分散固相萃取-液相色谱-三重四级杆质谱法分析水样中卤代醌类化合物

引用本文: 钟燕辉, 王冉, 林子俺. 分散固相萃取-液相色谱-三重四级杆质谱法分析水样中卤代醌类化合物[J]. 大学化学, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017 shu

Citation: Yanhui Zhong, Ran Wang, Zian Lin. Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry[J]. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017 shu

分散固相萃取-液相色谱-三重四级杆质谱法分析水样中卤代醌类化合物

福州大学化学学院, 福州 350108
基金项目:
国家自然科学基金(22274021,22036001)；福建省自然科学基金(2022J01535)；福建省本科高校教育教学研究项目(FBJG20220268)；福州大学本科教育教学研究项目

摘要: 本实验旨在通过分散固相萃取-液相色谱-三重四级杆质谱法分析环境水样中的卤代醌类化合物。实验选取了5种卤代醌类化合物作为目标物，采用阳离子共价有机骨架材料作为固相吸附剂，从水样中富集目标物。随后，使用液相色谱-三重四级杆质谱法对富集的化合物进行定性和定量分析。实验考察了吸附剂用量、富集时间、洗脱时间、洗脱溶剂种类、洗脱溶剂酸度和洗脱体积等条件对实验结果的影响，并建立了一种基于分散固相萃取的新方法。该方法在0.1-100 ng·L^-1范围内显示出较好的线性关系(相关系数R² ≥ 0.9924)，检出限可达到0.03-0.65 ng·L^-1，并成功应用于分析自来水样品中卤代苯醌类化合物。通过这个实验，学生可以了解基本的样品前处理技术，并提高实验技能、研究和创新思维，增强解决问题的能力。

关键词:

English

Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry

College of Chemistry, Fuzhou University, Fuzhou 350108, China
Received Date: 03 February 2024
Revised Date: 15 April 2024
Available Online: 29 July 2024

Abstract: This experiment aims to analyze halogenated quinone compounds in environmental water samples using dispersive solid-phase extraction (dSPE) combined with liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS). Five types of halogenated quinone compounds were used as analytes, and a cationic covalent organic framework material was employed as the solid-phase adsorbent to concentrate the target compounds from the water samples. Subsequently, the enriched compounds were subjected to qualitative and quantitative analysis by using LC-MS/MS. The experiment systematically examined the impact of various parameters on the experimental results, including the amount of the adsorbent, enrichment time, elution time, types of elution solvent, acidity of the elution solvent, and elution volume. A novel approach based on dSPE was developed. The method exhibited favorable linearity (with a correlation coefficient of R² ≥ 0.9924) in the range of 0.1-100 ng·L^-1, with detection limits ranging from 0.03-0.65 ng·L^-1. The method was successfully applied to the analysis of halogenated quinone compounds in tap water samples. This experiment helps students understand fundamental sample preparation techniques and enhances their practical skills, research capabilities, and problem-solving abilities.

Key words:

Dispersive solid-phase extraction
/ Liquid chromatography coupled-triple quadrupole mass spectrometry
/ Halogenated quinone compounds
/ Water

1. [1]
  Kim, L.; Lee, D.; Cho, H. K.; Choi, S. D. Trends Environ. Anal. 2019, 22, e00063.Kim, L.; Lee, D.; Cho, H. K.; Choi, S. D. Trends Environ. Anal. 2019, 22, e00063.
2. [2]
  Gao, M. J.; Fu, Q. F.; Wang, M.; Zhang, K. L.; Zeng, J.; Wang, L. J.; Xia, Z. N.; Gao, D. E. Anal. Chim. Acta 2019, 1084, 21.Gao, M. J.; Fu, Q. F.; Wang, M.; Zhang, K. L.; Zeng, J.; Wang, L. J.; Xia, Z. N.; Gao, D. E. Anal. Chim. Acta 2019, 1084, 21.
3. [3]
  Rocío-Bautista, P.; Pino, V.; Pasán, J.; López-Hernández, I.; Ayala, J. H.; Ruiz-Pérez, C.; Afonso A. M. Talanta 2018, 179, 775.Rocío-Bautista, P.; Pino, V.; Pasán, J.; López-Hernández, I.; Ayala, J. H.; Ruiz-Pérez, C.; Afonso A. M. Talanta 2018, 179, 775.
4. [4]
  Liu, L.; Wang, X. X.; Liu, F.; Xu, G. J.; Lin, J. M.; Wang, M. L.; Wu, Y. N.; Zhao, R. S.; Wang, X. Food Chem. 2022, 383, 132396.Liu, L.; Wang, X. X.; Liu, F.; Xu, G. J.; Lin, J. M.; Wang, M. L.; Wu, Y. N.; Zhao, R. S.; Wang, X. Food Chem. 2022, 383, 132396.
5. [5]
  Diana, M.; Felipe-Sotelo, M.; Bond, T. Water Res. 2019, 162, 492.Diana, M.; Felipe-Sotelo, M.; Bond, T. Water Res. 2019, 162, 492.
6. [6]
  Zhao, Y. L.; Qin, F; Boyd, J. M.; Anichina, J.; Li, X. F. Anal. Chem. 2010, 82, 4599.Zhao, Y. L.; Qin, F; Boyd, J. M.; Anichina, J.; Li, X. F. Anal. Chem. 2010, 82, 4599.
7. [7]
  曾尊祥, 胡玉玲, 胡玉斐, 肖华. 大学化学, 2024, 39 (3), 274.
8. [8]
  李攻科, 汪正范, 胡玉玲, 肖小华. 样品制备方法及应用. 第1版. 北京:化学工业出版社, 2023:209.
9. [9]
  台湾质谱学会. 质谱分析技术原理与应用. 第1版. 北京. 科学出版社, 2019:45.
10. [10]
  Xia, L.; Yang, J. N.; Su, R. H.; Zhou, W. J.; Zhang, Y. S.; Zhong, Y. H.; Huang, S. M.; Chen, Y. L.; Li, G. K. Anal. Chem. 2020, 92, 34.Xia, L.; Yang, J. N.; Su, R. H.; Zhou, W. J.; Zhang, Y. S.; Zhong, Y. H.; Huang, S. M.; Chen, Y. L.; Li, G. K. Anal. Chem. 2020, 92, 34.
11. [11]
  Ma, H. P.; Liu, B. L.; Li, B.; Zhang, L. M.; Li, Y. G.; Tan, H. Q.; Zang, H. Y.; Zhu, G. S. J. Am. Chem. Soc. 2016, 138, 5897.Ma, H. P.; Liu, B. L.; Li, B.; Zhang, L. M.; Li, Y. G.; Tan, H. Q.; Zang, H. Y.; Zhu, G. S. J. Am. Chem. Soc. 2016, 138, 5897.

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沈阳化工大学材料科学与工程学院沈阳 110142

分散固相萃取-液相色谱-三重四级杆质谱法分析水样中卤代醌类化合物

English

Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry

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中国化学会秘书处

分散固相萃取-液相色谱-三重四级杆质谱法分析水样中卤代醌类化合物

English

Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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