Citation: LI Lin, SONG Jixiang, LI Yingqi, ZHANG Caifeng. Preparation of Lysozyme-Responsive Nanodiamond Drug-Loading System and the Effect on HepG2 Cells[J]. Chinese Journal of Applied Chemistry, ;2019, 36(10): 1109-1117. doi: 10.11944/j.issn.1000-0518.2019.10.190112 shu

Preparation of Lysozyme-Responsive Nanodiamond Drug-Loading System and the Effect on HepG2 Cells

LI Lin ^a,, ,
SONG Jixiang ^a ,
LI Yingqi ^c ,
ZHANG Caifeng ^a,b

a.
Department of Chemistry, Taiyuan Normal University, Jinzhong, Shanxi 030619, China
b.
Humic Acid Engineering and Technology Research Center of Shanxi Province, Jinzhong, Shanxi 030619, China
c.
Department of Chemistry, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China

Corresponding author: LI Lin, lilin@tynu.edu.cn
Received Date: 16 April 2019
Revised Date: 17 June 2019
Accepted Date: 17 August 2019

Fund Project: Supported by Shanxi Youth Science and Technology Research Fund Project(No.201801D221440), the Taiyuan Normal University College Students Science and Technology Innovation Project(No.2018503 CXCY1850), and the Shanxi Provincial Applied Fundamental Research Fund Project(No.201801D121257)Shanxi Youth Science and Technology Research Fund Project 201801D221440the Taiyuan Normal University College Students Science and Technology Innovation Project 2018503 CXCY1850the Shanxi Provincial Applied Fundamental Research Fund Project 201801D121257

Figures(11)

Glycidol(GLY) and doxorubicin(DOX) were coupled to ACAylated nanodiamond(ND) carrier to obtain nanodiamond-aminocaproic acid-glycidol-doxorubicin (ND-ACA-GLY-DOX, NAGD) drug delivery system through ester bond. The amounts of ACA and DOX coupled to the surface of the ND were (185±10.0) μg/mg and (115±5.2) μg/mg, respectively, as determined by fluorescence spectroscopy. The results showed that the amount of NAGD released was very low in the physiological environment(pH=7.4), while the ester bond was hydrolytically cleaved and a large amount of DOX was released in the presence of lysozyme in the lysosome environment of the tumor cells. Using liver cancer cells as the model, the cell morphology test showed that NAGD could effectively kill tumor cells. The above results indicate that NAGD can be used as a good drug delivery system.
- nanodiamond,
- aminocaproic acid,
- glycidol,
- doxorubicin,
- lysozyme responsive
1. [1]
  GolobSchwarzl N, Krassnig S, Toeglhofer A M. New Liver Cancer Biomarkers:PI3K/AKT/mTOR Pathway Members and Eukaryotic Translation Initiation Factors[J]. Eur J Cancer, 2017,83:56-70. doi: 10.1016/j.ejca.2017.06.003
2. [2]
  Zhang H L, Song Y, Yang H M. Tumor Cell-intrinsic Tim-3 Promotes Lver Cancer via NF-κB/IL-6/STAT3 Axis[J]. Oncogene, 2018,37:2456-2468. doi: 10.1038/s41388-018-0140-4
3. [3]
  Maucort-Boulch D, de Martel C, Franceschi S, et al. Fraction and Incidence of Liver Cancer Attributable to Hepatitis B and C Viruses Worldwide[J]. Int J Cancer, 142: 2471-2477.
4. [4]
  Yin H S, Que R S, Liu C Y. Survivin-Targeted Drug Screening Platform Identifies a Matrine Derivative WM-127 as a Potential Therapeutics Against Hepato Cellular Carcinoma[J]. Cancer Lett, 2018,425:54-64. doi: 10.1016/j.canlet.2018.03.044
5. [5]
  Fraser S, Antonella V, Qiu Y Q. A Prototype Therapeutic Capsule Endoscope for Ultrasound-Mediated Targeted Drug Delivery[J]. J Med Robot Res, 2018,3:1-13. doi: 10.5603/MRJ.2018.0001
6. [6]
  Wang Y R, Yang S Y, Chen G X. Barbaloin Loaded Polydopamine-Polylactide-TPGS(PLA-TPGS) Nanoparticles Against Gastric Cancer as a Targeted Drug Delivery System:Studies in Vitro and in Vivo[J]. Biochem Biophys Res Commun, 2018,499(1):8-16. doi: 10.1016/j.bbrc.2018.03.069
7. [7]
  Umberto C, Hsia F C, Danielle V. Mesoscopic Physical Removal of Material Using Sliding Nanodiamond Contacts[J]. Sci Rep, 2018,8:2994-3005. doi: 10.1038/s41598-018-21171-w
8. [8]
  Larsson K, Tian Y. Effect of Surface Termination on the Reactivity of Nano-sized Diamond Particle Surfaces for Bio Applications[J]. Carbon, 2018,134:244-254. doi: 10.1016/j.carbon.2018.03.031
9. [9]
  Xi G F, Robinson E, Mania-Farnell B. Convection-Enhanced Delivery of Nanodiamond Drug Delivery Platforms for Intracranial Tumor Treatment[J]. Nanomed-Nanotechnol Biol Med, 2014,10:381-391. doi: 10.1016/j.nano.2013.07.013
10. [10]
  Chow E K, Zhang X Q, Chen M. Nanodiamond Therapeutic Delivery Agents Mediate Enhanced Chemoresistant Tumor Treatment[J]. Sci Transl Med, 2011,373 ra 21.
11. [11]
  Wang D X, Tong Y L, Li Y Q. PEGylated Nanodiamond for Chemotherapeutic Drug Delivery[J]. Diamond Relat Mater, 2013,36:26-34. doi: 10.1016/j.diamond.2013.04.002
12. [12]
  Dong Y, Cao R X, Li Y Q. Folate-Conjugated Nanodiamond for Tumor-Targeted Drug Delivery[J]. RSC Adv, 2015,5:82711-82716. doi: 10.1039/C5RA12383F
13. [13]
  CAO Ruixia, TIAN Zhimei, WANG Dongxin. Preparation of Folic Acid Functionalized Nanodiamonds Loading Drug and Its Targeted Research of C6 Cells[J]. J Shanxi Univ, 2014,37(1):91-97.
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