Citation: YANG Li-Juan, DING Xiao-Xue, REN Fan-Dong, CAI Fang, FU Guang-Hui, REN Da-Bing, YI Lun-Zhao, ZHANG Hong. Liquid Chromatography-Mass Spectrometry Combined with Chemometric Methods to Analyze the Metabolic Characteristics of Patients with Coronary Heart Disease and Coronary Heart Disease with Hypertension[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(10): 1649-1656. doi: 10.19756/j.issn.0253-3820.201572 shu

Liquid Chromatography-Mass Spectrometry Combined with Chemometric Methods to Analyze the Metabolic Characteristics of Patients with Coronary Heart Disease and Coronary Heart Disease with Hypertension

1.
Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650504, China;
2.
Department of Cardiology, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China;
3.
Faculty of Science, Kunming University of Science and Technology, Kunming 650504, China;
4.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650504, China

Corresponding author: YI Lun-Zhao, ZHANG Hong,
Received Date: 23 September 2020
Revised Date: 2 August 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21775058) and the Yunnan Provincial Applied Basic Research Project (Kun Medical Joint Project) (No.2017FE468(-169)).

Coronary heart disease with hypertension (CHD-HTN) is a serious threat to the life and health of patients. In this study, the plasma samples of 51 healthy controls, 21 patients with coronary heart disease (CHD) and 16 patients with CHD-HTN were used as samples, and the ultra-performance liquid chromatography-high resolution mass spectrometer was used to analyze the plasma metabolic characteristics of the two types of patients. Among them, 104 endogenous metabolites were analyzed qualitatively and quantitatively. On this basis, principal component analysis and partial least square-discriminant analysis models were established, and combined with the results of variable importance projection and one-way analysis of variance, 8, 41 and 26 characteristic metabolites were selected to distinguish between healthy controls and patients with CHD, healthy controls and patients with CHD-HTN, patients with CHD and CHD-HTN. The results of metabolic pathway analysis showed that in patients with CHD and CHD-HTN, significant changes took place in the metabolic pathways of fatty acids such as linoleic acid, as well as the biosynthesis of amino acids such as phenylalanine, tyrosine and tryptophan. Among them, amino acid metabolism showed more significant changes in patients with CHD-HTN.
1. [1]
  LI H Y, SUN K, ZHAO R P, HU J, HAO Z R, WANG F, LU Y J, LIU F, ZHANG Y. Front. Biosci., 2017, 22:504-515.
2. [2]
3. [3]
  AU A, CHENG K K, WEI L K. Adv. Exp. Med. Biol., 2017, 956:599-613.
4. [4]
5. [5]
  FIEHN O, KOPKA J, DORMANN P, ALTMANN T, TRETHEWEY R N, WILLMITZER L. Nat. Biotechnol., 2000, 18(11):1157-1161.
6. [6]
  NICHOLSON J K, CONNELLY J, LINDON J C, HOLMES E. Nat. Rev. Drug Discovery, 2002, 1(2):153-161.
