Citation: WEI Guobing, KONG Derong, XU Huihui, YIN Zhaojiang, ZHANG Jing, CUI Hanfeng, HONG Nian, YANG Jie, XIONG Wei, LIU Wenming, GUO Qianwen, CHENG Lin, FAN Hao. A New Type of Electrochemiluminescence Sensor for Detection of Mercury Ion in Chinese Medicinal Materials Danshen Based on Host-Guest Interaction[J]. Chinese Journal of Applied Chemistry, ;2019, 36(5): 595-602. doi: 10.11944/j.issn.1000-0518.2019.05.180310 shu

A New Type of Electrochemiluminescence Sensor for Detection of Mercury Ion in Chinese Medicinal Materials Danshen Based on Host-Guest Interaction

Department of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China

Corresponding author: CHENG Lin, lincheng_2003@126.com FAN Hao, fanhao11@aliyun.com
Received Date: 20 September 2018
Revised Date: 11 December 2018
Accepted Date: 23 January 2019

Fund Project: Jiangxi Youth Natural Science Foundation 20161BAB215212Supported by the National Natural Science Foundation of China(No.81660658, No.81860682, No.81560625, No.81872968), Jiangxi Youth Natural Science Foundation(No.20161BAB215212), Traditional Chinese Medicine Program of Jiangxi Provincial Health Planning Commission(No.2017A280), Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine(No.JZYC17S07, No.JZYC18S13), Key Subject Support Program of Jiangxi University of Traditional Chinese Medicine(No.2017jzzdxk036), Science and Technology Project of Jiangxi Education Department(No.GJJ160833)Science and Technology Project of Jiangxi Education Department GJJ160833Key Subject Support Program of Jiangxi University of Traditional Chinese Medicine 2017jzzdxk036the National Natural Science Foundation of China 81660658the National Natural Science Foundation of China 81560625the National Natural Science Foundation of China 81872968Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine JZYC18S13Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine JZYC17S07Traditional Chinese Medicine Program of Jiangxi Provincial Health Planning Commission 2017A280the National Natural Science Foundation of China 81860682

