Citation: LU Jia-Qi, YE Tai, XU Fei, CAO Hui, YUAN Min, YU Jin-Song, YIN Feng-Qin, WU Xiu-Xiu. DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides[J]. Chinese Journal of Analytical Chemistry, ;2021, 49(2): 226-236. doi: 10.19756/j.issn.0253-3820.201438 shu

DNAzyme Dependent Self-Powered DNA Machine for Determination of Organophosphorus Pesticides

Shanghai Engineering Research Center for Food Rapid Detection, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: YE Tai, taiye@usst.edu.cn
Received Date: 27 July 2020
Revised Date: 30 December 2020

Fund Project: Supported by the National Natural Science Foundation of China (No. 31801636) and the Shanghai Sailing Program (No. 18YF1417300).

A self-powered DNA machine was constructed by loading DNAzyme strand and dye-labelled substrate strand co-modified gold nanoparticles (AuNPs) on MnO₂ nanosheets via van der Waals force. When acetylcholinesterase (AChE) converted its substrate, acetylthiocholine (ATCh), into thiocholine (TCh), the MnO₂ nanosheets were degraded into Mn²⁺. The Mn²⁺ dependent-DNAzyme was triggered and plenty of dye-labelled fragments were released from the surface of AuNPs, which would lead to the enhancement of fluorescence. When AChE was inhibited by organophosphorus pesticides (OPs), the DNA machine could not be activated. This self-powered DNA machine was characterized by transmission electron microscopy (TEM), ultraviolet-visible absorption spectroscopy, zeta potential, dynamic light scattering (DLS) and X-ray photoelectron spectroscopy (XPS), and the performance of DNA machine was optimized. Under the optimal conditions, the proposed DNA machine was applied to the determination of dichlorvos (DDVP) with a linear range of 5-500 μg/L and a limit of detection of 3.62 μg/L (3σ). The method presented here was applied to the recovery assay of DDVP in spiked lettuce. The recoveries for DDVP were 96.5%-104.2%, and the relative standard deviations (RSD) were 1.7%-7.5%.
- Organophosphorus pesticide,
- DNA machine,
- MnO₂ nanosheets,
- DNAzyme
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