Citation: ZHANG Fu-Li, QIU Quan-Fa, YANG Xiao-Ke, HUANG Wei-Hua. Real-time Electrochemical Detection of Retrograde Messengers in Synaptic Transmission[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(10): 1689-1694. doi: 10.19756/j.issn.0253-3820.191485 shu

Real-time Electrochemical Detection of Retrograde Messengers in Synaptic Transmission

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

Received Date: 8 August 2019
Revised Date: 16 August 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21725504, 21675121, 21721005).

Nervous system is a significant command system, and the communication between neurons depends on anterograde messengers such as neurotransmitters released from presynaptic neurons and retrograde messengers from postsynaptic cells. As an important kind of retrograde messengers, nitric oxide can regulate synaptic strength for different physiological needs. Whereas, most current researches focus on presynaptic exocytosis of neurotransmitters but ignore the detection of retrograde messengers. To monitor retrograde messenger nitric oxide released from postsynaptic neurons, we developed an electrochemical sensor with rapid response, high sensitivity and spatio-temporal resolution by modifying platinum nanoparticles on carbon fiber microelectrodes. The ability of hippocampal neurons to synthesize and secrete nitric oxide was firstly evidenced by successful detection of nitric oxide released from L-arginine-or glutamate-stimulated neurons. On this basis, the neuronal transmission was initiated by stimulating presynaptic neuron with high potassium solution, and the nitric oxide released from postsynaptic neurons was monitored in real time. The results demonstrated that synaptic transmission was accompanied with the release of retrograde messengers. This method has promising potential in exploring synaptic feedback regulation and plasticity in nervous system.
- Hippocampal neurons,
- Synaptic transmission,
- Retrograde messengers,
- Nitric oxide,
- Electrochemical detection
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