Citation: SHANG Jizhen, LI Zhi, XI Dongmei, ZHANG Shusheng. Progress in Biological Nanopore-based Analysis Technology[J]. Chinese Journal of Applied Chemistry, ;2017, 34(8): 855-867. doi: 10.11944/j.issn.1000-0518.2017.08.170103 shu

Progress in Biological Nanopore-based Analysis Technology

SHANG Jizhen ^a,b ,
LI Zhi ^b ,
XI Dongmei ^b,, ,
ZHANG Shusheng ^b,,

a.
The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165, China
b.
Shandong Provincial Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, Shangdong 276005, China

Corresponding author: XI Dongmei, dongmxi@126.com ZHANG Shusheng, shushzhang@126.com
Received Date: 5 April 2017
Revised Date: 17 May 2017
Accepted Date: 22 May 2017

Fund Project: Supported by the National Natural Science Foundation of China(No.21405073, No.21535002)the National Natural Science Foundation of China No.21405073the National Natural Science Foundation of China No.21535002

Figures(15)

Biological nanopore sensing has been developed as an attractive single-molecule tool with advantages of being rapid, low-cost, and label-free. Nanopores are not only being widely developed for DNA sequencing, but they also have been utilized to analyze a wide range of analytes at the single-molecule level. This review summarizes and prospects the principle of the nanopore analysis and nanpore category, with an emphasis on the progress in nanopore-based DNA sequencing and single-molecule analysis.
- biological nanopore,
- DNA sequencing,
- single-molecule analysis
1. [1]
  Kasianowicz J J, Brandin E, Branton D. Characterization of Individual Polynucleotide Molecules Using a Membrane Channel[J]. Proc Natl Acad Sci USA, 1996,93(24):13770-13773. doi: 10.1073/pnas.93.24.13770
2. [2]
  Branton D, Deamer D W, Marziali A. The Potential and Challenges of Nanopore Sequencing[J]. Nat Biotechnol, 2008,26(10):1146-1153. doi: 10.1038/nbt.1495
3. [3]
  Ying Y L, Zhang J, Gao R. Nanopore-based Sequencing and Detection of Nucleic Acids[J]. Angew Chem Int Ed, 2013,52(50):13154-13161. doi: 10.1002/anie.201303529
4. [4]
  Ying Y L, Cao C, Long Y T. Single Molecule Analysis by Biological Nanopore Sensors[J]. Analyst, 2014,139(16):3826-3835. doi: 10.1039/C4AN00706A
5. [5]
  Stefureac R, Long Y T, Kraatz H B. Transport of Alpha-helical Peptides Through Alpha-hemolysin and Aerolysin Pores[J]. Biochemistry, 2006,45(30):9172-9179. doi: 10.1021/bi0604835
6. [6]
  Butler T Z, Pavlenok M, Derrington I M. Single-molecule DNA Detection with an Engineered MspA Protein Nanopore[J]. Proc Natl Acad Sci USA, 2008,105(52):20647-20652. doi: 10.1073/pnas.0807514106
7. [7]
  Wendell D, Jing P, Geng J. Translocation of Double-stranded DNA Through Membrane-adapted phi29 Motor Protein Nanopores[J]. Nat Nanotechnol, 2009,4(11):765-772. doi: 10.1038/nnano.2009.259
8. [8]
  Wang H Y, Ying Y L, Li Y. Peering into Biological Nanopore:A Practical Technology to Single-molecule Analysis[J]. Chem Asian J, 2010,5(9):1952-1961. doi: 10.1002/asia.201000279
9. [9]
  Iacovache I, Paumard P, Scheib H. A Rivet Model for Channel Formation by Aerolysin-like Pore-forming Toxins[J]. EMBO J, 2006,25(3):457-466. doi: 10.1038/sj.emboj.7600959
10. [10]
