Citation: LI Peng-Wei, ZHANG Yao, LI Qing-Lian, GAO Pan, JIA Neng-Qin. Three-Dimensional Graphene/Prussian Blue-based Electrochemical Enzyme Sensor for Sensitive Determination of Uric Acid[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(12): 1938-1945. doi: 10.19756/j.issn.0253-3820.191243 shu

Three-Dimensional Graphene/Prussian Blue-based Electrochemical Enzyme Sensor for Sensitive Determination of Uric Acid

The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China

Received Date: 30 April 2019
Revised Date: 10 July 2019

Fund Project: This work was supported by the Shanghai Science and Technology Committee (Nos. 17070503000, 18dz2308700) and the Shanghai Engineering Research Center of Green Energy.

A three-dimensional reduced graphene oxide (3D GR)-Prussian blue (PB)-based electrochemical enzyme sensor was constructed for sensitive detection of uric acid. The graphene oxide was thermally reduced by one-step hydrothermal method, and then 3D GR was formed by self-assembly. PB was electrodeposited onto 3D GR to synthesize 3D GR-PB composite. Due to its large specific surface area and porous nature, 3D GR provided more active sites for the reaction. Electrochemical tests showed that the enzyme sensor based on 3D GR-PB had a wide linear range (3.75×10^-9-3.75×10^-4 mol/L) and a low detection limit (4.21×10^-10 mol/L). Because of the high specificity of uricase to catalyze uric acid, this sensor had high selectivity and provided a feasible solution for sensitive detection of uric acid.
- Three-dimensional reduced graphene oxide,
- Prussian blue,
- Uric acid,
- Electrochemical enzyme sensor
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