Citation: CHENG Xiaodong, ZHANG Zheng. Preparation and Chromatographic Evaluation of a L-Isoleucine-Bonded Chromatographic Stationary Phase[J]. Chinese Journal of Applied Chemistry, ;2019, 36(6): 726-732. doi: 10.11944/j.issn.1000-0518.2019.06.180356 shu

Preparation and Chromatographic Evaluation of a L-Isoleucine-Bonded Chromatographic Stationary Phase

CHENG Xiaodong ^a,, ,
ZHANG Zheng ^b

a.
Qiannan Normal College For Nationalities, Duyun, Guizhou 558000, China
b.
Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China

Corresponding author: CHENG Xiaodong, shapaozi1995@163.com
Received Date: 8 November 2018
Revised Date: 11 January 2019
Accepted Date: 4 March 2019

Fund Project: the Project of the Science and Technology Department in Guizhou Province LH[2015]7714the Project of the Science and Technology Department in Guizhou Province Qian ke he ji chu [2017]1169the Project for the Growth of Young Science and Technology Talents in Guizhou Province Qian jiao he KY[2018]422Supported by the Project of the Science and Technology Department in Guizhou Province(No.Qian ke he ji chu [2017]1169), the Project for the Growth of Young Science and Technology Talents in Guizhou Province(No.Qian jiao he KY[2018]422), the Project of the Science and Technology Department in Guizhou Province(No.LH[2015]7714)

Figures(6)

L-Isoleucine-bonded silica stationary phase was prepared starting from L-isoleucine and 3-isocyanatopropyltriethoxysilane, and further reaction between the resulting L-isoleucine functionalized silane coupling agents and activated silica. The result of ¹H NMR spectroscopy showed that the expected L-isoleucine functionalized silane was successfully synthesized. The successful grafting was further confirmed by elemental analysis. The synthesized stationary phase was slurry-packed into stainless steel columns(150 mm×4.6 mm). A set of typical polar compounds were employed to evaluate the chromatographic behaviors on the stationary phase in hydrophilic interaction liguid chromatography(HILIC) mode. Increased retention factors were observed for these compounds with the increase of acetonitrile(ACN) content in the mobile phase, demonstrating a typical HILIC retention characteristics. Additionally, the effect of various parameters, such as ACN content, pH values and ionic strength of the mobile phase on the retention of the tested solutes were investigated. Under optimized conditions, 5 basic compounds, 6 water-soluble vitamins and 8 nucleosides were successfully separated within 8 min, 18 min and 25 min, respectively, by isocratic elution. The resulting separation chromatogram exhibits remarkable chromatographic performance, demonstrating the excellent application potential of the L-isoleucine-bonded silica stationary phase in the separation of polar compounds.
- L-isoleucine,
- stationary phase,
- hydrophilic interaction liguid chromatography,
- retention mechanism
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