Citation: LI Xin, LI Jia-Qi, JIN Gao-Wa, YU Long, GUO Zhi-Mou, LIANG Xin-Miao, LIU Shuai, YAN Jing-Yu. Structural Analysis of High Degree Polymerization Isomers of Human Milk Oligosaccharides Based on Electrospray Ionization- Quadrupole-Time of Flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(7): 1106-1113. doi: 10.19756/j.issn.0253-3820.191167 shu

Structural Analysis of High Degree Polymerization Isomers of Human Milk Oligosaccharides Based on Electrospray Ionization- Quadrupole-Time of Flight Mass Spectrometry

LI Xin ^1,2, ,
LI Jia-Qi ² ,
JIN Gao-Wa ² ,
YU Long ² ,
GUO Zhi-Mou ² ,
LIANG Xin-Miao ² ,
LIU Shuai ¹ ,
YAN Jing-Yu ²

1.
Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China;
2.
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Received Date: 28 March 2019
Revised Date: 25 April 2019

Fund Project: This work was supported by the National Key Research and Development Program of China (Nos.2017YFD0400601,2016YFB0301502) and the National Natural Science Foundation of China (No.U1608255).

Human milk oligosaccharides are a kind of natural prebiotics that exist in human milk. They play an irreplaceable role in the growth of infantile intestinal flora, the improvement of immune system and the resistance to pathogen infection. The function of human milk oligosaccharides is closely related to their structures which are complex with various linkages. Besides, the oligosaccharides have a large number of isomers, the number and complexity of which increase dramatically with the degrees of their polymerization. Therefore, the structural analysis of oligosaccharide isomers with a high degree of polymerization is one of the difficulties in the study of human milk oligosaccharides, and is of great significance for the study of the biological function of oligosaccharides. In this study, electrospray ionization-quadrupole-time of flight mass spectrometry (ESI-Q/TOF-MS) was used to analyze the structures of eighteen high-degree polymerized human milk oligosaccharides in a negative ion mode, and three of them were firstly reported. Differences of fragment ions between the isomers were discussed, and the cleavage rules of oligosaccharide isomers were summarized. This study provided a scientific basis for the structural analysis of the complex isomers of highly polymerized human milk oligosaccharides and the discovery of novel sugar structures.
- Human milk oligosaccharides,
- Isomers,
- Structural analysis,
- Electrospray ionization-quadrupole-time of flight mass spectrometry
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