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A new type of "Off-On" electrochemiluminescence(ECL) biosensor using Ru(bpy)₃²⁺/β-CD-AuNPs/Nafion complex and ferrocene-labeled DNA(Fc-DNA) was designed to detect mercury ions in Chinese medicinal materials Danshen. The ECL biosensor includes an ECL substrate and an ECL signal switch. Ru(bpy)₃²⁺/β-CD-AuNPs/Nafion complex modified glassy carbon electrode was used as the ECL substrate, which could bring about a clear and stable ECL signal by Ru(bpy)₃²⁺, and hairpin-like Fc-DNA probe acted as the ECL signal switch, which was designed by molecular recognition strategy and attached to β-CD-AuNPs through host-guest interaction between β-CD and ferrocene. Compared with previous methods of mercury ion detection, this method has the advantages of convenient operation, high sensitivity, good repeatability and low detection limit. The ECL biosensor offered good linear responses for Hg²⁺ in the range of 0.04~800 ng/mL with a detection limit of 0.02 ng/mL(S/N=3).
- electrochemiluminescence,
- biosensor,
- mercury(Ⅱ),
- molecular recognition technology,
- Switch
1. [1]
  FANG Wentao, DENG Aiping, REN Zhenli. Research Progress on Quality Evaluation of Salviae Miltiorrhizae Radix et Rhizoma(Danshen)[J]. China J Chinese Mater Med, 2018,43(6):1077-1085.
2. [2]
  SUN Yangzhao, CHEN Yang, LAN Hong. Source, Origin and Control Technology Path Analysis of Mercury Pollution in China[J]. Environ Chem, 2013,32(6):937-942.
3. [3]
  HE Chang. Analysis on Excessive Heavy Metal Problem of Chinese Medica Exports to EU from Perspective of UK Ban[J]. Chinese Tradit Herbal Drugs, 2016,47(10):1820-1824. doi: 10.7501/j.issn.0253-2670.2016.10.031
4. [4]
  LUO Hongli, HU Yichen, LUO Jiaoyang. Research Advances on Detecting Heavy Metals and Harmful Elements Based on Fluorescent Probe Quantum Dots[J]. Chinese Tradit Herbal Drugs, 2015,46(18):2809-2815. doi: 10.7501/j.issn.0253-2670.2015.18.024
5. [5]
  LI Fengwu, BAI Xue, LIU Shuo. Determination of Various Heavy Metal Elements in Radix Salviae Miltiorrhizae by ICP-AES Method[J]. Shaanxi J Tradit Chinese Med, 2016,37(11):1545-1546. doi: 10.3969/j.issn.1000-7369.2016.11.049
6. [6]
  XUE Yanli, XUE Xiaojian. Five Different Origin of Salvia Miltiorrhiza on the Determination of Heavy Metal Content in Medicine[J]. China Pharmacist, 2009,12(9):1321-1322. doi: 10.3969/j.issn.1008-049X.2009.09.080
7. [7]
  LI Xiuping, LI Haiyan, XIE Jianming. Determination of Two Kinds of Heavy Metals of Pb and Cd in Salvia Miltiorrhiza by Atomic Absorption Spectrometry[J]. Chinese Med Pharm, 2016,6(9):50-52.
8. [8]
  LIN Mingyue, LIANG Xiuqing. Determination of Arsenic and Mercury Content in Salvia Miltiorrhizaby Microwave Digestion-Atomic Fluorescence Spectrometry[J]. J Chinese Med Mat, 2016,39(5):1108-1110.
9. [9]
  Liu Y M, Zhang F P, Jiao B Y. Automated Dispersive Liquid-Liquid Microextraction Coupled to High Performance Liquid Chromatography-Cold Vapour Atomic Fluorescence Spectroscopy for the Determination of Mercury Species in Natural Water Samples[J]. J Chromatogr A, 2017,1493(1):1-9.
10. [10]
  Brent R N, Wines H, Luther J. Validation of Handheld X-Ray Fluorescence for in Situ Measurement of Mercury in Soils[J]. J Environ Chem Eng, 2017,5(1):768-776. doi: 10.1016/j.jece.2016.12.056
11. [11]
  Han A, Yang Y Y, Zhang Q. Electrochemistry and Electrochemiluminescence of Copper Metal Cluster[J]. J Electroanal Chem, 2017,795(1):116-122.
12. [12]
  Ono A, Togashi H. Highly Selective Oligonucleotide-Based Sensor for Mercury(Ⅱ) in Aqueous Solutions[J]. Angew Chem, 2004,43(33):4300-4302. doi: 10.1002/(ISSN)1521-3773
13. [13]
