Citation: ZHANG Yuhui, XING Ligang, CHENG Jiawei, YANG Jidong. Chiral Analysis of Propranolol and Its Aptamer System by Resonance Rayleigh Scattering Spectral Method[J]. Chinese Journal of Applied Chemistry, ;2020, 37(3): 359-366. doi: 10.11944/j.issn.1000-0518.2020.03.190252 shu

Chiral Analysis of Propranolol and Its Aptamer System by Resonance Rayleigh Scattering Spectral Method

a.
School of Environmental and Chemical Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing 404000, China
b.
School of Life Science and Technology, Yangtze Normal University; Fuling, Chongqing 408000, China

Corresponding author: YANG Jidong, sxyjd7600@sina.com
Received Date: 23 September 2019
Revised Date: 4 December 2019
Accepted Date: 16 January 2020

Fund Project: Supported by the National Natural Science Foundation of China(No.21175015, No.21475014)the National Natural Science Foundation of China 21475014the National Natural Science Foundation of China 21175015

Figures(7)

This paper presents a new method for chiral identification and analysis of propranolol. The method quoted the systematic evolution of ligands by exponential enrichment technique based on graphene oxide (GO-SELEX). The specific aptamer with high affinity to the cardiovascular drug propranolol was selected after ten rounds of optimization and screening. Then resonance Rayleigh scattering spectroscopy (RRS) was used to detect the specificity of the reaction system. The experiment results showed that S-propranolol and R-propranolol had utterly spectral differences. The RRS spectrum of S-propranolol combined with the specific aptamer was significantly enhanced, while the RRS spectrum of R-propranolol's was almost unchanged. Therefore, the chiral propranolol could be effectively recognized. Based on the investigation of the reaction system and experimental conditions, S-propranolol could be tested experimentally and R-propranolol in the racemate can be calculated. The linear range of S-propranolol was 5~275 nmol/L and the detection limit was 0.5 nmol/L. The method was applied to the determination of racemic tablets with satisfactory results. The RRS spectra of chiral targets system binded with specific aptamer could reveal chirality differences and the chiral recognition of chiral enantiomers can be carried out.
- S-/R-propranolol,
- aptamer,
- resonance Rayleigh scattering,
- chiral analysis
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沈阳化工大学材料科学与工程学院沈阳 110142