  Song L, Hobaugh M R, Shustak C. Structure of Staphylococcal Alpha-hemolysin, a Heptameric Transmembrane Pore[J]. Science, 1996,274(5294):1859-1866. doi: 10.1126/science.274.5294.1859
11. [11]
  Akeson M, Branton D, Kasianowicz J J. Microsecond Time-scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA Molecules[J]. Biophys J, 1999,77(6):3227-3233. doi: 10.1016/S0006-3495(99)77153-5
12. [12]
  Pastoriza-Gallego M, Rabah L, Gibrat G. Dynamics of Unfolded Protein Transport Through an Aerolysin Pore[J]. J Am Chem Soc, 2011,133(9):2923-2931. doi: 10.1021/ja1073245
13. [13]
  Gouaux J E, Braha O, Hobaugh M R. Subunit Stoichiometry of Staphylococcal Alpha-hemolysin in Crystals and on Membranes:A Heptameric Transmembrane Pore[J]. Proc Natl Acad Sci USA, 1994,91(26):12828-12831. doi: 10.1073/pnas.91.26.12828
14. [14]
  Braha O, Walker B, Cheley S. Designed Protein Pores as Components for Biosensors[J]. Chem Biol, 1997,4(7):497-505. doi: 10.1016/S1074-5521(97)90321-5
15. [15]
  Parker M W, Buckley J T, Postma J P. Structure of the Aeromonas Toxin Proaerolysin in Its Water-soluble and Membrane-channel States[J]. Nature, 1994,367(6460):292-295. doi: 10.1038/367292a0
16. [16]
  Degiacomi M T, Iacovache I, Pernot L. Molecular Assembly of the Aerolysin Pore Reveals a Swirling Membrane-insertion Mechanism[J]. Nat Chem Biol, 2013,9(10):623-629. doi: 10.1038/nchembio.1312
17. [17]
  Merstorf C, Cressiot B, Pastoriza-Gallego M. Wild Type, Mutant Protein Unfolding and Phase Transition Detected by Single-nanopore Recording[J]. ACS Chem Biol, 2012,7(4):652-658. doi: 10.1021/cb2004737
18. [18]
  Cressiot B, Braselmann E, Oukhaled A. Dynamics and Energy Contributions for Transport of Unfolded Pertactin Through a Protein Nanopore[J]. ACS Nano, 2015,9(9):9050-9061. doi: 10.1021/acsnano.5b03053
19. [19]
  Fennouri A, Daniel R, Pastoriza-Gallego M. Kinetics of Enzymatic Degradation of High Molecular Weight Polysaccharides Through a Nanopore:Experiments and Data-modeling[J]. Anal Chem, 2013,85(18):8488-8492. doi: 10.1021/ac4020929
20. [20]
  Derrington I M, Butler T Z, Collins M D. Nanopore DNA Sequencing with MspA[J]. Proc Natl Acad Sci USA, 2010,107(37):16060-16065. doi: 10.1073/pnas.1001831107
21. [21]
  Heinz C, Karosi S, Niederweis M. High-level Expression of the Mycobacterial Porin MspA in Escherichia coli and Purification of the Recombinant Protein[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2003,790(1/2):337-348.
22. [22]
  Guasch A, Pous J, Ibarra B. Detailed Architecture of a DNA Translocating Machine:The High-resolution Structure of the Bacteriophage phi29 Connector Particle[J]. J Mol Biol, 2002,315(4):663-676. doi: 10.1006/jmbi.2001.5278
23. [23]
  Jing P, Haque F, Vonderheide A P. Robust Properties of Membrane-embedded Connector Channel of Bacterial Virus phi29 DNA Packaging Motor[J]. Mol Biosyst, 2010,6(10):1844-1852. doi: 10.1039/c003010d
24. [24]
  Kawano R, Schibel A E, Cauley C. Controlling the Translocation of Single-stranded DNA Through Alpha-hemolysin Ion Channels Using Viscosity[J]. Langmuir, 2009,25(2):1233-1237. doi: 10.1021/la803556p
25. [25]
  Meller A, Nivon L, Brandin E. Rapid Nanopore Discrimination Between Single Polynucleotide Molecules[J]. Proc Natl Acad Sci USA, 2000,97(3):1079-1084. doi: 10.1073/pnas.97.3.1079
