Citation: CHEN Jia-Qi, WU Hai-Yan, ZHANG Xu-Zhi, ZHENG Guan-Chao, SUN Xiao-Jie, GUO Meng-Meng, TAN Zhi-Jun, ZHAI Yu-Xiu, MU Hai-Jin. A Disposable Aptasensor for Sensitive Detection of Okadaic Acid in Shellfish[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(6): 869-875. doi: 10.19756/j.issn.0253-3820.191014 shu

A Disposable Aptasensor for Sensitive Detection of Okadaic Acid in Shellfish

1.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

Received Date: 7 January 2019

Fund Project: This work was supported by the National Key R&D Program of China (No. 2017YFC1600701), the National Natural Science Foundation of China (No. 31772075) and the Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS (No. 20603022016007).

Based on the specific combination between aptamer and its target analyte, a disposable aptasensor was built for the sensitive detection of okadaic acid (OA) (a main constituent of diarrhetic shellfish poisoning) in shellfish. The aptamer was employed as the probe and screen-printed gold electrodes (SPGE) as the support. Electrochemical impedance spectroscopy (EIS) was performed to test the change of electron transfer resistances (R_et) of SPGE before and after binding with OA and OA in shellfish samples could be thus quantified. In order to improve the sensitivity of the method, Fe₃O₄@Au was introduced into the fabrication of SPGE since it could enhance the gold working electrode's surface areas. Compared with antibody, the use of aptamer was cheaper, and SPGE avoided the polish step which was needed of traditional electrodes. The change of Ret was linearly correlated with the natural logarithm of concentration of OA from 1 μg/kg to 800 μg/kg. The limit of detection was 1 μg/kg. Recoveries in diarrhetic shellfish at different spiked levels were in the range of 92.0%-118.6%, and relative standard deviations (RSDs) were less than 12%. The proposed disposable aptasensor was efficient and sensitive with good repeatability, and was suitable for screening and quantification of shellfish samples.
- Okadaic acid,
- Aptasensor,
- Electrochemical impedance spectroscopy,
- Screen printed gold electrode,
- Sensitive detection
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沈阳化工大学材料科学与工程学院沈阳 110142