Citation: CHEN Wei, LEI He-Hua, SONG Tao, ZHANG Li-Min, LEI Hao. Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo ¹H-MRS and Comparison of Detection Results of Tissue Extracts Obtained by ¹H-NMR and UHPLC-MS/MS[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(10): 1671-1679. doi: 10.19756/j.issn.0253-3820.191439 shu

Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo ¹H-MRS and Comparison of Detection Results of Tissue Extracts Obtained by ¹H-NMR and UHPLC-MS/MS

CHEN Wei ^1,2, ,
LEI He-Hua ¹ ,
SONG Tao ¹ ,
ZHANG Li-Min ¹ ,
LEI Hao ¹

1.
National Center for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 24 July 2019
Revised Date: 28 August 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos.21790390, 21790392).

In vivo proton magnetic resonance spectroscopy (¹H-MRS) can be used to measure regional concentration of multiple brain metabolites simultaneously and non-invasively. The technique has been widely used in the clinical diagnosis and basic researches on neurological/psychiatry diseases. In this study, the concentrations of five cerebral metabolites, namely N-acetyl aspartate (NAA), glutamate (Glu), glutamine (Gln), taurine (Tau) and glutathione (GSH) in two brain regions (i.e., striatum and medial prefrontal cortex) of 12-months old mice were measured by in vivo ¹H-MRS using water signal as the internal standard. The detection results were then compared with those obtained by liquid-state ¹H-NMR and ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The results demonstrated that the NAA, Glu and Tau concentrations measured by the three techniques had no statistically significant differences among each other, suggesting that the results of in vivo ¹H-MRS analyses were at least as reliably and accurate as those obtained by ¹H-NMR and UHPLC-MS/MS. The absolute concentrations of Gln and GSH measured by UHPLC-MS/MS were significantly higher or lower, relative to those measured with magnetic resonance techniques. Such discrepancies might have originated from the systematic errors presented in the UHPLC-MS/MS measurements of Gln and GSH. The results demonstrated the feasibility of combined or complementary use of in vivo ¹H-MRS and ex vivo measurements for metabolite profiling in the brain.
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Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo 1H-MRS and Comparison of Detection Results of Tissue Extracts Obtained by 1H-NMR and UHPLC-MS/MS

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通讯作者: 陈斌, bchen63@163.com

Quantitative Analysis of Cerebral Metabolites in Mice by in Vivo ¹H-MRS and Comparison of Detection Results of Tissue Extracts Obtained by ¹H-NMR and UHPLC-MS/MS