Citation: DONG Yuman, MENG Shixin, QU Fengmei, SUN Hejia, TANG Qiming, WANG Yaolei, MENG Tao. Microfluidic Droplets Flow for Urea Detection Based on Enzymatic Reaction in Aqueous Two-Phase System[J]. Chinese Journal of Applied Chemistry, ;2020, 37(2): 211-217. doi: 10.11944/j.issn.1000-0518.2020.02.190214 shu

Microfluidic Droplets Flow for Urea Detection Based on Enzymatic Reaction in Aqueous Two-Phase System

School of Life Science and Engineering, South West Jiaotong University, Chengdu 610031, China

Corresponding author: MENG Tao, taomeng@swjtu.edu.cn
Received Date: 1 August 2019
Revised Date: 11 October 2019
Accepted Date: 14 November 2019

Fund Project: Supported by the National Natural Science Foundation of China(No.21776230)the National Natural Science Foundation of China 21776230

Figures(6)

A simple and rapid microfluidic enzymatic reaction method was used to visualize the urea concentration. In the aqueous two-phase system (ATPS), ammonium carbonate produced by the enzymatic reaction discolors the neutral red indicator in droplets, and the color intensity of droplets is analyzed to determine the concentration of urea in the sample to be tested. The detection range can reach 0~50 mg/mL. The microfluidic ATPS droplet flow overcomes the challenge of low biocompatibility of traditional detection platforms based on oil-water droplet. Less consumption, extremely large specific surface area and micro-scale diffusion distance of droplet flow result in the high enzymatic reaction rate. The reaction rate is many times faster than that of traditional beaker method. The detection time is reduced to 20 s. The study aims to provide new ideas and reference for the rapid urea detection in the field of applied chemistry.
- urea detection,
- microfluidics,
- aqueous two-phase system,
- droplet flow,
- enzymatic reaction
1. [1]
  Llopis Lorente A, Villalonga R, Marcos M D. A Versatile New Paradigm for the Design of Optical Nanosensors Based on Enzyme-Mediated Detachment of Labeled Reporters:The Example of Urea Detection[J]. Chem Eur J, 2019,25(14):3575-3581. doi: 10.1002/chem.201804706
2. [2]
  Trivedi U B, Lakshminarayana D, Kothari I L. Potentiometric Biosensor for Urea Determination in Milk[J]. Sens Actuators B, 2009,140(1):260-266.
3. [3]
  WEI Yongfeng, MA Dongmei, LI Xiaohua. Chitosan Immobillized Urease and Analysis of Urea in Waste Water[J]. Chinese J Appl Chem, 2004,21(4):357-360. doi: 10.3969/j.issn.1000-0518.2004.04.007
4. [4]
  YANG Yadong. Comparison of Two Methods for Determination of Urea in Water-P-dimethylaminobenzaldehyde Colorimetric Method and Kjeldahl Method[J]. Chem Enterp Manage, 2015,34:201-201.
5. [5]
  WANG Tiejun, SHI Hanqin, HUI Jianming, et al. A Fast Method for Detecting the Content of Adulterated Urea in Milk: CN, 105466906A[P]. 2015-12-16(in Chinese).
6. [6]
  HU Shenghua, LU Yunping, CHU Qingcui. Determination of Urea in Human Saliva by Capillary Electrophoresis with Electrochemical Detection[J]. J Instrum Anal, 2008,27(4):440-442. doi: 10.3969/j.issn.1004-4957.2008.04.025
7. [7]
  GAO Shizhong, LV Jiefang, LV Yongchang. Rapid Qualitative Analysis of Urea and Bovine Urine in Milk[J]. China Dariy Ind, 1986,14(3):76-78.
8. [8]
  YANG Shuiyun, MA Xiaoming, XU Fan, et al. Preparation of Urea Test Paper in Dairy Product and Urea Detection Method: CN, 101398386[P]. 2009-04-01(in Chinese).
9. [9]
  YU Dongwei, ZHAO Yuan, LIU Zhinan, et al. Method for Qualitative Detection of Urea in Milk: CN, 102539421A[P]. 2012-07-04(in Chinese).
10. [10]
  Mogensen K B, Klank H, Kutter J P. Recent Developments in Detection for Microfluidic Systems[J]. Electrophoresis, 2004,25(21/22):3498-3512.
11. [11]
  Whitesides G M. The Origins and the Future of Microfluidics[J]. Nature, 2006,442(7101):368-73. doi: 10.1038/nature05058
12. [12]
  ZHANG Maojie, WANG Wei, JU Xiaojie. Research Progress on Controllable Fabrication of Functional Microfibers from Microfluidic Jet Templates[J]. Chinese J Appl Chem, 2017,34(11):1240-1249. doi: 10.11944/j.issn.1000-0518.2017.11.170264
13. [13]
  Piao Y, Han D J, Azad M R. Enzyme Incorporated Microfluidic Device for in-situ Glucose Detection in Water-in-Air Microdroplets[J]. Biosens Bioelectron, 2015,65:220-225. doi: 10.1016/j.bios.2014.10.032
14. [14]
  Wang J, Chatrathi M, Ibañez A. Glucose Biochip:Dual Analyte Response in Connection to Two Pre-column Enzymatic Reactions[J]. Analyst, 2001,126(8):1203-1206. doi: 10.1039/b103193g
15. [15]
  Juul S, Nielsen C J F, Labouriau R. Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-Causing Plasmodium Parasites Based on Enzyme Activity Measurement[J]. ACS Nano, 2012,6(12):10676-10683. doi: 10.1021/nn3038594
16. [16]
  Agresti J J, Antipov E, Abate A R. Ultrahigh-Throughput Screening in Drop-Based Microfluidics for Directed Evolution[J]. Proc Natl Acad Sci USA, 2010,107(9):4004-4009. doi: 10.1073/pnas.0910781107
17. [17]
  Liu H T, Wang H, Wei W B. A Microfluidic Strategy for Controllable Generation of Water-In-Water Droplets as Biocompatible Microcarriers[J]. Small, 2018,14(36):1801095-1801099. doi: 10.1002/smll.201801095
18. [18]
  Meng S X, Xue L H, Xie C Y. Enhanced Enzymatic Reaction by Aqueous Two-Phase Systems Using Parallel-Laminar Flow in a Double Y-Branched Microfluidic Device[J]. Chem Eng J, 2018,335:392-400. doi: 10.1016/j.cej.2017.10.085
19. [19]
  Moon B, Jones S G, Hwang D K. Microfluidic Generation of Aqueous Two-Phase System(ATPS) Droplets by Controlled Pulsating Inlet Pressures[J]. Lab Chip, 2015,15(11):2437-2444. doi: 10.1039/C5LC00217F
20. [20]
  Mak S Y, Chao Y, Shum H C. The Dripping-to-Jetting Transition in a Co-axial Flow of Aqueous Two-Phase Systems with Low Interfacial Tension[J]. RSC Adv, 2017,7(6):3287-3292. doi: 10.1039/C6RA26556A
21. [21]
  Mastiani M, Seo S, Jimenez S. Flow Regime Mapping of Aqueous Two-Phase System Droplets in Flow-Focusing Geometries[J]. Colloids Surf A, 2017,531:111-120. doi: 10.1016/j.colsurfa.2017.07.083
22. [22]
  HJ 634, Soil-Determination of Ammonium, Nitrite and Nitrate by Extraction with Potassium Chloride Solution-Spectrophotometric Methods[S]. China Environmental Science Press, 2012(in Chinese).
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