Citation: YANG Li-Juan, DING Xiao-Xue, REN Fan-Dong, CAI Fang, FU Guang-Hui, REN Da-Bing, YI Lun-Zhao, ZHANG Hong. Liquid Chromatography-Mass Spectrometry Combined with Chemometric Methods to Analyze the Metabolic Characteristics of Patients with Coronary Heart Disease and Coronary Heart Disease with Hypertension[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(10): 1649-1656. doi: 10.19756/j.issn.0253-3820.201572 shu

Liquid Chromatography-Mass Spectrometry Combined with Chemometric Methods to Analyze the Metabolic Characteristics of Patients with Coronary Heart Disease and Coronary Heart Disease with Hypertension

1.
Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650504, China;
2.
Department of Cardiology, The First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China;
3.
Faculty of Science, Kunming University of Science and Technology, Kunming 650504, China;
4.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650504, China

Corresponding author: YI Lun-Zhao, ZHANG Hong,
Received Date: 23 September 2020
Revised Date: 2 August 2021

Fund Project: Supported by the National Natural Science Foundation of China (No.21775058) and the Yunnan Provincial Applied Basic Research Project (Kun Medical Joint Project) (No.2017FE468(-169)).

Coronary heart disease with hypertension (CHD-HTN) is a serious threat to the life and health of patients. In this study, the plasma samples of 51 healthy controls, 21 patients with coronary heart disease (CHD) and 16 patients with CHD-HTN were used as samples, and the ultra-performance liquid chromatography-high resolution mass spectrometer was used to analyze the plasma metabolic characteristics of the two types of patients. Among them, 104 endogenous metabolites were analyzed qualitatively and quantitatively. On this basis, principal component analysis and partial least square-discriminant analysis models were established, and combined with the results of variable importance projection and one-way analysis of variance, 8, 41 and 26 characteristic metabolites were selected to distinguish between healthy controls and patients with CHD, healthy controls and patients with CHD-HTN, patients with CHD and CHD-HTN. The results of metabolic pathway analysis showed that in patients with CHD and CHD-HTN, significant changes took place in the metabolic pathways of fatty acids such as linoleic acid, as well as the biosynthesis of amino acids such as phenylalanine, tyrosine and tryptophan. Among them, amino acid metabolism showed more significant changes in patients with CHD-HTN.
1. [1]
  LI H Y, SUN K, ZHAO R P, HU J, HAO Z R, WANG F, LU Y J, LIU F, ZHANG Y. Front. Biosci., 2017, 22:504-515.
2. [2]
3. [3]
  AU A, CHENG K K, WEI L K. Adv. Exp. Med. Biol., 2017, 956:599-613.
4. [4]
5. [5]
  FIEHN O, KOPKA J, DORMANN P, ALTMANN T, TRETHEWEY R N, WILLMITZER L. Nat. Biotechnol., 2000, 18(11):1157-1161.
6. [6]
  NICHOLSON J K, CONNELLY J, LINDON J C, HOLMES E. Nat. Rev. Drug Discovery, 2002, 1(2):153-161.
7. [7]
