Citation: WANG Xueliang, WANG Zhaoxia, WANG Tao, DAI Xiaohui. Electrochemical Determination of the Specific Genetic Sequence in Transgenic Maize Using Reduced Graphene Oxide and Nano Zirconia Composites as a Platform for Immobilizing DNA[J]. Chinese Journal of Applied Chemistry, ;2019, 36(7): 839-846. doi: 10.11944/j.issn.1000-0518.2019.07.180378 shu

Electrochemical Determination of the Specific Genetic Sequence in Transgenic Maize Using Reduced Graphene Oxide and Nano Zirconia Composites as a Platform for Immobilizing DNA

a.
College of Chemistry and Chemical Engineering, Heze University, Heze, Shandong 274015, China
b.
College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China

Corresponding author: WANG Xueliang, qust-1977wxl@163.com
Received Date: 28 November 2018
Revised Date: 26 February 2019
Accepted Date: 10 April 2019

Fund Project: the Natural Science Foundation Committee of Shandong Province, China ZR2015BL014the Natural Science Foundation Committee of Shandong Province, China ZR2017MB062Supported by the National Natural Science Foundation of China(No.21105023), the Natural Science Foundation Committee of Shandong Province, China(No.ZR2017MB062, No.ZR2015BL014)the National Natural Science Foundation of China 21105023

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The phosphinothricin acetyltransferase(PAT) gene is a kind of exogenous DNA segments indicating genetically modified plants. In this research, a sensitive method for determination of the PAT gene in transgenic maize has been developed using reduced graphene oxide and nano zirconia(nanoZrO₂) composites as a platform for immobilizing DNA probes. First, the graphene oxide was direct electrochemically reduced on a glassy carbon electrode(GCE); then, a layer of nanoZrO₂ was covered on the modified electrode. Last, the DNA probes were immobilized via the affinity between phosphate groups in the DNA probes and the oxygen in nanoZrO₂. The hybridization of the DNA probes with the PAT gene segments was monitored by differential pulse voltammetry(DPV). The sensor was stable, reproducible and sensitive to effectively discriminate the PAT gene in transgenic maize. The detection limit was 2.0×10^-15 mol/L.
- differential pulse voltammetry,
- reduced graphene oxide,
- nano zirconia,
- phosphinothricin acetyltransferase gene,
- transgenic maize
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沈阳化工大学材料科学与工程学院沈阳 110142