Citation: TIAN Run, TAO Qing, BIAN Xiao-Jun, ZHU Fu-Lin, YAN Juan. Aptasensor Based on Hybrid Chain Reaction for Colorimetric Detection of Kanamycin[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(5): 608-614. doi: 10.19756/j.issn.0253-3820.191708 shu

Aptasensor Based on Hybrid Chain Reaction for Colorimetric Detection of Kanamycin

TIAN Run ^1, ,
TAO Qing ¹ ,
BIAN Xiao-Jun ^1,2,3 ,
ZHU Fu-Lin ¹ ,
YAN Juan ^1,2,3

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China;
3.
Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China

Received Date: 2 December 2019
Revised Date: 10 February 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21775102).

The colorimetric aptasensors based on magnetic particle and hybridization chain reaction (HCR) for detection of kanamycin was developed. Kanamycin, an aminoglycoside antibiotic, is extensively used to treat bacterial infections by interrupting protein synthesis. However, the abuse and overuse of kanamycin may cause serious side effects, such as kidney damage, dizziness and hearing loss and so on. In this study, a kind of Mbs@ds-DNA complexes was prepared. The kanamycin aptamer on the Mbs@ds-DNA complexes could bind to kanamycin with strong specificity, while the complementary strand of kanamycin aptamer was used as a signal probe to initiate hybrid chain reaction (HCR). The product of HCR (dsDNA) provided less stabilization without ssDNA sticky ends, and the gold nanoparticles (AuNPs) would aggregate and exhibit a color change from red to purple under the condition of high salt, as well the UV/vis absorption spectra was also changed. The data showed that this method has high sensitivity and specificity for the detection of kanamycin. The linear range was 1.6-32.0 nmol/L with a detection limit of 0.9 nmol/L (S/N=3). Additionally, the proposed aptasensor was successfully utilized for detection of kanamycin in milk and honey samples, with recovery of 96.0%-105.0%, showing good practicability. Aptamer could specifically recognize and bind to target molecules, and therefore this assay provided the reference value for the detection of other targets, showing good potential in the aspects of food safety control and prevention.
- Kanamycin,
- Hybridization chain reaction,
- Aptamer,
- Gold nanoparticles,
- Food safety
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