Citation: WANG Shuai, QIAO Yi-Piao, HUANG Xuan-Yun, LI Xue-Hui, HUANG Dong-Mei, TIAN Liang-Liang, FANG Chang-Ling, YE Hong-Li, SHI Yong-Fu. Identification of Metabolites of 2,2',4,5,5'-Pentachlorodiphenyl in Mice[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(12): 1951-1959. doi: 10.19756/j.issn.0253-3820.191259 shu

Identification of Metabolites of 2,2',4,5,5'-Pentachlorodiphenyl in Mice

1.
Fishery Products Quality Inspection and Test Centre(Shanghai), Ministry of Agriculture and Rural Affairs of China, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
2.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
3.
Agricultural Products Quality and Safety Inspection Station of Linquan County, Anhui province, Linquan 236400, China;
4.
Shanghai Institute of Measurement and Testing Technology, Shanghai 200233, China

Received Date: 6 May 2019
Revised Date: 14 October 2019

Fund Project: This work was supported by the Agriculture Industry Research Special Funds for Public Welfare Projects (No. 201503108) and the National Natural Science Foundation of China (No. 31501572).

In this study, metabolites of 2,2',4,5,5'-pentachlorodiphenyl (PCB101) in mice were identified. Kunming mice were exposed to PCB101 by intraperitoneal injection, and gas chromatography-tandem mass spectrometry (GC-MS/MS) was adopted for metabolites identification. It was confirmed that 5 kinds of compounds including 2 hydroxylated-PCB101, 2 methyl sulfonated-PCB101 and 1 methoxylated-PCB101 were metabolized. The hydroxylated metabolites were identified as 3-OH-PCB101 and 4-OH-PCB101 mainly in the stomach, intestines, gonads, liver and blood of mice. Hydroxylation might occur in the 2,5-dichlorinated ring, via formation of an 3,4-arene oxide that subsequently rearranged to a hydroxyl group by epoxide opening, or by direct insertion of a hydroxyl group in non-chlorinated substitution site. Methyl sulfonated metabolites were identified as 3-MeSO₂-PCB101 and 4-MeSO₂-PCB101 mainly in the stomach, intestines, kidneys, gonads, liver and lungs of mice, methyl sulfonation might also involve the formation of 3,4-epoxides, and then underwent the mercapturic acid pathway. Methoxylated metabolite was identified as 4-MeO-PCB101 mainly in the intestines, kidneys, lungs, muscles, spleen and brain of mice, simultaneously. In this study, it was basically confirmed that PCB101 could be biotransformed into three types of metabolites in mice at least, and methoxylated metabolite was confirmed in mice for the first time, which was of great scientific significance for further study on the persistence, toxicity effects and risk assessment of PCB metabolites in organisms.
- Polychlorinated biphenyls,
- Hydroxylated,
- Methyl sulfonated,
- Methoxylated,
- Metabolites,
- Identification,
- Gas chromatography-tandem mass spectrometry
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沈阳化工大学材料科学与工程学院沈阳 110142