, REN Da-Bing, YI Lun-Zhao, ZHANG Hong. Liquid Chromatography-Mass Spectrometry Combined with Chemometric Methods to Analyze the Metabolic Characteristics of Patients with Coronary Heart Disease and Coronary Heart Disease with Hypertension[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(10): 1649-1656. doi: 10.19756/j.issn.0253-3820.201572 shu

Liquid Chromatography-Mass Spectrometry Combined with Chemometric Methods to Analyze the Metabolic Characteristics of Patients with Coronary Heart Disease and Coronary Heart Disease with Hypertension

1.
Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650504, China;
2.
Department of Cardiology, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China;
3.
Faculty of Science, Kunming University of Science and Technology, Kunming 650504, China;
4.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650504, China

Corresponding author: YI Lun-Zhao, ZHANG Hong,
Received Date: 23 September 2020
Revised Date: 2 August 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21775058) and the Yunnan Provincial Applied Basic Research Project (Kun Medical Joint Project) (No.2017FE468(-169)).

Coronary heart disease with hypertension (CHD-HTN) is a serious threat to the life and health of patients. In this study, the plasma samples of 51 healthy controls, 21 patients with coronary heart disease (CHD) and 16 patients with CHD-HTN were used as samples, and the ultra-performance liquid chromatography-high resolution mass spectrometer was used to analyze the plasma metabolic characteristics of the two types of patients. Among them, 104 endogenous metabolites were analyzed qualitatively and quantitatively. On this basis, principal component analysis and partial least square-discriminant analysis models were established, and combined with the results of variable importance projection and one-way analysis of variance, 8, 41 and 26 characteristic metabolites were selected to distinguish between healthy controls and patients with CHD, healthy controls and patients with CHD-HTN, patients with CHD and CHD-HTN. The results of metabolic pathway analysis showed that in patients with CHD and CHD-HTN, significant changes took place in the metabolic pathways of fatty acids such as linoleic acid, as well as the biosynthesis of amino acids such as phenylalanine, tyrosine and tryptophan. Among them, amino acid metabolism showed more significant changes in patients with CHD-HTN.
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