Citation: WEI Guobing, KONG Derong, XU Huihui, YIN Zhaojiang, ZHANG Jing, CUI Hanfeng, HONG Nian, YANG Jie, XIONG Wei, LIU Wenming, GUO Qianwen, CHENG Lin, FAN Hao. A New Type of Electrochemiluminescence Sensor for Detection of Mercury Ion in Chinese Medicinal Materials Danshen Based on Host-Guest Interaction[J]. Chinese Journal of Applied Chemistry, ;2019, 36(5): 595-602. doi: 10.11944/j.issn.1000-0518.2019.05.180310 shu

A New Type of Electrochemiluminescence Sensor for Detection of Mercury Ion in Chinese Medicinal Materials Danshen Based on Host-Guest Interaction

Department of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China

Corresponding author: CHENG Lin, lincheng_2003@126.com FAN Hao, fanhao11@aliyun.com
Received Date: 20 September 2018
Revised Date: 11 December 2018
Accepted Date: 23 January 2019

Fund Project: Jiangxi Youth Natural Science Foundation 20161BAB215212Supported by the National Natural Science Foundation of China(No.81660658, No.81860682, No.81560625, No.81872968), Jiangxi Youth Natural Science Foundation(No.20161BAB215212), Traditional Chinese Medicine Program of Jiangxi Provincial Health Planning Commission(No.2017A280), Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine(No.JZYC17S07, No.JZYC18S13), Key Subject Support Program of Jiangxi University of Traditional Chinese Medicine(No.2017jzzdxk036), Science and Technology Project of Jiangxi Education Department(No.GJJ160833)Science and Technology Project of Jiangxi Education Department GJJ160833Key Subject Support Program of Jiangxi University of Traditional Chinese Medicine 2017jzzdxk036the National Natural Science Foundation of China 81660658the National Natural Science Foundation of China 81560625the National Natural Science Foundation of China 81872968Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine JZYC18S13Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine JZYC17S07Traditional Chinese Medicine Program of Jiangxi Provincial Health Planning Commission 2017A280the National Natural Science Foundation of China 81860682

Figures(7)