7. [7]
  CHENG S S, SHAH S H, CORWIN E J, FIEHN O, FITZGERALD R L, GERSZTEN R E, ILLIG T, RHEE E P, SRINIVAS P R, WANG T J, JAIN M. Circ.:Cardiovasc. Genet., 2017, 10(2):e000032.
8. [8]
  LI Y P, ZHANG D, HE Y, CHEN C Z, SONG C X, ZHAO Y Y, BAI Y X, WANG Y, PU J L, CHEN J Z, YANG Y J, DOU K F. Sci. Rep., 2017, 7:15357.
9. [9]
  PAYNTER N P, BALASUBRAMANIAN R, GIULIANINI F, WANG D D, TINKER L F, GOPAL S, DEIK A A, BULLOCK K, PIERCE K A, SCOTT J, MARTINEZ-GONZALEZ M A, ESTRUCH R, MANSON J E, COOKN R, ALBERT C M, CLISH C B, REXRODE K M. Circulation, 2018, 137(8):841-853.
10. [10]
  BASAK T, VARSHNEY S, HAMID Z, GHOSH S, SETH S, SENGUPTA S. J. Proteomics, 2015, 127:169-177.
11. [11]
  WANG Y F, SUN W T, ZHENG J L, XU C, WANG X, LI T Y, TANG Y D, LI Z F. J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2018, 1100:122-130.
12. [12]
  TZOULAKI I, CASTAGNE R, BOULANGE C L, KARAMAN I, CHEKMENEVA E, EVANGELOU E, EBBELS T, KALUARACHCHI M R, CHADEAU-HYAM M, MOSEN D, DEHGHAN A, MOAYYERI A, FERREIRA D L S, GUO X Q, ROTTER J I, TAYLOR K D, KAVOUSI M, dE VRIES P S, LEHNE B, LOH M, HOFMAN A, NICHOLSON J K, CHAMBERS J, GIEGER C, HOLMES E, TRACY R, KOONER J, GREENLAND P, FRANCO O H, HERRINGTON D, LINDON J C, ELLIOTT P. Eur. Heart J., 2019, 40(34):2883-2896.
13. [13]
  FAN Y, LI Y, CHEN Y, ZHAO Y J, LIU L W, LI J, WANG S L, ALOLGA R N, YIN Y, WANG X M, ZHAO D S, SHEN J H, MENG F Q, ZHOU X, XU H, HE G P, LAI M D, LI P, ZHU W, QI L W. J. Am. Coll. Cardiol., 2016, 68(12):1281-1293.
14. [14]
  KE C F, ZHU X H, ZHANG Y X, SHEN Y P. Metabolomics, 2018, 14(9):117.
15. [15]
  ZHAO H, LIU Y J, LI Z, SONG Y Q, CAI X M, LIU Y C, ZHANG T P, YANG L, LI L, GAO S, LI Y B, YU C Q. Clin. Chim. Acta, 2018, 486:192-198.
16. [16]
  MCGARRAH R W, CROWN S B, ZHANG G F, SHAH S H, NEWGARD C B. Circ. Res., 2018, 122(9):1238-1258.
17. [17]
  LOPEZ-RUIZ R, ROMERO-GONZALEZ R, FRENICH A G. TrAC-Trends Anal. Chem., 2019, 118:170-181.
18. [18]
  MISRA B B. Metabolomics, 2021, 17(5):49.
19. [19]
  XU T Y, ZHOU L, SHI Y Y, LIU L W, ZUO L H, JIA Q Q, DU S Z, KANG J, ZHANG X J, SUN Z. J. Biochem., 2018, 164(6):427-435.
20. [20]
  YUAN X M, WEN J, JIA H W, TONG L, ZHAO J, ZHAO L S, XIONG Z L. Anal. Biochem., 2020, 591:113559.
21. [21]
  GIKA H G, ZISI C, THEODORIDIS G, WILSON I D. J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2016, 1008:15-25.
22. [22]
  CHONG J, WISHART D S, XIA J G. Curr. Protoc. Bioinf., 2019, 68(1):e86.
23. [23]
  WANG L L, LIU S, YANG W G, YU H T, ZHANG L, MA P, WU P, LI X, CHO K K, XUE S, JIANG B H. Sci. Rep., 2017, 7:40146.
24. [24]
  CHEN G Y, SONG C W, JIN S N, LI S, ZHANG Y, HUANG R Z, FENG Y L, XU Y, XIANG Y, JIANG H L. Talanta, 2017, 162:530-539.
25. [25]
  GODZIEN J, CIBOROWSKI M, MARTINEZ-ALCAZAR M P, SAMCZUK P, KRETOWSKI A, BARBAS C. J. Proteome Res., 2015, 14(8):3204-3216.
26. [26]