26. [26]
  Rincon-Restrepo M, Mikhailova E, Bayley H. Controlled Translocation of Individual DNA Molecules Through Protein Nanopores with Engineered Molecular Brakes[J]. Nano Lett, 2011,11(2):746-750. doi: 10.1021/nl1038874
27. [27]
  Cherf G M, Lieberman K R, Rashid H. Automated forward and Reverse Ratcheting of DNA in a Nanopore at 5-A Precision[J]. Nat Biotechnol, 2012,30(4):344-348. doi: 10.1038/nbt.2147
28. [28]
  Lieberman K R, Cherf G M, Doody M J. Processive Replication of Single DNA Molecules in a Nanopore Catalyzed by phi29 DNA Polymerase[J]. J Am Chem Soc, 2010,132(50):17961-17972. doi: 10.1021/ja1087612
29. [29]
  Stoddart D, Maglia G, Mikhailova E. Multiple Base-recognition Sites in a Biological Nanopore:Two Heads are Better than One[J]. Angew Chem Int Ed, 2010,49(3):556-559. doi: 10.1002/anie.200905483
30. [30]
  Gu L Q, Cheley S, Bayley H. Capture of a Single Molecule in a Nanocavity[J]. Science, 2001,291(5504):636-640. doi: 10.1126/science.291.5504.636
31. [31]
  Gu L Q, Braha O, Conlan S. Stochastic Sensing of Organic Analytes by a Pore-forming Protein Containing a Molecular Adapter[J]. Nature, 1999,398(6729):686-690. doi: 10.1038/19491
32. [32]
  Clarke J, Wu H C, Jayasinghe L. Continuous Base Identification for Single-molecule Nanopore DNA Sequencing[J]. Nat Nanotechnol, 2009,4(4):265-270. doi: 10.1038/nnano.2009.12
33. [33]
  Kumar S, Tao C, Chien M. PEG-labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis[J]. Sci Rep, 2012,2684. doi: 10.1038/srep00684
34. [34]
  Manrao E A, Derrington I M, Laszlo A H. Reading DNA at Single-nucleotide Resolution with a Mutant MspA Nanopore and phi29 DNA Polymerase[J]. Nat Biotechnol, 2012,30(4):349-353. doi: 10.1038/nbt.2171
35. [35]
  Pennisi E. Genome Sequencing[J]. Science, 2012,336(6081):534-537. doi: 10.1126/science.336.6081.534
36. [36]
  Stranges P B, Palla M, Kalachikov S. Design and Characterization of a Nanopore-coupled Polymerase for Single-molecule DNA Sequencing by Synthesis on an Electrode Array[J]. Proc Natl Acad Sci USA, 2016,113(44):E6749-E6756. doi: 10.1073/pnas.1608271113
37. [37]
  Henrickson S E, Misakian M, Robertson B. Driven DNA Transport into an Asymmetric Nanometer-scale Pore[J]. Phys Rev Lett, 2000,85(14):3057-3060. doi: 10.1103/PhysRevLett.85.3057
38. [38]
  Sauer-Budge A F, Nyamwanda J A, Lubensky D K. Unzipping Kinetics of Double-stranded DNA in a Nanopore[J]. Phys Rev Lett, 2003,90(23)238101. doi: 10.1103/PhysRevLett.90.238101
39. [39]
  Bates M, Burns M, Meller A. Dynamics of DNA Molecules in a Membrane Channel Probed by Active Control Techniques[J]. Biophys J, 2003,84(4):2366-2372. doi: 10.1016/S0006-3495(03)75042-5
40. [40]
  Wang Y, Zheng D, Tan Q. Nanopore-based Detection of Circulating MicroRNAs in Lung Cancer Patients[J]. Nat Nanotechnol, 2011,6(10):668-674. doi: 10.1038/nnano.2011.147
41. [41]
  Xi D, Shang J, Fan E. Nanopore-Based Selective Discrimination of MicroRNAs with Single-Nucleotide Difference Using Locked Nucleic Acid-Modified Probes[J]. Anal Chem, 2016,88(21):10540-10546. doi: 10.1021/acs.analchem.6b02620
42. [42]
  Tian K, Decker K, Aksimentiev A. Interference-Free Detection of Genetic Biomarkers Using Synthetic Dipole-Facilitated Nanopore Dielectrophoresis[J]. ACS Nano, 2017,11(2):1204-1213. doi: 10.1021/acsnano.6b07570
