Citation: ZHAO Ying, WANG Shuang-Jie, LIU Ying, JIAO Sha-Sha, ZOU Ru-Bing, FANG Yi-Hua, GUO Yi-Rong, ZHU Guo-Nian. Immunochip Assay for Multi-residue Rapid Detection of Ten Kinds of Common Pesticides[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(11): 1759-1766. doi: 10.19756/j.issn.0253-3820.191068 shu

Immunochip Assay for Multi-residue Rapid Detection of Ten Kinds of Common Pesticides

Institute of Pesticide and Environmental Toxicology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou 310029, China

Received Date: 30 January 2019
Revised Date: 24 September 2019

Fund Project: This work was supported by the National Key Research and Development Program of China (Nos. 2017YFF0210200, 2016YFD0201302), the Zhejiang Pbulic Welfare Technolgoy Application Research Project (No. 2016c32004), and the National Natural Science Foundation of China (No. 31871994).

With aldehyde-modified glass slide as solid phase, pesticide competitive antigens as coating antigens, gold nanoparticles as labeling material, anti-pesticide monoclonal antibodies as recognition elements and silver-enhance reagent as signal amplification, an immunochip containing 10 combinations of coating antigens and antibodies against corresponding pesticides was developed, which could be used for simultaneous detection of 10 kinds of pesticide residues in agricultural products, including chlorpyrifos, triazophos, carbofuran, thiazoline, imidacloprid, carbendazim, iprodione, aldicarb, fenpropathrin and chlorothalonil. The limits of detection (IC₂₀) of 10 kinds of pesticides were 1.49-15.72 μg/L under the optimized reaction conditions of immunochip. Moreover, the detection process took only 1.5 h. The chip method was applied to the detection of 10 kinds of pesticide residues in fruits (apples), vegetables (cucumbers) and tea (black tea). The sensitivity of the chip met the national Maximum Residue Limit (MRL) standard of pesticides on corresponding crops. Furthermore, the immunochip assay had high accuracy and precision, with recoveries of pesticides from 82.1% to 120.8%, and intra-day and inter-day coefficient of variation less than 10.4% and 12.1%, respectively. The results from the immunochip assay had good correlation with those from mass spectrometer, with R² of over 0.9591. This immunochip assay provided technical support for rapid detection of pesticide residues, and had practical significance for guaranteeing the quality and safety of agricultural products.
- Multiple pesticide residues,
- Rapid detection,
- Immunochip,
- Gold nanoparticles,
- Competitive immunoreaction
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