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Citation: HE Yunshan, LIU Yawei, TAN Zhoujin, HUI Huaying. Comparison of Two Lactase Activity Determination Methods[J]. Chinese Journal of Applied Chemistry, ;2020, 37(2): 242-244. doi: 10.11944/j.issn.1000-0518.2020.02.190215 shu

Comparison of Two Lactase Activity Determination Methods

a.
College of Pharmacy, Changsha 410208, China
b.
School of Medicine, Hunan University of Chinese Medicine, Changsha 410208, China

Corresponding author: HUI Huaying, 121142730@qq.com
Received Date: 3 August 2019
Revised Date: 4 September 2019
Accepted Date: 19 November 2019

Fund Project: the National Natural Science Foundation of China 81703985Supported by the National Natural Science Foundation of China(No.81703985)

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Both o-nitrophenyl β-D-galactopyranoside(ONPG) method and disaccharase method are commonly used for the determination of lactase activity. This paper compared the linear range, detection limit, precision, accuracy and stability of these two methods. It is found that ONPG method is better than disaccharase method in linear range, detection limit, precision and stability, but this method has poor repeatability and needs further optimization.
- o-nitrophenyl-β-D-galactopyranoside,
- disaccharase method,
- lactase activity,
- methodological comparison
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