Citation: HUANG Zhenlong, CHEN Lingcheng, XIAO Yi. Fixable Fluorescent Probes:Reliable Tools for Mitochondrial Sensing and Imaging[J]. Chinese Journal of Applied Chemistry, ;2017, 34(12): 1370-1378. doi: 10.11944/j.issn.1000-0518.2017.12.170303 shu

Fixable Fluorescent Probes:Reliable Tools for Mitochondrial Sensing and Imaging

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, Liaoning 116024, China

Corresponding author: XIAO Yi, xiaoyi@dlut.edu.cn
Received Date: 29 August 2017
Revised Date: 25 September 2017
Accepted Date: 30 September 2017

Fund Project: the National Natural Science Foundation of China 21576040the National Natural Science Foundation of China 21421005the National Natural Science Foundation of China 21174022the National Natural Science Foundation of China 21376038the National Basic Research Program of China 2013CB733702Supported by the National Natural Science Foundation of China(No.21174022, No.21376038, No.21421005, No.21576040), the National Basic Research Program of China(No.2013CB733702)

Figures(9)

Mitochondria, a cell organelle with a double membrane structure, plays significant roles in cell metabolism, including energy circulation and ions balance. Some small positively charged molecule fluorescent dyes/probes can label mitochondria due to the traction of the mitochondrial inner membrane negative potential, providing an important visual imaging tool for investigation of mitochondrial morphology and function. However, most mitochondrial dyes/probes are unsatisfactory as targeting marker of mitochondria, because the mitochondrial potential is constantly changing, when the potential reduced, the affinity of the dye accordingly decreases. Especially, the mitochondrial membrane potential will be significantly reduced when cell metabolism is blocked in pathological conditions(such as apoptosis), thus cationic dyes will spread away from mitochondria and result in non-specific fluorescence. Recently, the Kim team and our team proposed a new concept of fixed mitochondrial probe, with the active group of fluorescent molecular probe can be fixed in the mitochondria through the covalent bond, and developed a series of fluorescent probes that stably targeted mitochondria for the quantitative detection of microenvironment pH, viscosity, and membrane potential. We believe that the development of immobilized mitochondrial fluorescent molecular probes is an inevitable trend in tracking and detecting mitochondria with highly dynamic characteristics. Therefore, this paper reviews and prospects the fixable fluorescent dyes of mitochondria.
- mitochondria,
- fluorescence dyes,
- fluorescence mark,
- fixable
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