Citation: CAI Fang, REN Fan-Dong, REN Da-Bing, LIU Shao, YI Lun-Zhao. Integrated Qualitative Strategy for High-throughput Targeted Filtering of Emodin Metabolites[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(8): 1212-1218. doi: 10.19756/j.issn.0253-3820.191118 shu

Integrated Qualitative Strategy for High-throughput Targeted Filtering of Emodin Metabolites

1.
Food Safety Research Institute, Kunming University of Science and Technology, Kunming 650504, China;
2.
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China

Received Date: 4 March 2019
Revised Date: 21 May 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21775058).

The emodin and its metabolites in serum of renal interstitial fibrosis rats were separated on a Thermo Scientific Hypersil GLOD column with the mixture of 0.1% formic acid water and acetonitrile as the mobile phase, and then analyzed by ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS). In this study, an integrated qualitative strategy was proposed to identify the target metabolites of active pharmaceutical ingredients in vivo, which combined mass defect, neutral loss, and diagnostic fragment ion filtering. The method was applied to the identification of metabolites of emodin in rat blood, and 11 metabolites were determined accurately, 39 possible metabolites were tentatively identified. The result shows that the metabolism pathways of emodin in rats were mainly oxidation and hydroxylation in phase Ι, and sulfation and glucuronidation in phase Ⅱ. The integrated qualitative strategy proposed here could effectively reduce matrix interference and interfering ions in the qualitative process, thus improveing the efficiency and accuracy of qualitative analysis of metabolites.
- Ultra performance liquid chromatography-high-resolution mass spectrometry,
- Mass defect,
- Diagnostic fragment ion,
- Neutral loss,
- Emodin
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