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Citation: GU Xinyu, LANG Le, WANG Jianwei, ZHAO Lihui. Determination of Aflatoxin B1 in Food by LC-MS/MS Method Based on Magnetic Bead-Aptamer[J]. Chinese Journal of Applied Chemistry, ;2020, 37(11): 1324-1332. doi: 10.11944/j.issn.1000-0518.2020.11.200125 shu

Determination of Aflatoxin B1 in Food by LC-MS/MS Method Based on Magnetic Bead-Aptamer

  • a.

    College of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China

  • b.

    Jilin Institute for Food Control, Changchun 130103, China

  • c.

    National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Figures(6)

  • In this study, a magnetic bead-aptamer that specifically recognizes aflatoxin B1 (AFB1) was constructed and coupled with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) to establish a quantitative detection method for AFB1 in food. Carboxy magnetic beads were activated by carbodiimide hydrochloride (EDC) and incubated with 5'terminal amino modified aptamers. The aptamers were covalently linked to the surface of the carboxy magnetic beads by amidation reaction and used as capture probes to separate AFB1 in the extracted sample. The qualitative and quantitative analysis of AFB1 was carried out by LC-MS/MS. The results show that the method has a good linear relationship at the concentration of 0.25~25 ng/mL with the correlation coefficient R2=0.999, the quantitative detection limit is 0.25 ng/mL, the recovery is 80.3%~92.5%, and the relative standard deviation (RSD) is less than 8%. The method is simple, fast and convenient, and can detect trace AFB1. The magnetic bead aptamer can be reused, which provides another technical support for the quantitative detection of AFB1.
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