Citation: FANG Han-Shu, LANG Ming-Fei, SUN Jing. New Methods for Cell Cycle Analysis[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(9): 1293-1301. doi: 10.19756/j.issn.0253-3820.191225
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Cell cycle is a basic process during the cell life. Analyzing cell cycles can better understand the nature of diseases. Currently, cell cycles are mainly analyzed by flow cytometry, which investigate cell cycles by measuring intracellular DNA contents and cell cycle specific proteins. With the development of technology, several new methods have emerged for cell cycle analysis in recent years to improve and enhance image analysis, single-cell analysis, sample reduction, and high throughput. These new methods including image flow cytometry, mass flow cytometry, microfluidic technology and Raman spectroscopy greatly expand the methods for cell cycle analysis and increase the accuracy of cell cycle measurements. Here we briefly introduced these new methods for cell cycle analysis.
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Keywords:
- Cell cycle,
- Cell cycle proteins,
- DNA contents,
- Microfluidics,
- Flow cytometry,
- Review
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