Citation: CAO Jin-Jin, SUN Qi-Rui, LI Wen-Hong, SONG Ran-Ran, CAO Qian-Yu, WANG Ke, WEI Yong-Ju. Fluorescence Properties of Magnoflorine and Its Application in Analysis of Traditional Chinese Medicine[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(6): 950-956. doi: 10.19756/j.issn.0253-3820.191043 shu

Fluorescence Properties of Magnoflorine and Its Application in Analysis of Traditional Chinese Medicine

CAO Jin-Jin ^1,2, ,
SUN Qi-Rui ² ,
LI Wen-Hong ³ ,
SONG Ran-Ran ² ,
CAO Qian-Yu ^2,4 ,
WANG Ke ⁴ ,
WEI Yong-Ju ^1,2

1.
College of Life Science, Hebei Normal University, Shijiazhuang 050024, China;
2.
College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang 050024, China;
3.
Department of Environmental and Chemical Engineering, Hebei College of Industry and Technology, Shijiazhuang 050091, China;
4.
Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050011, China

Received Date: 20 January 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 20975029,81173496) and the Natural Science Foundation of Hebei Province, China (No. B2014205129)

Fluorescence and absorption spectra of magnoflorine (MAG) were studied. The effects of environmental factors such as pH on fluorescence and absorbance were investigated, and the relationship between spectral properties and molecular structure was revealed. Three fluorescent peaks appeared in the three-dimensional (3D) fluorescence spectra of the aqueous solution of MAG, with excitation wavelengths (λ_ex) of 230, 275 and 315 nm, and the same emission wavelength (λ_em) of 420 nm. As the pH of the solution increased, the fluorescence peak in the excitation spectrum red-shifted and an equal fluorescence point appeared, and the absorption peak in the absorption spectrum also red-shifted and an isosbestic point formed, indicating that proton ionization occurred in one hydroxyl group in the MAG molecule. The proton ionization constant (pK_a=4.77) of MAG was determined by a pH-absorption method. Using L-tryptophan as a reference, the fluorescence quantum yield of the aqueous solution of MAG was determined to be Y=0.19. The characteristic fluorescence peaks of MAG appeared in the 3D fluorescence spectra of various Chinese herbal medicines. Using methanol-water mixed solvent containing 60% methanol, the extract of Sinomenium acutum (Qingfengteng) was prepared and diluted with water, then 3D fluorescence spectra were obtained. It was observed that the fluorescence peak at λ_ex/λ_em=315 nm/420 nm in the spectrum of the Qingfengteng neutral aqueous solution was not affected by the coexisting components. Based on this, a method for the determination of MAG in Qingfengteng was established. In the range of 0.04-1.25 μg/mL, the regression equation for fluorescence intensity (I_F) and MAG concentration (c) was I_F=6146.8c + 24.4, (R=0.999, n=11), with a detection limit of 0.52 ng/mL. The content of MAG in the Qingfengteng reference material was determined to be 0.63% by the method, and the recoveries were 101.2%-102.7%. The content of MAG (0.61%) in the same sample was also determined by LC-MS/MS method, which was consistent with the fluorescence method.
- Magnoflorine,
- Sinomenium acutum (Qingfengteng),
- Chinese medicine,
- Three-dimensional fluorescence,
- Fluorescence analysis
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