  CHENG S S, SHAH S H, CORWIN E J, FIEHN O, FITZGERALD R L, GERSZTEN R E, ILLIG T, RHEE E P, SRINIVAS P R, WANG T J, JAIN M. Circ.:Cardiovasc. Genet., 2017, 10(2):e000032.
8. [8]
  LI Y P, ZHANG D, HE Y, CHEN C Z, SONG C X, ZHAO Y Y, BAI Y X, WANG Y, PU J L, CHEN J Z, YANG Y J, DOU K F. Sci. Rep., 2017, 7:15357.
9. [9]
  PAYNTER N P, BALASUBRAMANIAN R, GIULIANINI F, WANG D D, TINKER L F, GOPAL S, DEIK A A, BULLOCK K, PIERCE K A, SCOTT J, MARTINEZ-GONZALEZ M A, ESTRUCH R, MANSON J E, COOKN R, ALBERT C M, CLISH C B, REXRODE K M. Circulation, 2018, 137(8):841-853.
10. [10]
  BASAK T, VARSHNEY S, HAMID Z, GHOSH S, SETH S, SENGUPTA S. J. Proteomics, 2015, 127:169-177.
11. [11]
  WANG Y F, SUN W T, ZHENG J L, XU C, WANG X, LI T Y, TANG Y D, LI Z F. J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2018, 1100:122-130.
12. [12]
  TZOULAKI I, CASTAGNE R, BOULANGE C L, KARAMAN I, CHEKMENEVA E, EVANGELOU E, EBBELS T, KALUARACHCHI M R, CHADEAU-HYAM M, MOSEN D, DEHGHAN A, MOAYYERI A, FERREIRA D L S, GUO X Q, ROTTER J I, TAYLOR K D, KAVOUSI M, dE VRIES P S, LEHNE B, LOH M, HOFMAN A, NICHOLSON J K, CHAMBERS J, GIEGER C, HOLMES E, TRACY R, KOONER J, GREENLAND P, FRANCO O H, HERRINGTON D, LINDON J C, ELLIOTT P. Eur. Heart J., 2019, 40(34):2883-2896.
13. [13]
  FAN Y, LI Y, CHEN Y, ZHAO Y J, LIU L W, LI J, WANG S L, ALOLGA R N, YIN Y, WANG X M, ZHAO D S, SHEN J H, MENG F Q, ZHOU X, XU H, HE G P, LAI M D, LI P, ZHU W, QI L W. J. Am. Coll. Cardiol., 2016, 68(12):1281-1293.
14. [14]
  KE C F, ZHU X H, ZHANG Y X, SHEN Y P. Metabolomics, 2018, 14(9):117.
15. [15]
  ZHAO H, LIU Y J, LI Z, SONG Y Q, CAI X M, LIU Y C, ZHANG T P, YANG L, LI L, GAO S, LI Y B, YU C Q. Clin. Chim. Acta, 2018, 486:192-198.
16. [16]
  MCGARRAH R W, CROWN S B, ZHANG G F, SHAH S H, NEWGARD C B. Circ. Res., 2018, 122(9):1238-1258.
17. [17]
  LOPEZ-RUIZ R, ROMERO-GONZALEZ R, FRENICH A G. TrAC-Trends Anal. Chem., 2019, 118:170-181.
18. [18]
  MISRA B B. Metabolomics, 2021, 17(5):49.
19. [19]
  XU T Y, ZHOU L, SHI Y Y, LIU L W, ZUO L H, JIA Q Q, DU S Z, KANG J, ZHANG X J, SUN Z. J. Biochem., 2018, 164(6):427-435.
20. [20]
  YUAN X M, WEN J, JIA H W, TONG L, ZHAO J, ZHAO L S, XIONG Z L. Anal. Biochem., 2020, 591:113559.
21. [21]
  GIKA H G, ZISI C, THEODORIDIS G, WILSON I D. J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2016, 1008:15-25.
22. [22]
  CHONG J, WISHART D S, XIA J G. Curr. Protoc. Bioinf., 2019, 68(1):e86.
23. [23]
  WANG L L, LIU S, YANG W G, YU H T, ZHANG L, MA P, WU P, LI X, CHO K K, XUE S, JIANG B H. Sci. Rep., 2017, 7:40146.
24. [24]
  CHEN G Y, SONG C W, JIN S N, LI S, ZHANG Y, HUANG R Z, FENG Y L, XU Y, XIANG Y, JIANG H L. Talanta, 2017, 162:530-539.
25. [25]
  GODZIEN J, CIBOROWSKI M, MARTINEZ-ALCAZAR M P, SAMCZUK P, KRETOWSKI A, BARBAS C. J. Proteome Res., 2015, 14(8):3204-3216.
26. [26]
  YI L Z, DONG N P, YUN Y H, DENG B C, REN D B, LIU S, LIANG Y Z. Anal. Chim. Acta, 2016, 914:17-34.
27. [27]
  GODZIEN J, CIBOROWSKI M, ANGULO S, BARBAS C. Electrophoresis, 2013, 34(19):2812-2826.
