Citation: HOU Zhuang-Hao, ZHAN Liu-Juan, LUAN Mou-Jun, TIAN Shuang-Shuang, DAI Meng-Jie, HUANG Guang-Ming. Metabolites Analysis of Single Cell by Mass Spectrometry and Application in Single Neuron Metabolites Analysis[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(10): 1601-1611. doi: 10.19756/j.issn.0253-3820.191459 shu

Metabolites Analysis of Single Cell by Mass Spectrometry and Application in Single Neuron Metabolites Analysis

Department of Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China

Received Date: 29 July 2019
Revised Date: 30 August 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21775143, 21475121), the Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology (No. 2017FXCX003) and the Fundamental Research Funds for the Central Universities (No. WK6030000116).

Single-cell assays has emerged as a cutting-edge technique during the past decade. Despite several issues (including pL volume, extremely complicated intracellular fluid and maintaining cell viability during sampling) still need to be addressed, single-cell mass spectrometry for metabolites has achieved remarkable results recently. Various mass spectrometry-based approaches have enabled identification of vast cytoplasmic chemical constituents at single cell level, which greatly facilitate the single neuron analysis at different physiological conditions. In this review, we have concluded recent single-cell mass spectrometry investigations toward single neuron analysis, including single ovum/zygote, single cultured cell, single freshly isolated cell and single cell/neuron on tissue.
- Single neuron analysis,
- Metabolites analysis,
- Single cell mass spectrometry,
- Review
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
42. [42]
43. [43]
44. [44]
45. [45]
46. [46]
47. [47]
48. [48]
49. [49]
50. [50]
51. [51]
52. [52]
53. [53]
54. [54]
55. [55]
56. [56]
57. [57]
58. [58]
59. [59]
60. [60]
61. [61]
62. [62]
63. [63]
64. [64]
65. [65]
66. [66]
67. [67]
68. [68]
69. [69]
70. [70]
71. [71]
72. [72]
73. [73]
74. [74]
75. [75]
76. [76]
77. [77]
78. [78]
79. [79]
80. [80]
81. [81]
82. [82]
83. [83]
84. [84]
85. [85]
86. [86]
87. [87]
88. [88]
89. [89]
90. [90]
91. [91]
92. [92]
93. [93]
94. [94]
95. [95]
96. [96]
97. [97]
98. [98]
99. [99]
100. [100]
101. [101]
102. [102]
103. [103]
104. [104]
105. [105]
106. [106]
107. [107]
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