Citation: DENG Yan, MA Yu-Chan, WANG Min, GU Men-Qiao, CHI Kuan-Neng, HU Rong, YANG Yun-Hui. Ratio Type C-reactive Protein Immunosensor Based on Gold Nanoparticles/Fe-MIL-88-NH₂[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(4): 498-506. doi: 10.19756/j.issn.0253-3820.191662 shu

Ratio Type C-reactive Protein Immunosensor Based on Gold Nanoparticles/Fe-MIL-88-NH₂

College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China

Received Date: 11 November 2019
Revised Date: 6 February 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21465026, 21865026)

A dual-signal proportional immunosensor for detection of C-reactive protein was developed using gold-nanoparticles (AuNPs) functionalized metal-organic frameworks (MOFs) material (AuNPs /Fe-MIL-88-NH₂) as an immobilized matrix and signal unit. The octahedral Fe-MOFs can not only provide a larger effective surface area, but also increase the amount of immobilized biomolecules and promote the transport of electrons and ions, and also exhibit good electrical conductivity. Modification of AuNPs onto Fe-MOFs can further increase specific surface area to capture large amounts of antibodies and increase electron transfer capacity. With increase of CRP concentration, the oxidation peak current of K₃Fe(CN)₆/K₄Fe(CN)₆ decreases, while the oxidation peak current in Fe-MOFs is relatively constant. The response current ratio of K₃Fe(CN)₆/K₄ Fe(CN)₆ to Fe-MOFs has been used as a quantitative measurement of CRP response signal. Fe-MOFs modified by K₃Fe(CN)₆/K₄Fe(CN)₆ as a signal probe (K₃Fe(CN)₆/K₄Fe(CN)₆-sP) and AuNPs as internal reference probes (Fe-MOFs-rP). This ratio probe integrates sP and rP into one structure, ensuring identical modification conditions and the interdependence of sP and rP on a sensing interface. Therefore, this ratiometric probe has a stronger ability to eliminate environmental variation interference. The linear response of the immunosensor proposed here ranges from 1 to 200 ng/mL, and the detection limit is 0.3 ng/mL (S/N=3). The sensor has good selectivity and is expected to be used for early screening and diagnosis of cardiovascular disease. The method also lays the foundation for the preparation of other ratiometric signal amplification sensors for detecting biomarkers.
- C-reactive protein,
- Metal organic framework,
- Ratio immunosensor
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沈阳化工大学材料科学与工程学院沈阳 110142

Ratio Type C-reactive Protein Immunosensor Based on Gold Nanoparticles/Fe-MIL-88-NH2

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通讯作者: 陈斌, bchen63@163.com

Ratio Type C-reactive Protein Immunosensor Based on Gold Nanoparticles/Fe-MIL-88-NH₂