A new type of "Off-On" electrochemiluminescence(ECL) biosensor using Ru(bpy)₃²⁺/β-CD-AuNPs/Nafion complex and ferrocene-labeled DNA(Fc-DNA) was designed to detect mercury ions in Chinese medicinal materials Danshen. The ECL biosensor includes an ECL substrate and an ECL signal switch. Ru(bpy)₃²⁺/β-CD-AuNPs/Nafion complex modified glassy carbon electrode was used as the ECL substrate, which could bring about a clear and stable ECL signal by Ru(bpy)₃²⁺, and hairpin-like Fc-DNA probe acted as the ECL signal switch, which was designed by molecular recognition strategy and attached to β-CD-AuNPs through host-guest interaction between β-CD and ferrocene. Compared with previous methods of mercury ion detection, this method has the advantages of convenient operation, high sensitivity, good repeatability and low detection limit. The ECL biosensor offered good linear responses for Hg²⁺ in the range of 0.04~800 ng/mL with a detection limit of 0.02 ng/mL(S/N=3).
- electrochemiluminescence,
- biosensor,
- mercury(Ⅱ),
- molecular recognition technology,
- Switch
1. [1]
  FANG Wentao, DENG Aiping, REN Zhenli. Research Progress on Quality Evaluation of Salviae Miltiorrhizae Radix et Rhizoma(Danshen)[J]. China J Chinese Mater Med, 2018,43(6):1077-1085.
2. [2]
  SUN Yangzhao, CHEN Yang, LAN Hong. Source, Origin and Control Technology Path Analysis of Mercury Pollution in China[J]. Environ Chem, 2013,32(6):937-942.
3. [3]
  HE Chang. Analysis on Excessive Heavy Metal Problem of Chinese Medica Exports to EU from Perspective of UK Ban[J]. Chinese Tradit Herbal Drugs, 2016,47(10):1820-1824. doi: 10.7501/j.issn.0253-2670.2016.10.031
4. [4]
  LUO Hongli, HU Yichen, LUO Jiaoyang. Research Advances on Detecting Heavy Metals and Harmful Elements Based on Fluorescent Probe Quantum Dots[J]. Chinese Tradit Herbal Drugs, 2015,46(18):2809-2815. doi: 10.7501/j.issn.0253-2670.2015.18.024
5. [5]
  LI Fengwu, BAI Xue, LIU Shuo. Determination of Various Heavy Metal Elements in Radix Salviae Miltiorrhizae by ICP-AES Method[J]. Shaanxi J Tradit Chinese Med, 2016,37(11):1545-1546. doi: 10.3969/j.issn.1000-7369.2016.11.049
6. [6]
  XUE Yanli, XUE Xiaojian. Five Different Origin of Salvia Miltiorrhiza on the Determination of Heavy Metal Content in Medicine[J]. China Pharmacist, 2009,12(9):1321-1322. doi: 10.3969/j.issn.1008-049X.2009.09.080
7. [7]
  LI Xiuping, LI Haiyan, XIE Jianming. Determination of Two Kinds of Heavy Metals of Pb and Cd in Salvia Miltiorrhiza by Atomic Absorption Spectrometry[J]. Chinese Med Pharm, 2016,6(9):50-52.
8. [8]
  LIN Mingyue, LIANG Xiuqing. Determination of Arsenic and Mercury Content in Salvia Miltiorrhizaby Microwave Digestion-Atomic Fluorescence Spectrometry[J]. J Chinese Med Mat, 2016,39(5):1108-1110.
9. [9]
  Liu Y M, Zhang F P, Jiao B Y. Automated Dispersive Liquid-Liquid Microextraction Coupled to High Performance Liquid Chromatography-Cold Vapour Atomic Fluorescence Spectroscopy for the Determination of Mercury Species in Natural Water Samples[J]. J Chromatogr A, 2017,1493(1):1-9.
10. [10]
  Brent R N, Wines H, Luther J. Validation of Handheld X-Ray Fluorescence for in Situ Measurement of Mercury in Soils[J]. J Environ Chem Eng, 2017,5(1):768-776. doi: 10.1016/j.jece.2016.12.056
11. [11]
  Han A, Yang Y Y, Zhang Q. Electrochemistry and Electrochemiluminescence of Copper Metal Cluster[J]. J Electroanal Chem, 2017,795(1):116-122.
12. [12]
  Ono A, Togashi H. Highly Selective Oligonucleotide-Based Sensor for Mercury(Ⅱ) in Aqueous Solutions[J]. Angew Chem, 2004,43(33):4300-4302. doi: 10.1002/(ISSN)1521-3773
13. [13]
  Florian S, Bernhard V, Artur H. Redox-Switchable Supramolecular Graft Polymer Formation via Ferrocene Cyclodextrin Assembly[J]. Macromol Rapid Comm, 2014,35(14):1293-1300. doi: 10.1002/marc.v35.14