43. [43]
  Cao C, Ying Y L, Hu Z L. Discrimination of Oligonucleotides of Different Lengths with a Wild-type Aerolysin Nanopore[J]. Nat Nanotechnol, 2016,11(8):713-718. doi: 10.1038/nnano.2016.66
44. [44]
  Movileanu L, Schmittschmitt J P, Scholtz J M. Interactions of Peptides with a Protein Pore[J]. Biophys J, 2005,89(2):1030-1045. doi: 10.1529/biophysj.104.057406
45. [45]
  Mohammad M M, Prakash S, Matouschek A. Controlling a Single Protein in a Nanopore Through Electrostatic Traps[J]. J Am Chem Soc, 2008,130(12):4081-4088. doi: 10.1021/ja710787a
46. [46]
  Wolfe A J, Mohammad M M, Cheley S. Catalyzing the Translocation of Polypeptides Through Attractive Interactions[J]. J Am Chem Soc, 2007,129(45):14034-14041. doi: 10.1021/ja0749340
47. [47]
  Wang H Y, Ying Y L, Li Y. Nanopore Analysis of Beta-amyloid Peptide Aggregation Transition Induced by Small Molecules[J]. Anal Chem, 2011,83(5):1746-1752. doi: 10.1021/ac1029874
48. [48]
  Wang H Y, Gu Z, Cao C. Analysis of a Single Alpha-synuclein Fibrillation by the Interaction with a Protein Nanopore[J]. Anal Chem, 2013,85(17):8254-8261. doi: 10.1021/ac401496x
49. [49]
  Rodriguez-Larrea D, Bayley H. Multistep Protein Unfolding During Nanopore Translocation[J]. Nat Nanotechnol, 2013,8(4):288-295. doi: 10.1038/nnano.2013.22
50. [50]
  Rosen C B, Rodriguez-Larrea D, Bayley H. Single-molecule Site-specific Detection of Protein Phosphorylation with a Nanopore[J]. Nat Biotechnol, 2014,32(2):179-181. doi: 10.1038/nbt.2799
51. [51]
  Soskine M, Biesemans A, De Maeyer M. Tuning the Size and Properties of ClyA Nanopores Assisted by Directed Evolution[J]. J Am Chem Soc, 2013,135(36):13456-13463. doi: 10.1021/ja4053398
52. [52]
  Fahie M, Chisholm C, Chen M. Resolved Single-molecule Detection of Individual Species Within a Mixture of Anti-biotin Antibodies Using an Engineered Monomeric Nanopore[J]. ACS Nano, 2015,9(2):1089-1098. doi: 10.1021/nn506606e
53. [53]
  Zhang X, Xu X, Yang Z. Mimicking Ribosomal Unfolding of RNA Pseudoknot in a Protein Channel[J]. J Am Chem Soc, 2015,137(50):15742-15752. doi: 10.1021/jacs.5b07910
54. [54]
  Zhang L, Zhang K, Liu G. Label-free Nanopore Proximity Bioassay for Platelet-derived Growth Factor Detection[J]. Anal Chem, 2015,87(11):5677-5682. doi: 10.1021/acs.analchem.5b00791
55. [55]
  Li T, Liu L, Li Y. A Universal Strategy for Aptamer-based Nanopore Sensing Through Host-guest Interactions Inside Alpha-hemolysin[J]. Angew Chem Int Ed, 2015,54(26):7568-7571. doi: 10.1002/anie.201502047
56. [56]
  Braha O, Webb J, Gu L Q. Carriers Versus Adapters in Stochastic Sensing[J]. ChemPhysChem, 2005,6(5):889-892. doi: 10.1002/(ISSN)1439-7641
57. [57]
  Braha O, Gu L Q, Zhou L. Simultaneous Stochastic Sensing of Divalent Metal Ions[J]. Nat Biotechnol, 2000,18(9):1005-1007. doi: 10.1038/79275
58. [58]
  Wen S, Zeng T, Liu L. Highly Sensitive and Selective DNA-based Detection of Mercury(Ⅱ) with Alpha-hemolysin Nanopore[J]. J Am Chem Soc, 2011,133(45):18312-18317. doi: 10.1021/ja206983z
59. [59]
  Yang C, Liu L, Zeng T. Highly Sensitive Simultaneous Detection of Lead(Ⅱ) and Barium(Ⅱ) with G-quadruplex DNA in Alpha-hemolysin Nanopore[J]. Anal Chem, 2013,85(15):7302-7307. doi: 10.1021/ac401198d