28. [28]
29. [29]
  YI L Z, YUAN D L, CHE Z H, LIANG Y Z, ZHOU Z G, GAO H Y, WANG Y M. Metabolomics, 2008, 4(1):30-38.
30. [30]
  CAI F, REN F D, ZHANG Y M, DING X X, FU G H, REN D B, YANG L J, CHEN N, SHANG Y, HU Y D, YI L Z, ZHANG H. J. Chromatogr. B:Anal. Technol. Biomed. Life Sci., 2021, 1169:122603.
31. [31]
  NITZ K, LACY M, ATZLER D. Arterioscler., Thromb., Vasc. Biol., 2019, 39(3):319-330.
32. [32]
  JENNINGS A, MACGREGOR A, WELCH A, CHOWIENCZYK P, SPECTOR T, CASSIDY A. J. Nutr., 2015, 145(9):2130-2138.
33. [33]
  LIU G, CHEN S, ZHONG J, TENG K L, YIN Y L. Oxid. Med. Cell. Longevity, 2017, 2017:1602074.
34. [34]
  WANG F H, LIU J, DENG Q J, QI Y, WANG M, WANG Y, ZHANG X G, ZHAO D. Atherosclerosis, 2019, 286:7-13.
35. [35]
  CHEN J N, ZHANGS L, WU J X, WU S Y, XU G S, WEI D H. DNA Cell Biol., 2020, 39(1):8-15.
36. [36]
  MIRMIRAN P, BAHADORAN Z, GHASEMI A, AZIZI F. Nutr., Metab. Cardiovasc. Dis., 2017, 27(7):633-641.
37. [37]
  HUANG M N, ZHAO H, GAO S, LIU Y J, LIU Y C, ZHANG T P, CAI X M, LI Z, LI L, LI Y B, YU C Q. Clin. Chim. Acta, 2019, 497:95-103.
38. [38]
  MONTEIRO J, LESLIE M, MOGHADASIAN M H, ARENDT B M, ALLARD J P, MA D W L. Food Funct., 2014, 5(3):426-435.
39. [39]
  SONNWEBER T, PIZZINI A, NAIRZ M, WEISS G, TANCEVSKI I. Int. J. Mol. Sci., 2018, 19(11):3285.
40. [40]
1. [1]
  Mi Wen , Baoshuo Jia , Yongqi Chai , Tong Wang , Jianbo Liu , Hailong Wu . Improvement of Fluorescence Quantitative Analysis Experiment: Simultaneous Determination of Rhodamine 6G and Rhodamine 123 in Food Using Chemometrics-Assisted Three-Dimensional Fluorescence Method. University Chemistry, 2025, 40(4): 390-398. doi: 10.12461/PKU.DXHX202405147
2. [2]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
3. [3]
  Yujing Chen , Hongqun Ouyang , Dan Zhao , Yanyan Chu , Zhengping Qiao . Recommendations for the Content and Instruction of the Physical Chemistry Experiment “Construction of Ternary Liquid-Liquid Phase Diagrams”. University Chemistry, 2025, 40(7): 359-366. doi: 10.12461/PKU.DXHX202409120
4. [4]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085
5. [5]
  Weitai Wu , Laiying Zhang , Yuan Chun , Liang Qiao , Bin Ren . Course Design of Chemical Measurement Experiments in Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 64-68. doi: 10.12461/PKU.DXHX202409031
6. [6]
  Tianyu Feng , Guifang Jia , Peng Zou , Jun Huang , Zhanxia Lü , Zhen Gao , Chu Wang . Construction of the Chemistry Biology Experiment Course in the Chemistry “101 Program”. University Chemistry, 2024, 39(10): 69-77. doi: 10.12461/PKU.DXHX202409002
7. [7]
  Laiying Zhang , Weitai Wu , Yiru Wang , Shunliu Deng , Zhaobin Chen , Jiajia Chen , Bin Ren . Practices for Improving the Course of Chemical Measurement Experiments in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 107-112. doi: 10.12461/PKU.DXHX202409032
8. [8]
  Yi-Lin Xie , Jian-Ji Zhong , Qing-Xiao Tong , Jing-Xin Jian . Exploring “Magic Teaching” as a Means to Integrate Organic Chemistry Experiments with the “Industry-University-Research” Model. University Chemistry, 2025, 40(5): 252-260. doi: 10.12461/PKU.DXHX202407024
