Citation: QI Camaoji, SHA Zheng-Yue, LI Xian-Chan. Electrochemical Analysis of Single Neuronal Vesicles[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(10): 1502-1511. doi: 10.19756/j.issn.0253-3820.191443 shu

Electrochemical Analysis of Single Neuronal Vesicles

Center for Imaging and Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China

Received Date: 25 July 2019
Revised Date: 2 September 2019

Fund Project: This work was supported by the Construction Fee for Teachers in Central Colleges and Universities of China (No.103010190040100) and the Youth Excellent Academic Team Construction Project of Minzu University of China (No. 2019QNBJ02).

Brain science has attracted more and more attention in the world recently. The storage, release and their function of chemical transmitters are important in neural biology. As the main subcellular organelle for chemical transmitter storage and release, nerve vesicles have attracted much attention. Compared with common vesicle analysis techniques, electroanalytical chemistry has advantages in the analysis of single nerve vesicle due to its high time resolution and sensitivity, especially the use of micro and nano electrodes. In recent years, vesicle impact electrochemical cytometry (VIEC) and resistive-pulse method have been applied rapidly in the field of single vesicles analysis. VIEC can be used to quantitate the storage of neurotransmitters in single vesicles by breaking the vesicle membrane on the electrode surface through electroporation. Resistive-pulse method can be used to measure the size, surface charge, and other parameters or characteristics of vesicles by monitoring the changes of ion current caused by single vesicles passing through micro/nanopores. Focusing on the electro-analysis of single nerve vesicles, this paper mainly introduces the important progress of VIEC and resistive-pulse method in the field of single vesicles analysis in recent years, and has a briefly outlook of the application of these two methods in life analytical chemistry including neuro-analytical chemistry and biomedicine.
- Brain science,
- Nerve vesicles,
- Electroanalytical chemistry,
- Vesicle impact electrochemical cytometry,
- Resistive-pulse method,
- Review
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
38. [38]
39. [39]
40. [40]
41. [41]
42. [42]
43. [43]
44. [44]
45. [45]
46. [46]
47. [47]
48. [48]
49. [49]
50. [50]
51. [51]
1. [1]
  Kun Li , Na Gao , Shuangyan Huan , Yuzhi Wang . Design of Ideological and Political Education for the Experiment of Detecting Cadmium with Anodic Stripping Voltammetry. University Chemistry, 2024, 39(2): 155-161. doi: 10.3866/PKU.DXHX202307068
2. [2]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
3. [3]
  Yan Su , Xiuyun Wang , Huimin Guo , Yanjuan Zhang , Xinwen Zhang , Yunting Shang , Wenfeng Jiang . To Cultivate Scientific Literacy by Learning, Thinking, Practicing and Understanding, To Utilize the “Smart Eye” Expertise by Integrating of Knowledge and Action: Ideological and Political Construction of Analytical Chemistry Experiment Course. University Chemistry, 2024, 39(2): 196-202. doi: 10.3866/PKU.DXHX202308003
4. [4]
  Zhuo Wang , Xue Bai , Kexin Zhang , Hongzhi Wang , Jiabao Dong , Yuan Gao , Bin Zhao . MOF模板法合成氮掺杂碳材料用于增强电化学钠离子储存和去除. Acta Physico-Chimica Sinica, 2025, 41(3): 2405002-. doi: 10.3866/PKU.WHXB202405002
5. [5]
  Yongming Zhu , Huili Hu , Yuanchun Yu , Xudong Li , Peng Gao . Construction and Practice on New Form Stereoscopic Textbook of Electrochemistry for Energy Storage Science and Engineering: Taking Basic Course of Electrochemistry as an Example. University Chemistry, 2024, 39(8): 44-47. doi: 10.3866/PKU.DXHX202312086