  YI L Z, DONG N P, YUN Y H, DENG B C, REN D B, LIU S, LIANG Y Z. Anal. Chim. Acta, 2016, 914:17-34.
27. [27]
  GODZIEN J, CIBOROWSKI M, ANGULO S, BARBAS C. Electrophoresis, 2013, 34(19):2812-2826.
28. [28]
29. [29]
  YI L Z, YUAN D L, CHE Z H, LIANG Y Z, ZHOU Z G, GAO H Y, WANG Y M. Metabolomics, 2008, 4(1):30-38.
30. [30]
  CAI F, REN F D, ZHANG Y M, DING X X, FU G H, REN D B, YANG L J, CHEN N, SHANG Y, HU Y D, YI L Z, ZHANG H. J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2021, 1169:122603.
31. [31]
  NITZ K, LACY M, ATZLER D. Arterioscler., Thromb., Vasc. Biol., 2019, 39(3):319-330.
32. [32]
  JENNINGS A, MACGREGOR A, WELCH A, CHOWIENCZYK P, SPECTOR T, CASSIDY A. J. Nutr., 2015, 145(9):2130-2138.
33. [33]
  LIU G, CHEN S, ZHONG J, TENG K L, YIN Y L. Oxid. Med. Cell. Longevity, 2017, 2017:1602074.
34. [34]
  WANG F H, LIU J, DENG Q J, QI Y, WANG M, WANG Y, ZHANG X G, ZHAO D. Atherosclerosis, 2019, 286:7-13.
35. [35]
  CHEN J N, ZHANGS L, WU J X, WU S Y, XU G S, WEI D H. DNA Cell Biol., 2020, 39(1):8-15.
36. [36]
  MIRMIRAN P, BAHADORAN Z, GHASEMI A, AZIZI F. Nutr., Metab. Cardiovasc. Dis., 2017, 27(7):633-641.
37. [37]
  HUANG M N, ZHAO H, GAO S, LIU Y J, LIU Y C, ZHANG T P, CAI X M, LI Z, LI L, LI Y B, YU C Q. Clin. Chim. Acta, 2019, 497:95-103.
38. [38]
  MONTEIRO J, LESLIE M, MOGHADASIAN M H, ARENDT B M, ALLARD J P, MA D W L. Food Funct., 2014, 5(3):426-435.
39. [39]
  SONNWEBER T, PIZZINI A, NAIRZ M, WEISS G, TANCEVSKI I. Int. J. Mol. Sci., 2018, 19(11):3285.
40. [40]
1. [1]
  Mi Wen , Baoshuo Jia , Yongqi Chai , Tong Wang , Jianbo Liu , Hailong Wu . Improvement of Fluorescence Quantitative Analysis Experiment: Simultaneous Determination of Rhodamine 6G and Rhodamine 123 in Food Using Chemometrics-Assisted Three-Dimensional Fluorescence Method. University Chemistry, 2025, 40(4): 390-398. doi: 10.12461/PKU.DXHX202405147
2. [2]
  Qian Wu , Yuanxia Lv , Zixuan Guo , Zhihao Zhao , Zhimin Zhang , Hongmei Lu . A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses. University Chemistry, 2025, 40(10): 194-202. doi: 10.12461/PKU.DXHX202411063
3. [3]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
4. [4]
  Yue-Zhou Zhu , Kun Wang , Shi-Sheng Zheng , Hong-Jia Wang , Jin-Chao Dong , Jian-Feng Li . Application and Development of Electrochemical Spectroscopy Methods. Acta Physico-Chimica Sinica, 2024, 40(3): 2304040-0. doi: 10.3866/PKU.WHXB202304040
5. [5]
  Yujing Chen , Hongqun Ouyang , Dan Zhao , Yanyan Chu , Zhengping Qiao . Recommendations for the Content and Instruction of the Physical Chemistry Experiment “Construction of Ternary Liquid-Liquid Phase Diagrams”. University Chemistry, 2025, 40(7): 359-366. doi: 10.12461/PKU.DXHX202409120
6. [6]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085
7. [7]
  Weitai Wu , Laiying Zhang , Yuan Chun , Liang Qiao , Bin Ren . Course Design of Chemical Measurement Experiments in Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 64-68. doi: 10.12461/PKU.DXHX202409031