9. [9]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
10. [10]
  Zhenming Xu , Yibo Wang , Zhenhui Liu , Duo Chen , Mingbo Zheng , Laifa Shen . Experimental Design of Computational Materials Science and Computational Chemistry Courses Based on the Bohrium Scientific Computing Cloud Platform. University Chemistry, 2025, 40(3): 36-41. doi: 10.12461/PKU.DXHX202403096
11. [11]
  Zhen Shen , Yi Wang , Chen Lin , Kin Shing Chan . 南京大学化学生物学专业本科生有机化学英文教学经验. University Chemistry, 2025, 40(6): 43-47. doi: 10.12461/PKU.DXHX202407083
12. [12]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
13. [13]
  Xianfei Chen , Wentao Zhang , Haiying Du . Experimental Design of Computational Materials Science Based on Scientific Research Cases. University Chemistry, 2025, 40(3): 52-61. doi: 10.3866/PKU.DXHX202403112
14. [14]
  Yan Su , Xiuyun Wang , Huimin Guo , Yanjuan Zhang , Xinwen Zhang , Yunting Shang , Wenfeng Jiang . To Cultivate Scientific Literacy by Learning, Thinking, Practicing and Understanding, To Utilize the “Smart Eye” Expertise by Integrating of Knowledge and Action: Ideological and Political Construction of Analytical Chemistry Experiment Course. University Chemistry, 2024, 39(2): 196-202. doi: 10.3866/PKU.DXHX202308003
15. [15]
  Qiying Xia , Guokui Liu , Yunzhi Li , Yaoyao Wei , Xia Leng , Guangli Zhou , Aixiang Wang , Congcong Mi , Dengxue Ma . Construction and Practice of “Teaching-Learning-Assessment Integration” Model Based on Outcome Orientation: Taking “Structural Chemistry” as an Example. University Chemistry, 2024, 39(10): 361-368. doi: 10.3866/PKU.DXHX202311007
16. [16]
  Xinyan Chen , Meng Xiao , Fei Cai , Junxian Guo , Tianfeng Chen , Li Ma . Transformation of Scientific Research Achievements Facilitating the Construction of Experimental Courses in Frontier Interdisciplinary Disciplines: A Case of “Comprehensive Experiments in Chemical Biology”. University Chemistry, 2025, 40(7): 373-379. doi: 10.12461/PKU.DXHX202408105
17. [17]
  Peihong Fan , Hongxiang Lou . 研究生高等天然药物化学课程的教学改革探索——导学互促式混合课堂教学与自主学习能力培养. University Chemistry, 2025, 40(6): 16-21. doi: 10.12461/PKU.DXHX202407078
18. [18]
  Hongwei Ma , Fang Zhang , Hui Ai , Niu Zhang , Shaochun Peng , Hui Li . Integrated Crystallographic Teaching with X-ray,TEM and STM. University Chemistry, 2024, 39(3): 5-17. doi: 10.3866/PKU.DXHX202308107
19. [19]
  Jiantao Zai , Hongjin Chen , Xiao Wei , Li Zhang , Li Ma , Xuefeng Qian . The Learning-Centered Problem-Oriented Experimental Teaching. University Chemistry, 2024, 39(4): 40-47. doi: 10.3866/PKU.DXHX202309023
20. [20]
  Jia Zhou , Huaying Zhong . Experimental Design of Computational Materials Science Combined with Machine Learning. University Chemistry, 2025, 40(3): 171-177. doi: 10.12461/PKU.DXHX202406004

Get Citation

PDF

XML

Metrics

PDF Downloads(14)
Abstract views(764)
HTML views(50)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142