6. [6]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089
7. [7]
  Yan Zhang , Ping Wang , Tiebo Xiao , Futing Zi , Yunlong Chen . Measures for Ideological and Political Construction in Analytical Chemistry Curriculum. University Chemistry, 2024, 39(4): 255-260. doi: 10.3866/PKU.DXHX202401017
8. [8]
  Zhenli Sun , Ning Wang , Kexin Lin , Qin Dai , Yufei Zhou , Dandan Cao , Yanfeng Dang . Visual Analysis of Hotspots and Development Trends in Analytical Chemistry Education Reform. University Chemistry, 2024, 39(11): 57-64. doi: 10.12461/PKU.DXHX202403095
9. [9]
  Hongbo Zhang , Yihong Tang , Suxia Zhang , Yuanting Li . Electrochemical Monitoring of Photocatalytic Degradation of Phenol Pollutants: A Recommended Comprehensive Analytical Chemistry Experiment. University Chemistry, 2024, 39(6): 326-333. doi: 10.3866/PKU.DXHX202310013
10. [10]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
11. [11]
  Liuchuang Zhao , Wenbo Chen , Leqian Hu . Discussion on Improvement of Teaching Contents about Common Evaluation Parameters in Analytical Chemistry. University Chemistry, 2024, 39(2): 379-391. doi: 10.3866/PKU.DXHX202308079
12. [12]
  Guangming Yang , Yunhui Long . Design and Implementation of Analytical Chemistry Curriculum Based on the Learning Community of Teachers and Students. University Chemistry, 2024, 39(3): 132-137. doi: 10.3866/PKU.DXHX202309089
13. [13]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
14. [14]
  Tian Li , Liping Zhang , Ling Liu , Ruifang Li , Longfei Mao , Hui Yang . Reform and Practice of Analytical Chemistry Teaching under the Guidance of Course Ideology and Politics. University Chemistry, 2024, 39(6): 189-194. doi: 10.3866/PKU.DXHX202310014
15. [15]
  Fan Yu , Aihua Li , Yun Liu , Tianrong Zhu , Liang Wang , Junhui Xu , Yazhen Wang . Exploration and Practice in Developing a Premier Course in Inorganic and Analytical Chemistry. University Chemistry, 2024, 39(8): 36-43. doi: 10.3866/PKU.DXHX202312037
16. [16]
  Shuangyan Huan , Jianhui Jiang , Gongke Li , Wenqing Zhang , Na Li , Yi Yang , Bin Hu , Yang Tian , Shuo Wu , Sichun Zhang , Yonghai Yue , Dechen Jiang , Zengping Chen , Zhonglin Lu , Cheng Cui , Yuzhi Wang , Weihong Tan . Exploration on the Construction of Analytical Chemistry Core Curriculum in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 22-26. doi: 10.12461/PKU.DXHX202409003
17. [17]
  Yaqian Duan , Juan Su , Meiyu Lin , Yuxin Fang , Wenyi Liang . Exploration of the Implementation Path of Ideological and Political Education in the “Dual-Track Teaching” Model: a Case Study of Analytical Chemistry Experiment. University Chemistry, 2024, 39(2): 181-188. doi: 10.3866/PKU.DXHX202307024
18. [18]
  Sheng Zhang , Mingyu Wang , Xiaohong Wang , Jiancheng Feng . Multidimensional Teaching Design and Ideological and Political Exploration of Analytical Chemistry Experiment under the Complete Credit System. University Chemistry, 2024, 39(2): 189-195. doi: 10.3866/PKU.DXHX202307071
19. [19]
  Ling Bai , Limin Lu , Xiaoqiang Wang , Dongping Wu , Yansha Gao . Exploration and Practice of Teaching Reforms in “Quantitative Analytical Chemistry” under the Perspective of New Agricultural Science. University Chemistry, 2024, 39(3): 158-166. doi: 10.3866/PKU.DXHX202308101
20. [20]
  Hongyi Zhang , Zhihong Shi , Zhijun Zhang . A New Strategy for “De-formulized” Calculation of Dynamic Buffer Capacity in Analytical Chemistry Education. University Chemistry, 2024, 39(3): 390-394. doi: 10.3866/PKU.DXHX202309030

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(1004)
HTML views(142)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142