Citation: WEN Hui, LIU Xun, ZHANG Shang-Qing, YANG Ting, CHEN Ming-Li, WANG Jian-Hua. Discussion on Definition of Detection Limit for Ratio Fluorescence pH Sensing Methods[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(4): 597-604. doi: 10.19756/j.issn.0253-3820.181765
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The detection limit is an important parameter for evaluating the sensitivity of an analytical method. Compared with normally fluorescence probes, the calculation of detection limit of ratio fluorescence probes is more complicated. When the ratio fluorescence probe is used for pH detection, there is no universal definition of detection limit. The ratio fluorescence probes are classified into three categories based on the data types. A simulating probe with an excitation wavelength of 365 nm and an emission wavelength of 600 nm/450 nm is fabricated. Based on the calculation of detection limit for spectral method, a reasonable process is proposed for definition of detection limit for ratio fluorescence probe, which paves the way for comparing the sensitivity of different kinds of pH ratio fluorescence probes.
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Keywords:
- Ratio fluorescent probe,
- pH sensing,
- Sensitivity,
- Simulation data
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