Citation: WEN Hui-Zhong, LIANG Ying-Cai, XIAO Cheng-Yue, QIU Zi-Qing, LI Juan, LI Yan-Yan, ZHONG Qing-Mei, SHU Xin, WANG Yi-Lin. Synthesis of Catalytically Active Carbon Quantum Dots and Its Application to Colorimetric Detection of Cysteine[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(4): 591-596. doi: 10.19756/j.issn.0253-3820.181541 shu

Synthesis of Catalytically Active Carbon Quantum Dots and Its Application to Colorimetric Detection of Cysteine

School of Chemistry and Chemical Engineering, Guangxi University, Guangxi Key Laboratory of Biorefinery, Nanning 530004, China

Received Date: 19 August 2018
Revised Date: 18 January 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21661006), the Opening Project of Guangxi Key Laboratory of Biorefinery (No. GXKLB-201801) and the Foundation of College Student Experimental Skills and Innovation Ability Training of Guangxi University, China (No. 201810593040).

Carbon quantum dots (CQDs) were prepared from litchi rind. CQDs could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to form oxidized TMB (ox-TMB) with an absorption peak at 652 nm. However, the introduction of cysteine (CySH) could cause the reduction of ox-TMB, resulting in a decrease of the absorbance at 652 nm. Based on this, a colorimetric method using CQDs as mimetic peroxidase was developed for determination of CySH. With TMB as a substrate, the effect of experiment conditions including pH of system and reaction temperature on catalytic activity of the obtained CQDs was studied. Under the optimal conditions, e.g. pH 3.5, reaction temperature of 40℃, reaction time of 10 min, 0.5 mmol/L H₂O₂, 0.5 μg/mL CQDs and 0.25 mmol/L TMB, the response was linearly proportional to the concentration of CySH in the range of 0.05-4.00 μmol/L with a detection limit of 0.02 μmol/L(3σ/k). The method proposed here exhibited excellent selectivity and common amino acids in serum did not interfere with detection of CySH. This method was successfully applied to determination of CySH in human serum sample with recoveries of 94.0%-104.0%.
- Carbon quantum dots,
- Mimetic enzyme,
- Cysteine
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