  Florian S, Bernhard V, Artur H. Redox-Switchable Supramolecular Graft Polymer Formation via Ferrocene Cyclodextrin Assembly[J]. Macromol Rapid Comm, 2014,35(14):1293-1300. doi: 10.1002/marc.v35.14
14. [14]
  Li Y, Qi H, Peng Y. Electrogenerated Chemiluminescence Aptamer-Based Method for the Determination of Thrombin in Corporating Quenching of Tris(2, 2'-bipyridine) Ruthenium by Ferrocene[J]. Electrochem Commun, 2008,10(9):1322-1325. doi: 10.1016/j.elecom.2008.06.027
15. [15]
  Wang X Y, Dong P, Yun W. Detection of T4 DNA Ligase Using a Solid-State Electrochemiluminescence Biosensing Switch Based on Ferrocene-Labeled Molecular Beacon[J]. Talanta, 2010,80(5):1643-1647. doi: 10.1016/j.talanta.2009.09.060
16. [16]
  Gadelle A, Defaye J. Selective Halogenation at Primary Positions of Cyclomaltooligosaccharides and a Synthesis of Per-3, 6-anhydro Cyclomaltooligosaccharides[J]. Angew Chem Int Ed, 1991,30(1):78-80.
17. [17]
  Liu J, Ong W, Roman E. Cyclodextrin-Modified Gold Nanospheres[J]. Langmuir, 2000,16(7):3000-3002. doi: 10.1021/la991519f
18. [18]
  Al-Hinaai M M, Kyaw H H, Al-Harthi S H. An Innovative ECL Sensor Based on AuNPs Linker for Ru(bpy)₃²⁺ Molecules Doped onto an Alkaline Pretreated Poly(4-aminodiphenylamine) Film[J]. Sens Actuators B, 2018,257(1):460-468.
19. [19]
  Huang J, Gao X, Jia J. Graphene Oxide-Based Amplified Fluorescent Biosensor for Hg²⁺ Detection Through Hybridization Chain Reactions[J]. Anal Chem, 2014,86(6):3209-3215. doi: 10.1021/ac500192r
20. [20]
  Wei H, Wang Z, Yang L. Lysozyme-Stabilized Gold Fluorescent Cluster:Synthesis and Application as Hg²⁺ Sensor[J]. Analyst, 2010,135(6):1406-1410. doi: 10.1039/c0an00046a
21. [21]
  Stobiecka M, Molinero A A, Chałupa A. Mercury/Homocysteine Ligation-Induced ON/OFF-Switching of a T-T Mismatch-Based Oligonucleotide Molecular Beacon[J]. Anal Chem, 2012,84(11)4970. doi: 10.1021/ac300632u
22. [22]
  Zhu Z, Su Y, Li J. Highly Sensitive Electrochemical Sensor for Mercury(Ⅱ) Ions by Using a Mercury-Specific Oligonucleotide Probe and Gold Nanoparticle-Based Amplification[J]. Anal Chem, 2009,81(18):7660-7666. doi: 10.1021/ac9010809
23. [23]
  Mor-Piperberg G, Tel-Vered R, Elbaz J. Nanoengineered Electrically Contacted Enzymes on DNA Scaffolds:Functional Assemblies for the Selective Analysis of Hg(Ⅱ) Ions[J]. J Am Chem Soc, 2010,132(20):6878-6879. doi: 10.1021/ja1006355
24. [24]
  Park J W, Park S J, Kwon O S. High-Performance Hg²⁺ FET-type Sensors Based on Reduced Graphene Oxide-Polyfuran Nanohybrids[J]. Analyst, 2014,139(16):3852-3855. doi: 10.1039/C4AN00403E
25. [25]
  Li D, Wieckowska A, Willner I. Optical Analysis of Hg(Ⅱ) Ions by Oligonucleotide-Gold-Nanoparticle Hybrids and DNA-Based Machines[J]. Angew Chemie, 2008,47(21):3927-3931. doi: 10.1002/anie.v47:21
26. [26]
  Huy G D, Zhang M, Zuo P. Multiplexed Analysis of Silver(Ⅰ) and Mercury(Ⅱ) Ions Using Oligonucletide-Metal Nanoparticle Conjugates[J]. Analyst, 2011,136(16):3289-3294.
27. [27]
  Yang Y K, Yook K J, Tae J. A Rhodamine-Based Fluorescent and Colorimetric Chemodosimeter for the Rapid Detection of Hg(Ⅱ) Ions in Aqueous Media[J]. J Am Chem Soc, 2005,127(48):16760-16761.
28. [28]
  Jiang Z, Fan Y, Chen M. Resonance Scattering Spectral Detection of Trace Hg(Ⅱ) Using Aptamer-Modified Nanogold as Probe and Nanocatalyst[J]. Anal Chem, 2009,81(13):5439-5445. doi: 10.1021/ac900590g
29. [29]
  Ren W, Zhang Y, Chen H G. Ultrasensitive Label-Free Resonance Rayleigh Scattering Aptasensor for Hg²⁺ Using Hg²⁺-Triggered Exonuclease Ⅲ-Assisted Target Recycling and Growth of G-Wires for Signal Amplication[J]. Anal Chem, 2016,88(2):1385-1390. doi: 10.1021/acs.analchem.5b03972
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