Citation: WANG Xue-Jing, MU Wei, HAN Xiao-Jun. Preparation Methods for Phospholipid Vesicles Arrays and Their Applications in Biological Analysis[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(8): 1134-1144. doi: 10.19756/j.issn.0253-3820.191199 shu

Preparation Methods for Phospholipid Vesicles Arrays and Their Applications in Biological Analysis

State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China

Received Date: 11 April 2019
Revised Date: 28 May 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21773050), the Natural Science Foundation of Heilongjiang Province for Distinguished Young Scholars (No. JC2018003).

Lipid vesicles, composed of cell membrane components, are commonly used as an artificial cell model to mimic cell structure and functions. They have also been widely used in biological analysis. Liposome array is a high throughput platform for protein screening and bio-sensing. This review focuses on the preparation methods of homogeneous small lipid vesicle array, heterogeneous small lipid vesicle array, and giant lipid vesicle array, as well as their applications in antibiotics detection, enzymatic reactions, interactions between membrane proteins and lipid membranes. Besides, the advantages and disadvantages of the reported methods are summarized and the future directions in this field are proposed.
- Homogeneous small lipid vesicle array,
- Heterogeneous small lipid vesicle array,
- Giant lipid vesicle array,
- Artificial cell,
- Biological analysis,
- Review
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