8. [8]
  Tianyu Feng , Guifang Jia , Peng Zou , Jun Huang , Zhanxia Lü , Zhen Gao , Chu Wang . Construction of the Chemistry Biology Experiment Course in the Chemistry “101 Program”. University Chemistry, 2024, 39(10): 69-77. doi: 10.12461/PKU.DXHX202409002
9. [9]
  Laiying Zhang , Weitai Wu , Yiru Wang , Shunliu Deng , Zhaobin Chen , Jiajia Chen , Bin Ren . Practices for Improving the Course of Chemical Measurement Experiments in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 107-112. doi: 10.12461/PKU.DXHX202409032
10. [10]
  Yi-Lin Xie , Jian-Ji Zhong , Qing-Xiao Tong , Jing-Xin Jian . Exploring “Magic Teaching” as a Means to Integrate Organic Chemistry Experiments with the “Industry-University-Research” Model. University Chemistry, 2025, 40(5): 252-260. doi: 10.12461/PKU.DXHX202407024
11. [11]
  Linghua Chen . 基于双联动“三学”模式的食品专业分析化学教学改革. University Chemistry, 2025, 40(8): 78-91. doi: 10.12461/PKU.DXHX202409095
12. [12]
  Shangwen Luo , Jianguo Fang , Yanlong Yang , Shihui Dong . 化学生物学课程双语教学实践与探索. University Chemistry, 2025, 40(8): 124-129. doi: 10.12461/PKU.DXHX202410096
13. [13]
  Daxin Liang , Yudong Li , Haiyue Yang , Bailing Chen , Zhiming Liu , Chengyu Wang . Reform and Practice of an AI-Empowered “Learning-Understanding-Application” Training Model in Materials Chemistry Education. University Chemistry, 2025, 40(9): 253-263. doi: 10.12461/PKU.DXHX202503020
14. [14]
  Weigang Zhu , Jianfeng Wang , Qiang Qi , Jing Li , Zhicheng Zhang , Xi Yu . Curriculum Development for Cheminformatics and AI-Driven Chemistry Theory toward an Intelligent Era. University Chemistry, 2025, 40(9): 34-42. doi: 10.12461/PKU.DXHX202412002
15. [15]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
16. [16]
  Zhenming Xu , Yibo Wang , Zhenhui Liu , Duo Chen , Mingbo Zheng , Laifa Shen . Experimental Design of Computational Materials Science and Computational Chemistry Courses Based on the Bohrium Scientific Computing Cloud Platform. University Chemistry, 2025, 40(3): 36-41. doi: 10.12461/PKU.DXHX202403096
17. [17]
  Fangfang Chen , Haiming Fan , Yan Li , Yuan He . 化学生物学专业多元化人才培养导向的课程体系优化探索. University Chemistry, 2025, 40(8): 92-99. doi: 10.12461/PKU.DXHX202409108
18. [18]
  Weiwei Zhang , Yongxin Ren , Hong Zhang , Ke Lu . Current Situation and Quality Improvement Measures of Undergraduate Education in Modern Analytical Testing Technology under the “Learning, Teaching, and Practicing” Trinity Concept. University Chemistry, 2025, 40(10): 78-85. doi: 10.12461/PKU.DXHX202412006
19. [19]
  Zhen Shen , Yi Wang , Chen Lin , Kin Shing Chan . 南京大学化学生物学专业本科生有机化学英文教学经验. University Chemistry, 2025, 40(6): 43-47. doi: 10.12461/PKU.DXHX202407083
20. [20]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113

Get Citation

PDF

XML

Metrics

PDF Downloads(14)
Abstract views(883)
HTML views(54)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142