Citation: ZHU Yong-Kang, MA Dan-Ying, LU Yan-Fei, SHANG Lin-Wei, ZHAO Yuan, ZHANG Zi-Wei, YIN Jian-Hua. Study on Synovial Fluid of Osteoarthritis by Surface-Enhanced Raman Scattering Spectroscopy[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(4): 484-490. doi: 10.19756/j.issn.0253-3820.191581 shu

Study on Synovial Fluid of Osteoarthritis by Surface-Enhanced Raman Scattering Spectroscopy

Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China

Received Date: 8 October 2019
Revised Date: 23 December 2019

Fund Project: This work was supported by the Six Talent Peaks Project of Jiangsu Province (No. SWYY-034), the National Natural Science Foundation of China (No. 61378087) and the Fundamental Research Funds for the Central Universities of China (No. kfjj20180309).

As an important part of joint, synovial fluid (SF) plays an important role in joint movement, lubrication and protection. The changes in main components of SF (proteins, proteoglycans, etc.) indicate osteoarthritis (OA) development, which may be used as biomarkers for OA diagnosis. Since detection sensitivity of Raman scattering is limited, it's hard to realize micro/trace composition detection of SF. Due to high sensitivity and high specificity, surface-enhanced Raman scattering (SERS) has become a preferred option for micro/trace detection. In this study, diluted serum and hyaluronic acid (HA) were used to verify the SERS feasibility in SF detection by the self-made nano silver sol. The SF drawn from the knee joint with 8-week OA (early OA) was investigated by SERS technique, and it was found that the scattering intensities of the protein, polysaccharide and pyranose ring were obviously enhanced, which could be used for the micro detection of SF. By comparing SERS spectra of SFs of the early OA and the health, it was found that contents of polysaccharide HA, sulfated polysaccharide and pyranose increased in the OA, showing significant differences with those in the health (p<0.05). The method proposed here opened up a promising and novel way to early OA diagnosis and micro/trace detection of biological tissue fluids.
- Surface-enhanced Raman scattering,
- Synovial fluid,
- Polysaccharides,
- Osteoarthritis
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