60. [60]
  Huang S, Romero-Ruiz M, Castell O K. High-throughput Optical Sensing of Nucleic Acids in a Nanopore Array[J]. Nat Nanotechnol, 2015,10(11):986-991. doi: 10.1038/nnano.2015.189
61. [61]
  Gu Z, Ying Y L, Cao C. Accurate Data Process for Nanopore Analysis[J]. Anal Chem, 2015,87(2):907-913. doi: 10.1021/ac5028758
62. [62]
  Zhang J, Liu X, Ying Y L. High-bandwidth Nanopore Data Analysis by Using a Modified Hidden Markov Model[J]. Nanoscale, 2017,9(10):3458-3465. doi: 10.1039/C6NR09135K
63. [63]
  Gao R, Ying Y L, Yan B Y. An Integrated Current Measurement System for Nanopore Analysis[J]. Chinese Sci Bull, 2014,59(35):4968-4973. doi: 10.1007/s11434-014-0656-0
1. [1]
  . . Chinese Journal of Inorganic Chemistry, 2024, 40(12): 0-0.
2. [2]
  Jingwen Wang , Minghao Wu , Xing Zuo , Yaofeng Yuan , Yahao Wang , Xiaoshun Zhou , Jianfeng Yan . Advances in the Application of Electrochemical Regulation in Investigating the Electron Transport Properties of Single-Molecule Junctions. University Chemistry, 2025, 40(3): 291-301. doi: 10.12461/PKU.DXHX202406023
3. [3]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
4. [4]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
5. [5]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249
6. [6]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
7. [7]
  Shiyang He , Dandan Chu , Zhixin Pang , Yuhang Du , Jiayi Wang , Yuhong Chen , Yumeng Su , Jianhua Qin , Xiangrong Pan , Zhan Zhou , Jingguo Li , Lufang Ma , Chaoliang Tan . 铂单原子功能化的二维Al-TCPP金属-有机框架纳米片用于增强光动力抗菌治疗. Acta Physico-Chimica Sinica, 2025, 41(5): 100046-. doi: 10.1016/j.actphy.2025.100046
8. [8]
  Wenyan Dan , Weijie Li , Xiaogang Wang . The Technical Analysis of Visual Software ShelXle for Refinement of Small Molecular Crystal Structure. University Chemistry, 2024, 39(3): 63-69. doi: 10.3866/PKU.DXHX202302060
9. [9]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
10. [10]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
11. [11]
  Meiqing Yang , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-. doi: 10.3866/PKU.WHXB202310046
12. [12]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
13. [13]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
14. [14]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
15. [15]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
16. [16]
  Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
17. [17]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
18. [18]
  Zhongyu Wang , Lijun Wang , Huaixin Zhao . DNA-based nanosystems to generate reactive oxygen species for nanomedicine. Chinese Chemical Letters, 2024, 35(11): 109637-. doi: 10.1016/j.cclet.2024.109637
19. [19]
  Jiangshan Xu , Weifei Zhang , Zhengwen Cai , Yong Li , Long Bai , Shaojingya Gao , Qiang Sun , Yunfeng Lin . Tetrahedron DNA nanostructure/iron-based nanomaterials for combined tumor therapy. Chinese Chemical Letters, 2024, 35(11): 109620-. doi: 10.1016/j.cclet.2024.109620
20. [20]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094

Get Citation

PDF

XML

Metrics

PDF Downloads(29)
Abstract views(1762)
HTML views(550)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142