Citation: CHEN Li-Pin, ZHANG Xiao-Mei, HU Ling-Ping, BI Shi-Jie, LIN Li-Ming, LI Zhao-Jie, ZHANG Hong-Wei, XUE Chang-Hu. Analysis of Changes of Endogenous Peptides of Live Pacific Oysters at Different Circulation Stages Based on Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(12): 1893-1900. doi: 10.19756/j.issn.0253-3820.191444 shu

Analysis of Changes of Endogenous Peptides of Live Pacific Oysters at Different Circulation Stages Based on Mass Spectrometry

1.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
2.
Qingdao Customs Technology Center, Qingdao 266002, China

Received Date: 25 July 2019
Revised Date: 21 October 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31871867), the National Quality Supervision, Inspection and Quarantine General Administration Science and Technology Plan Project (No. 2016IK204) and the Earmarked Fund for Modern Agro-industry Technology Research System (No. CARS-49).

To simulate the life-saving circulation of Pacific oysters, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF) was used to analyze the changes of endogenous peptides of live Pacific oysters at different circulation stages. Chemometric method was used to screen the differential peptide markers at each stage of circulation to obtain a set of new indicators for evaluating the quality changes in the circulation of live Pacific oysters. According to the peptide histology technique, the endogenous peptide was extracted from samples at 7 key time points in the whole process of circulation of live Pacific oysters (cleaning, temporary purification and nourishing), and then separated and identified by UHPLC-Q-TOF to obtain the peptide group profile at each circulation stage. Multivariate statistical analysis such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used for data mining and screening for differential markers. The online amino acid sequence was used to identify the amino acid sequences of differential peptides. The results showed that 3, 10, and 8 potential peptide markers were screened in the cleaning, temporary purification, and water-free preservation phases, respectively. The potential marker peptide DYDPVDK was selected for synthesis and the multi-reaction monitoring (MRM) analysis method based on liquid chromatography-triple quadrupole mass spectrometry was used to verify the correctness of the differential marker peptide sequence and its applicability for routine liquid chromatography analysis. In this work, peptide analysis was used for the first time to characterize the endogenous peptides of live Pacific oysters at different circulation stages. The differential marker peptides in different circulation stages were selected to provide the basis for detection of circulation process of live shellfish.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
1. [1]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085
2. [2]
  Weitai Wu , Laiying Zhang , Yuan Chun , Liang Qiao , Bin Ren . Course Design of Chemical Measurement Experiments in Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 64-68. doi: 10.12461/PKU.DXHX202409031
3. [3]
  Tianyu Feng , Guifang Jia , Peng Zou , Jun Huang , Zhanxia Lü , Zhen Gao , Chu Wang . Construction of the Chemistry Biology Experiment Course in the Chemistry “101 Program”. University Chemistry, 2024, 39(10): 69-77. doi: 10.12461/PKU.DXHX202409002
4. [4]
  Laiying Zhang , Weitai Wu , Yiru Wang , Shunliu Deng , Zhaobin Chen , Jiajia Chen , Bin Ren . Practices for Improving the Course of Chemical Measurement Experiments in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 107-112. doi: 10.12461/PKU.DXHX202409032
5. [5]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
6. [6]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
7. [7]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
8. [8]
  Yan Su , Xiuyun Wang , Huimin Guo , Yanjuan Zhang , Xinwen Zhang , Yunting Shang , Wenfeng Jiang . To Cultivate Scientific Literacy by Learning, Thinking, Practicing and Understanding, To Utilize the “Smart Eye” Expertise by Integrating of Knowledge and Action: Ideological and Political Construction of Analytical Chemistry Experiment Course. University Chemistry, 2024, 39(2): 196-202. doi: 10.3866/PKU.DXHX202308003
9. [9]
  Qiying Xia , Guokui Liu , Yunzhi Li , Yaoyao Wei , Xia Leng , Guangli Zhou , Aixiang Wang , Congcong Mi , Dengxue Ma . Construction and Practice of “Teaching-Learning-Assessment Integration” Model Based on Outcome Orientation: Taking “Structural Chemistry” as an Example. University Chemistry, 2024, 39(10): 361-368. doi: 10.3866/PKU.DXHX202311007
10. [10]
  Hongwei Ma , Fang Zhang , Hui Ai , Niu Zhang , Shaochun Peng , Hui Li . Integrated Crystallographic Teaching with X-ray,TEM and STM. University Chemistry, 2024, 39(3): 5-17. doi: 10.3866/PKU.DXHX202308107
11. [11]
  Jiantao Zai , Hongjin Chen , Xiao Wei , Li Zhang , Li Ma , Xuefeng Qian . The Learning-Centered Problem-Oriented Experimental Teaching. University Chemistry, 2024, 39(4): 40-47. doi: 10.3866/PKU.DXHX202309023
12. [12]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
13. [13]
  Yuqiao Zhou , Weidi Cao , Shunxi Dong , Lili Lin , Xiaohua Liu . Study on the Teaching Reformation of Practical X-ray Crystallography. University Chemistry, 2024, 39(3): 23-28. doi: 10.3866/PKU.DXHX202303003
14. [14]
  Hongbo Zhang , Yihong Tang , Suxia Zhang , Yuanting Li . Electrochemical Monitoring of Photocatalytic Degradation of Phenol Pollutants: A Recommended Comprehensive Analytical Chemistry Experiment. University Chemistry, 2024, 39(6): 326-333. doi: 10.3866/PKU.DXHX202310013
15. [15]
  Zongpei Zhang , Yanyang Li , Yanan Si , Kai Li , Shuangquan Zang . Developing a Chemistry Experiment Center Employing a Multifaceted Approach to Serve High-Quality Laboratory Education. University Chemistry, 2024, 39(7): 13-19. doi: 10.12461/PKU.DXHX202404041
16. [16]
  Zhenming Xu , Mingbo Zheng , Zhenhui Liu , Duo Chen , Qingsheng Liu . Experimental Design of Project-Driven Teaching in Computational Materials Science: First-Principles Calculations of the LiFePO₄ Cathode Material for Lithium-Ion Batteries. University Chemistry, 2024, 39(4): 140-148. doi: 10.3866/PKU.DXHX202307022
17. [17]
  Jia Huo , Jia Li , Yongjun Li , Yuzhi Wang . Ideological and Political Design of Physical Chemistry Teaching: Chemical Potential of Any Component in an Ideal-Dilute Solution. University Chemistry, 2024, 39(2): 14-20. doi: 10.3866/PKU.DXHX202307075
18. [18]
  Pengcheng Yan , Peng Wang , Jing Huang , Zhao Mo , Li Xu , Yun Chen , Yu Zhang , Zhichong Qi , Hui Xu , Henan Li . Engineering Multiple Optimization Strategy on Bismuth Oxyhalide Photoactive Materials for Efficient Photoelectrochemical Applications. Acta Physico-Chimica Sinica, 2025, 41(2): 100014-. doi: 10.3866/PKU.WHXB202309047
19. [19]
  Feiya Cao , Qixin Wang , Pu Li , Zhirong Xing , Ziyu Song , Heng Zhang , Zhibin Zhou , Wenfang Feng . Magnesium-Ion Conducting Electrolyte Based on Grignard Reaction: Synthesis and Properties. University Chemistry, 2024, 39(3): 359-368. doi: 10.3866/PKU.DXHX202308094
20. [20]
  Houjin Li , Wenjian Lan . Name Reactions in University Organic Chemistry Laboratory. University Chemistry, 2024, 39(4): 268-279. doi: 10.3866/PKU.DXHX202310016

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(585)
HTML views(111)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142