Citation: XIAO Kun, GONG Can, GUO Qiang-Sheng, XU Xu. Simultaneous Determination of Fatty Acids at sn-1,3 and sn-2 of Triglyceride in Edible Oils by Quantitative ¹³C-Nuclear Magnetic Resonance[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(6): 802-810. doi: 10.19756/j.issn.0253-3820.191420 shu

Simultaneous Determination of Fatty Acids at sn-1,3 and sn-2 of Triglyceride in Edible Oils by Quantitative ¹³C-Nuclear Magnetic Resonance

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Received Date: 19 July 2019
Revised Date: 6 March 2020

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 31671928) and the Natural Science Foundation of Shanghai, China (No. 15ZR1440800).

A ¹³C-quantitative nuclear magnetic resonance (qNMR) method was established for determination of content of fatty acid at different positions in edible oil. The positional specificity analysis of sn-1,3 and sn-2 in fatty acids in edible oil was performed using reverse-gated decoupling technique. The direct normal integral, deconvolution integral using different Lorentz-Gaussian ratios, and other data processing methods were compared for the analyzing ¹³C-qNMR unseparated peaks. Then the deconvolution integral with Lorentz-Gauss (3∶2) was selected to extract the quantitative peak areas of two unsaturated fatty acids at sn-1,3 and sn-2, linoleic acid and oleic acid. The content of unsaturated fatty acids in different positions showed that the saturated fatty acids, sn-1 linoleic acid, sn-2 linoleic acid, sn-1,3 oleic acid, sn-2 oleic acid were as follows: soybean oil (16.2%, 27.8%, 24.0%, 15.5%, 7.9% (w/w, the same below)); corn oil (15.3%, 30.7%, 20.5%, 20.1%, 13.3%); peanut oil (18.3%, 18.7%, 12.5%, 24.9%, 25.5%). The sn-1,3 linolenic acid, sn-2 linolenic acid in soybean oil were 4.5% and 4.1%, respectively, but were undetectable in corn oil and peanut oil. The result was in accordance with content of total linoleic acid and total oleic acid detected by above ¹H-qNMR method. ¹³C-qNMR can be applied to distinguish the location and distribution of unsaturated fatty acids with easy sample preparation and flexible operation, providing a method for quantifying the specific triglyceride positional isomer component in edible oil without its standard sample.
- Quantitative nuclear magnetic resonance,
- ¹³C-nuclear magnetic resonance,
- Edible oil,
- Fatty acid,
- Positional isomer
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Simultaneous Determination of Fatty Acids at sn-1,3 and sn-2 of Triglyceride in Edible Oils by Quantitative ¹³C-Nuclear Magnetic Resonance