14. [14]
  Li Y, Qi H, Peng Y. Electrogenerated Chemiluminescence Aptamer-Based Method for the Determination of Thrombin in Corporating Quenching of Tris(2, 2'-bipyridine) Ruthenium by Ferrocene[J]. Electrochem Commun, 2008,10(9):1322-1325. doi: 10.1016/j.elecom.2008.06.027
15. [15]
  Wang X Y, Dong P, Yun W. Detection of T4 DNA Ligase Using a Solid-State Electrochemiluminescence Biosensing Switch Based on Ferrocene-Labeled Molecular Beacon[J]. Talanta, 2010,80(5):1643-1647. doi: 10.1016/j.talanta.2009.09.060
16. [16]
  Gadelle A, Defaye J. Selective Halogenation at Primary Positions of Cyclomaltooligosaccharides and a Synthesis of Per-3, 6-anhydro Cyclomaltooligosaccharides[J]. Angew Chem Int Ed, 1991,30(1):78-80.
17. [17]
  Liu J, Ong W, Roman E. Cyclodextrin-Modified Gold Nanospheres[J]. Langmuir, 2000,16(7):3000-3002. doi: 10.1021/la991519f
18. [18]
  Al-Hinaai M M, Kyaw H H, Al-Harthi S H. An Innovative ECL Sensor Based on AuNPs Linker for Ru(bpy)₃²⁺ Molecules Doped onto an Alkaline Pretreated Poly(4-aminodiphenylamine) Film[J]. Sens Actuators B, 2018,257(1):460-468.
19. [19]
  Huang J, Gao X, Jia J. Graphene Oxide-Based Amplified Fluorescent Biosensor for Hg²⁺ Detection Through Hybridization Chain Reactions[J]. Anal Chem, 2014,86(6):3209-3215. doi: 10.1021/ac500192r
20. [20]
  Wei H, Wang Z, Yang L. Lysozyme-Stabilized Gold Fluorescent Cluster:Synthesis and Application as Hg²⁺ Sensor[J]. Analyst, 2010,135(6):1406-1410. doi: 10.1039/c0an00046a
21. [21]
  Stobiecka M, Molinero A A, Chałupa A. Mercury/Homocysteine Ligation-Induced ON/OFF-Switching of a T-T Mismatch-Based Oligonucleotide Molecular Beacon[J]. Anal Chem, 2012,84(11)4970. doi: 10.1021/ac300632u
22. [22]
  Zhu Z, Su Y, Li J. Highly Sensitive Electrochemical Sensor for Mercury(Ⅱ) Ions by Using a Mercury-Specific Oligonucleotide Probe and Gold Nanoparticle-Based Amplification[J]. Anal Chem, 2009,81(18):7660-7666. doi: 10.1021/ac9010809
23. [23]
  Mor-Piperberg G, Tel-Vered R, Elbaz J. Nanoengineered Electrically Contacted Enzymes on DNA Scaffolds:Functional Assemblies for the Selective Analysis of Hg(Ⅱ) Ions[J]. J Am Chem Soc, 2010,132(20):6878-6879. doi: 10.1021/ja1006355
24. [24]
  Park J W, Park S J, Kwon O S. High-Performance Hg²⁺ FET-type Sensors Based on Reduced Graphene Oxide-Polyfuran Nanohybrids[J]. Analyst, 2014,139(16):3852-3855. doi: 10.1039/C4AN00403E
25. [25]
  Li D, Wieckowska A, Willner I. Optical Analysis of Hg(Ⅱ) Ions by Oligonucleotide-Gold-Nanoparticle Hybrids and DNA-Based Machines[J]. Angew Chemie, 2008,47(21):3927-3931. doi: 10.1002/anie.v47:21
26. [26]
  Huy G D, Zhang M, Zuo P. Multiplexed Analysis of Silver(Ⅰ) and Mercury(Ⅱ) Ions Using Oligonucletide-Metal Nanoparticle Conjugates[J]. Analyst, 2011,136(16):3289-3294.
27. [27]
  Yang Y K, Yook K J, Tae J. A Rhodamine-Based Fluorescent and Colorimetric Chemodosimeter for the Rapid Detection of Hg(Ⅱ) Ions in Aqueous Media[J]. J Am Chem Soc, 2005,127(48):16760-16761.
28. [28]
  Jiang Z, Fan Y, Chen M. Resonance Scattering Spectral Detection of Trace Hg(Ⅱ) Using Aptamer-Modified Nanogold as Probe and Nanocatalyst[J]. Anal Chem, 2009,81(13):5439-5445. doi: 10.1021/ac900590g
29. [29]
  Ren W, Zhang Y, Chen H G. Ultrasensitive Label-Free Resonance Rayleigh Scattering Aptasensor for Hg²⁺ Using Hg²⁺-Triggered Exonuclease Ⅲ-Assisted Target Recycling and Growth of G-Wires for Signal Amplication[J]. Anal Chem, 2016,88(2):1385-1390. doi: 10.1021/acs.analchem.5b03972
1. [1]
  Meiqing Yang , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-. doi: 10.3866/PKU.WHXB202310046
2. [2]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
3. [3]
  Rui Gao , Ying Zhou , Yifan Hu , Siyuan Chen , Shouhong Xu , Qianfu Luo , Wenqing Zhang . Design, Synthesis and Performance Experiment of Novel Photoswitchable Hybrid Tetraarylethenes. University Chemistry, 2024, 39(5): 125-133. doi: 10.3866/PKU.DXHX202310050
4. [4]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
5. [5]
  Minna Ma , Yujin Ouyang , Yuan Wu , Mingwei Yuan , Lijuan Yang . Green Synthesis of Medical Chemiluminescence Reagents by Photocatalytic Oxidation. University Chemistry, 2024, 39(5): 134-143. doi: 10.3866/PKU.DXHX202310093
6. [6]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . 倍增型全聚合物光电探测器及其在光电容积描记传感器上的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2311021-. doi: 10.3866/PKU.WHXB202311021
7. [7]
  Borong Yu , Huijiao Zhang , Xinyu Zhang , Xiaoying Li , Shuming Chen , Zhangang Han . The Blue Elf in the Dark: Gradient Science Popularization Experiments on Chemiluminescence. University Chemistry, 2024, 39(9): 295-303. doi: 10.12461/PKU.DXHX202403107
8. [8]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
9. [9]
  Siyu Zhang , Kunhong Gu , Bing'an Lu , Junwei Han , Jiang Zhou . Hydrometallurgical Processes on Recycling of Spent Lithium-lon Battery Cathode: Advances and Applications in Sustainable Technologies. Acta Physico-Chimica Sinica, 2024, 40(10): 2309028-. doi: 10.3866/PKU.WHXB202309028
10. [10]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
11. [11]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
12. [12]
  Shicheng Yan . Experimental Teaching Design for the Integration of Scientific Research and Teaching: A Case Study on Organic Electrooxidation. University Chemistry, 2024, 39(11): 350-358. doi: 10.12461/PKU.DXHX202408036
13. [13]
  Tianyun Chen , Ruilin Xiao , Xinsheng Gu , Yunyi Shao , Qiujun Lu . Synthesis, Crystal Structure, and Mechanoluminescence Properties of Lanthanide-Based Organometallic Complexes. University Chemistry, 2024, 39(5): 363-370. doi: 10.3866/PKU.DXHX202312017
14. [14]
  Shanghua Li , Malin Li , Xiwen Chi , Xin Yin , Zhaodi Luo , Jihong Yu . 基于高离子迁移动力学的取向ZnQ分子筛保护层实现高稳定水系锌金属负极的构筑. Acta Physico-Chimica Sinica, 2025, 41(1): 2309003-. doi: 10.3866/PKU.WHXB202309003
15. [15]
  Mingxin LU , Liyang ZHOU , Xiaoyu XU , Xiaoying FENG , Hui WANG , Bin YAN , Jie XU , Chao CHEN , Hui MEI , Feng GAO . Preparation of La-doped lead-based piezoelectric ceramics with both high electrical strain and Curie temperature. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 329-338. doi: 10.11862/CJIC.20240206
16. [16]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
17. [17]
  Wei Li , Guoqiang Feng , Ze Chang . Teaching Reform of X-ray Diffraction Using Synchrotron Radiation in Materials Chemistry. University Chemistry, 2024, 39(3): 29-35. doi: 10.3866/PKU.DXHX202308060
18. [18]
  Dongxue Han , Huiliang Sun , Li Niu . Virtual Reality Technology for Safe and Green University Chemistry Experimental Education. University Chemistry, 2024, 39(8): 191-196. doi: 10.3866/PKU.DXHX202312055
19. [19]
  Qingtang ZHANG , Xiaoyu WU , Zheng WANG , Xiaomei WANG . Performance of nano Li₂FeSiO₄/C cathode material co-doped by potassium and chlorine ions. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1689-1696. doi: 10.11862/CJIC.20240115
20. [20]
  Guang Huang , Lei Li , Dingyi Zhang , Xingze Wang , Yugai Huang , Wenhui Liang , Zhifen Guo , Wenmei Jiao . Cobalt’s Valor, Nickel’s Foe: A Comprehensive Chemical Experiment Utilizing a Cobalt-based Imidazolate Framework for Nickel Ion Removal. University Chemistry, 2024, 39(8): 174-183. doi: 10.3866/PKU.DXHX202311051

Get Citation

PDF

XML

Metrics

PDF Downloads(6)
Abstract views(474)
HTML views(52)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142